Involvement of Crustacean Hyperglycemic Hormone in the Control of Gill Ion Transport in the Crab Pachygrapsus marmoratus

Spanings-Pierrot, Céline; Soyez, Daniel; Herp, F. Van; Gompel, Marie; Skaret, Georg; Grousset, Evelyse; Charmantier, Guy

doi:10.1006/gcen.2000.7527

Cited by 128 publications

(78 citation statements)

References 46 publications

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“…It remains unclear whether the measured increases in activity result from de novo synthesis of Na + /K + -ATPase mRNA and/or protein or from post-translational processes such as subunit assembly, membrane trafficking and cell signaling 12 . This study does not clarify the mechanisms for Na + / K + -ATPase activity in P. monodon as well as the reports in other crustaceans 9,11,23 . In the method for determination of gene expression, RT-PCR, PCR band intensity reflects relative transcript abundance in the experimental RNA samples.…”

Section: Discussioncontrasting

confidence: 96%

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Wuthisuthimethavee¹,

Lunubol²,

Vanavichit³

2005

ScienceAsia

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Expressed sequence tag (EST) analysis is a powerful tool for gene discovery. A preliminary study of genes expressed in branchiae of Penaeus monodon comprised constructing a cDNA library, cDNA sequencing and a homology search of public databases. The constructed cDNA library contained insert sizes ranging from 0.5-1.6 kb. From 200 randomly selected EST clones, 176 (88%) were successfully sequenced and gave average read-length after vector clipping of 325 bp. A total of 26 contigs were formed after clustering the 176 ESTs. The contigs contained a total of 72 EST sequences and 104 sequences remained as singleton ESTs. Bioinformatics analysis revealed that 42 from 104 transcripts matched known genes. These were categorized into 6 classes of genes involved in gene/protein expression (50%), metabolism (23.8%), cell/organism defense (9.6%), cell structure/motility (7.1%), and cell signaling and communication (2.4%). Genes lacking enough information to be classified constituted the remaining 7.1%. This study also found a transcript with high homology to an Na + /K + -ATPase gene reported to be involved in osmoregulation of crustaceans, and especially to the α-subunit Na + /K + -ATPase in H. americanus. Expression of this gene in branchiae of low salinity-stressed P. monodon was examined using RT-PCR analysis. The results showed that the mRNA abundance in stressed shrimps decreased over 7 days in contrast to a previously-described increase in the enzyme activity.

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Section: Discussioncontrasting

confidence: 96%

“…Many studies have shown that gills are the primary organs for salt transport in crabs 8,9,10 and lobsters 11 . They utilize apical membrane branchial Na /HCO 3 -exchange powered by a basal membrane Na + / K + -ATPase.…”

Section: Introductionmentioning

confidence: 99%

Untitled

Wuthisuthimethavee¹,

Lunubol²,

Vanavichit³

2005

ScienceAsia

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“…Gills were previously shown to be the main tissue involved in regulation of osmotic pressure and sodium ion levels in the circulation (Spanings-Pierrot et al, 2000). Generally, the effect of CHH on the gill gene expression profile encompassed, upon application of the hormones, less than 0.3% of the transcript population.…”

Section: Research Articlementioning

confidence: 99%

Different transcription regulation routes are exerted by L- and D-amino acid enantiomers of peptide hormones

Tom

Manfrin

Mosco

et al. 2014

Journal of Experimental Biology

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Conversion of one or more amino acids in eukaryotic peptides to the D-enantiomer configuration is catalyzed by specific L/D-peptide isomerases and it is a poorly investigated post-translational modification. No common modified amino acid or specific modified position has been recognized, and mechanisms underlying changes in the peptide function provided by this conversion are not widely studied. The 72 amino acid crustacean hyperglycemic hormone (CHH) in Astacidea crustaceans exhibits a co-existence of two peptide enantiomers with either D-or L-phenylalanine as their third residue. It is a pleiotropic hormone regulating several physiological processes in different target tissues and along different time scales. CHH enantiomers differently affect time courses and intensities of examined processes. The short-term effects of the two isomers on gene expression were examined in the hepatopancreas, gills, hemocytes and muscles of the astacid Pontastacus leptodactylus. Gene expression in muscles and hemocytes was not affected by either of the isomers. Two modes of action for CHH were elucidated in the hepatopancreas and the gills: specific gene induction in both organs by D-CHH, and targeted attenuation caused by both enantiomers in the gills. Consequently, a two-receptor system is proposed for conveying the effect of the two CHH isomers.

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“…Thus, CHH could potentially increase ion uptake and thus osmoregulatory capacity at the gills by increasing the availability of metabolisable energy to the ion-exchange pumps at the gills through increased glycogenolysis (Spanings-Pierrot et al, 2000).…”

Section: (N=7) (Studentʼs T-tests and Mann-whitney Tests) (C-f) Glmentioning

confidence: 99%

“…A subject of topical interest is the involvement of CHH in ionoregulatory processes (Charmantier-Daures et al, 1994;Charmantier et al, 1999;Chung et al, 1999;Townsend et al, 2001;Serrano et al, 2003;Chung and Webster, 2006). Whilst it is known that sinus gland extracts cause rapid and reversible changes in transepithelial potentials, and Na + transport in isolated perfused gills of Pachygrapsus marmoratus (Spanings-Pierrot et al, 2000), the precise mechanisms regulated by CHH in crustacean gill tissues remain poorly investigated, despite our extensive knowledge of branchial membrane exchangers and pumps (Lucu, 1990;Onken et al, 1991;Onken and Putzenlechner, 1995;Lucu and Towle, 2003;Weihrauch et al, 2004;Tsai and Lin, 2007;Masui et al, 2008). Additionally, because the insect equivalent of CHH-ion transport peptide (ITP) has long been known to stimulate water uptake in the locust hindgut by opening Cl -channels (Phillips and Audsley, 1995;Phillips et al, 1998), it is surprising that the ionoregulatory processes controlled by CHH have not attracted further interest.…”

Section: Introductionmentioning

confidence: 99%

Roles of crustacean hyperglycaemic hormone in ionic and metabolic homeostasis in the Christmas Island Blue crabDiscoplax celeste

Turner

Webster

Morris

2012

Journal of Experimental Biology

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SUMMARYThere is a growing body of evidence implicating the involvement of crustacean hyperglycaemic hormone (CHH) in ionic homeostasis in decapod crustaceans. However, little is known regarding hormonally influenced osmoregulatory processes in terrestrial decapods. As many terrestrial decapods experience opposing seasonal demands upon ionoregulatory physiologies, we reasoned that these would make interesting models in which to study the effect of CHH upon these phenomena. In particular, those (tropical) species that also undergo seasonal migrations might be especially informative, as we know relatively little regarding the nature of CHHs in terrestrial decapods, and hormonally mediated responses to seasonal changes in metabolic demands might also be superimposed or otherwise integrated with those associated with ionic homeostasis. Using Discoplax celeste as a model crab that experiences seasonal extremes in water availability, and exhibits diurnal and migratory activity patterns, we identified two CHHs in the sinus gland. We biochemically characterised (cDNA cloning) one CHH and functionally characterised (in terms of dose-dependent hyperglycaemic responses and glucose-dependent negative feedback loops) both CHHs. Whole-animal in situ branchial chamber 22 NaCl perfusion experiments showed that injection of both CHHs increased gill Na + uptake in a seasonally dependent manner, and 51Cr-EDTA clearance experiments demonstrated that CHH increased urine production by the antennal gland. Seasonal and salinity-dependent differences in haemolymph CHH titre further implicated CHH in osmoregulatory processes. Intriguingly, CHH appeared to have no effect on gill Na + /K + -ATPase or V-ATPase activity, suggesting unknown mechanisms of this hormoneʼs action on Na + transport across gill epithelia.Supplementary material available online at

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Involvement of Crustacean Hyperglycemic Hormone in the Control of Gill Ion Transport in the Crab Pachygrapsus marmoratus

Cited by 128 publications

References 46 publications

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Different transcription regulation routes are exerted by L- and D-amino acid enantiomers of peptide hormones

Roles of crustacean hyperglycaemic hormone in ionic and metabolic homeostasis in the Christmas Island Blue crabDiscoplax celeste

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