Involvement of caspase-3 activation in squamocin-induced apoptosis in leukemia cell line HL-60

Zhu, Xiao Feng; Liu, Zong Chao; Xie, Bingteng; Li, Zhi Ming; Feng, Gong Kan; Xie, Hai Hui; Wu, Shu Jun; Ren, Yang; Wei, Xiao; Zeng, Yi Xin

doi:10.1016/s0024-3205(01)01501-6

Cited by 33 publications

(21 citation statements)

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“…Apoptosis, programmed cell death, is a fundamental mode of cell death. Phosphatidyl serine translocation to the cell surface is an indicator of early apoptotic cells (Zhu et al, 2002). With Annexin V staining assay, both etoposide and excisanin A significantly increased the Annexin V-positive cells in SW620 cell line (Figure 1 (i) and (ii)).…”

Section: Discussionmentioning

confidence: 95%

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Acetylcholinesterase expression mediated by c-Jun-NH2-terminal kinase pathway during anticancer drug-induced apoptosis

Deng

Guan

et al. 2006

Oncogene

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Section: Discussionmentioning

confidence: 95%

“…Excisanin A from plant Largesepal Rabdosia was initially dissolved in 100% dimethylsulfoxide (DMSO) and stored at À201C (Gao et al, 2000;Zhu et al, 2002). RPMI-1640 medium, DMSO and sodium dodeeyl sulfate (SDS) were purchased from Sigma.…”

Section: Drugs and Reagentsmentioning

confidence: 99%

Acetylcholinesterase expression mediated by c-Jun-NH2-terminal kinase pathway during anticancer drug-induced apoptosis

Deng

Guan

et al. 2006

Oncogene

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“…A Chinese herbal medicine Takrisodokyeum (TRSDY), known to exert anti-tumoral activity in Korea, induces hydrogen peroxide generation, which, in turn, causes activation of caspase-3, degradation of PARP and D4-GDI, and eventually leads to apoptotic cell death (40). Apoptosis of HL-60 cells induced by squamocin requires caspase-3 activation and is related to stress-activated protein kinase (SAPK) activation (41). Wogonin and fisetin had effective cytotoxic effects through the induction of apoptosis in hepatocellular carcinoma cells SK-HEP-1; activation of caspase 3 cascade, induction of p53 protein, and alternative expression of p21 (Waf/ Cip-1) protein were involved (42).…”

Section: Discussionmentioning

confidence: 99%

Induction of Apoptosis by Korean Medicine Gagam-whanglyun-haedoktang Through Activation of Caspase-3 in Human Leukemia Cell Line, HL-60 Cells

et al. 2005

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Abstract. Gagam-whanglyun-haedoktang (GWH) is a newly designed herbal drug formula based on the traditional oriental pharmacological knowledge for the purpose of treating tumorous diseases. Apoptosis is an evolutionarily conserved suicide program residing in cells. In the present study, apoptosis inducing activities of the decocted water extract of GWH were studied. Results of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that GWH had a strong cytotoxic effect on HL-60 cells. The number of live cells was less than 20% after exposure to 1 mg / ml GWH for 48 h. GWH increased cytotoxicity of HL-60 cells in a doseand time-dependent manner. The percentage of apoptotic cells by flow cytometric analysis of the DNA-stained cells increased to 28%, 31%, and 37% at 24 h and to 37%, 44%, and 81% at 48 h after treatment with 0.01, 0.1, and 1 mg / ml GWH, respectively. DNA fragmentation also occurred in apoptosis and was characterized by a ladder pattern on agarose gel. In addition, GWH increased the secretion of tumor necrosis factor-a . GWH-induced apoptosis was accompanied by activation of caspase-3. These results suggest that GWH induces activation of caspase-3 and eventually leads to apoptosis.

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“…102 Esses são alguns exemplos de trabalhos que associam as ACGs à apoptose, fazendo com o que os membros desta classe de compostos naturais sejam considerados objetos de futuras gerações de fármacos anticâncer. …”

Section: 99100unclassified

Cytotoxic Potencial Acetogenins ofAnnonaGenus

Paes¹,

Vega²,

Cortés³

et al. 2016

Revista Virtual De Química

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Cytotoxic Potencial Acetogenins of Annona GenusAbstract: Over the centuries, nature has contributed to the basic needs of human beings. In particular, plants have cooperated as a source of drugs for the treatment of a broad spectrum of diseases because of the structural diversity of secondary metabolites. With strict attention to antineoplastic therapy, the search for anticancer drugs has increased in order to meet more specific and selective treatments. The acetogenins (ACGs), a class of secondary metabolites found exclusively in the family Annonaceae, are substances of great importance due to their well-known cytotoxic potency in human tumor cell lines in vitro, which can be exploited in designing anticancer agents. Among the 150 genera of Annonaceae family, genus Annona is in which is recorded the highest number of these substances. In order to identify the therapeutic potential and future directions for research, the purpose of this review is to provide data pertaining to the studies of cytotoxicity in vitro in cell lines of human cancer of isolated ACGs of Annona species in the period between 1989 and 2012 as well as basic information regarding the phytochemical studies of the same.

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Involvement of caspase-3 activation in squamocin-induced apoptosis in leukemia cell line HL-60

Cited by 33 publications

References 0 publications

Acetylcholinesterase expression mediated by c-Jun-NH2-terminal kinase pathway during anticancer drug-induced apoptosis

Acetylcholinesterase expression mediated by c-Jun-NH2-terminal kinase pathway during anticancer drug-induced apoptosis

Induction of Apoptosis by Korean Medicine Gagam-whanglyun-haedoktang Through Activation of Caspase-3 in Human Leukemia Cell Line, HL-60 Cells

Cytotoxic Potencial Acetogenins ofAnnonaGenus

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