Involvement of ABA- and H 2 O 2 -dependent cytosolic glucose-6-phosphate dehydrogenase in maintaining redox homeostasis in soybean roots under drought stress

Wang, Huahua; Yang, Liting; Li, Yan; Hou, Junjie; Huang, Junjun; Liang, Weihong

doi:10.1016/j.plaphy.2016.05.040

Cited by 52 publications

(48 citation statements)

References 59 publications

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“…In the present work, an apparent elevation in the levels of antioxidants (AsA and GSH) as well as the ratios of AsA/DHA and GSH/GSSG were seen in GmG6PDH2-OHR plants related to the CHR plants ( Figures 9A,B). These data validate that GmG6PDH2 plays a pivotal role in modulating the changes of metabolite contents and the redox state of AsA and GSH pool under salinity condition, which was consistent with previous results (Krüger et al, 2011;Wang et al, 2016). It is known that the AsA-GSH cycle has a main function of eliminating H 2 O 2 , a potentially harmful ROS (Fotopoulos et al, 2010;Li et al, 2010).…”

Section: Discussionsupporting

confidence: 92%

“…Besides, overexpression of a cytosolic PsG6PDH gene from Populus suaveolens confers an increased cold tolerance in transgenic tobacco by elevating the activity of antioxidative enzymes, such as peroxidase and superoxide dismutase, and decreasing the level of membrane lipid peroxidation (Lin et al, 2005(Lin et al, , 2013. Also, the enhanced cytosolic G6PDH activities would contribute to the improvement of drought tolerance in soybean roots, with the involvement of ABA-dependent signaling pathway (Liu et al, 2013;Wang et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Analysis of the Glucose-6-Phosphate Dehydrogenase Family in Soybean and Functional Identification of GmG6PDH2 Involvement in Salt Stress

et al. 2020

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Glucose-6-phosphate dehydrogenase (G6PDH) is known as a critical enzyme responsible for nicotinamide adenine dinucleotide phosphate (NADPH) generation in the pentose phosphate pathway (PPP), and has an essential function in modulating redox homeostasis and stress responsiveness. In the present work, we characterized the nine members of the G6PDH gene family in soybean. Phylogenic analysis and transit peptide prediction showed that these soybean G6PDHs are divided into plastidic (P) and cytosolic (Cy) isoforms. The subcellular locations of five GmG6PDHs were further verified by confocal microscopy in Arabidopsis mesophyll protoplasts. The respective GmG6PDH genes had distinct expression patterns in various soybean tissues and at different times during seed development. Among them, the Cy-G6PDHs were strongly expressed in roots, developing seeds and nodules, while the transcripts of P-G6PDHs were mainly detected in green tissues. In addition, the activities and transcripts of GmG6PDHs were dramatically stimulated by different stress treatments, including salt, osmotic and alkali. Notably, the expression levels of a cytosolic isoform (GmG6PDH2) were extraordinarily high under salt stress and correlated well with the G6PDH enzyme activities, possibly implying a crucial factor for soybean responses to salinity. Enzymatic assay of recombinant GmG6PDH2 proteins expressed in Escherichia coli showed that the enzyme encoded by GmG6PDH2 had functional NADP +-dependent G6PDH activity. Further analysis indicated overexpression of GmG6PDH2 gene could significantly enhance the resistance of transgenic soybean to salt stress by coordinating with the redox states of ascorbic acid and glutathione pool to suppress reactive oxygen species generation. Together, these results indicate that GmG6PDH2 might be the major isoform for NADPH production in PPP, which is involved in the modulation of cellular AsA-GSH cycle to prevent the oxidative damage induced by high salinity.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis of the Glucose-6-Phosphate Dehydrogenase Family in Soybean and Functional Identification of GmG6PDH2 Involvement in Salt Stress

et al. 2020

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“…In soybean plants, we observed that HS neutralized oxidative stress by decreasing H 2 O 2 levels and modulating the ABA signaling pathway, allowing the maintenance of gs. This is because ABA induces H 2 O 2 production for stimulating NADPH oxidase activity under drought stress (Wang et al ). Similarly, increases in gas exchange have been reported with the addition of HS in plants of spinach (Chen et al ) and the application of ZS in wheat plants under drought conditions (Karim et al ).…”

Section: Discussionmentioning

confidence: 99%

Biochemical and physiological impacts of zinc sulphate, potassium phosphite and hydrogen sulphide in mitigating stress conditions in soybean

Batista

Müller

Merchant

et al. 2020

Physiologia Plantarum

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Abbreviations -A, net photosynthetic assimilation rate; APX, ascorbate peroxidase; CAT, catalase; Chl a, chlorophyll a; Chl b, chlorophyll b; Chl a/Chl b, ratio between Chl a and Chl b; E, transpiration rate; ETR, electron transport rate; ETR/A, electron transport to oxygen molecules; F0, initial fluorescence; Fv/fm, potential quantum yield of photosystem II; g s , stomatal conductance; H 2 O 2 , hydrogen peroxide; HS, hydrogen sulphide; LDM, leaf dry matter; MDA, malondialdehyde; MWHC, maximum water holding capacity; NPQ, non-photochemical quenching coefficient; POX, peroxidase activity; PP, potassium phosphite; RDW, root dry weight; REL, rate of electrolyte leakage; RWC, relative water content; SDM, shoot dry matter; SOD, superoxide dismutase; TDM, total dry matter; WD, water deficit; WUEg, intrinsic water use efficiency; WW, well-watered; ZS, zinc sulphate; Ψ s , osmotic potential; Ψ w , water potential; Φ PSII , effective quantum yield of photosystem II.

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“…Respond to oxidative stress, plants evolved complex acclimation and defence strategies to minimize the deleterious effects due to excess ROS [ 41 ]. It has been proposed that ASA, GSH, and the enzymes including APX, GR, DHAR, MDHAR, and GPX involved in ASA-GSH metabolism enable to act as ROS scavengers [ 76 , 77 ], and the involvement of the ASA-GSH cycle in the protection of oxidative damage caused by drought to plants has also been observed in Fargesia rufa [ 78 ], soybean [ 79 ], Cerasus humilis [ 33 ]. Unfortunately, none of these studies tried to elucidate ROS defence and redox regulation mechanisms in wheat spike bracts under drought conditions in detail.…”

Section: Discussionmentioning

confidence: 99%

Photosynthetic and ascorbate-glutathione metabolism in the flag leaves as compared to spikes under drought stress of winter wheat (Triticum aestivum L.)

Lou

Chen

et al. 2018

PLoS ONE

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Ascorbate-glutathione (ASA-GSH) cycle is a major pathway of H2O2 scavenging and an effective mechanism of detoxification in plants. The differences in photosynthesis, chlorophyll content (Chl), relative water content (RWC), antioxidants and antioxidative enzyme activities involved in ASA-GSH metabolism were measured between the flag leaves and spike bracts (glumes and lemmas) during grain filling under drought stress. The expression of APX1, GRC1, DHAR, MDHAR, GPX1, and GS3 in ASA-GSH cycle was also measured. Compared with the flag leaves, the spike bracts exhibited stable net photosynthetic rate (PN) and chlorophyll content (Chl), a lower accumulation of reactive oxygen species (ROS), and more enhanced percentages of antioxidant enzyme activities and key enzymes gene transcription levels involved in ASA-GSH metabolism during the grain-filling stage under drought conditions. This could be the reasonable explanation for the more stable photosynthetic capacity in spikes, and the glumes and lemmas senesced later than the flag leaves at the late grain-filling stage. Also, the function of ASA-GSH cycle could not be ignored in alleviating oxidative damage by scavenging more excess ROS in spikes under drought stress.

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Involvement of ABA- and H 2 O 2 -dependent cytosolic glucose-6-phosphate dehydrogenase in maintaining redox homeostasis in soybean roots under drought stress

Cited by 52 publications

References 59 publications

Genome-Wide Analysis of the Glucose-6-Phosphate Dehydrogenase Family in Soybean and Functional Identification of GmG6PDH2 Involvement in Salt Stress

Genome-Wide Analysis of the Glucose-6-Phosphate Dehydrogenase Family in Soybean and Functional Identification of GmG6PDH2 Involvement in Salt Stress

Biochemical and physiological impacts of zinc sulphate, potassium phosphite and hydrogen sulphide in mitigating stress conditions in soybean

Photosynthetic and ascorbate-glutathione metabolism in the flag leaves as compared to spikes under drought stress of winter wheat (Triticum aestivum L.)

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