Investigations toward new lead compounds from medicinally important plants

Prasad, Ashok K.; Kumar, Vineet; Arya, Pragya; Kumar, Sarvesh; Dabur, Rajesh; Singh, Naresh; Chhillar, Anil Kumar; Sharma, Gainda L.; Ghosh, Balaram; Wengel, Jesper; Olsen, Carl Erik; Parmar, Virinder S.

doi:10.1351/pac200577010025

Cited by 30 publications

(12 citation statements)

References 2 publications

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“…Various piper species (piperaceae) have been used as spices and also as folk medicines for hepotensia, abdominal pain and anthelminthic 6–8. The main bioactive constituents of piper species are amides,9–23 and it is known that amides can be characterized well by MS 24.…”

mentioning

confidence: 99%

Rapid structural determination of amides in Piper longum by high‐performance liquid chromatography combined with ion trap mass spectrometry

Sun¹,

Pei²,

Pan³

2007

Rapid Comm Mass Spectrometry

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A reversed-phase liquid chromatography/tandem multistage mass spectrometry (MS/MS) method was developed for the characterization of amides from the extracts of Piper longum. The characteristic fragmentations of the amides found in P. longum showed diagnostic structural information. Extracted ion chromatography (EIC) and constant neutral losses were used to guide the search for the amides of interest. Amides of known structures that contain four subtypes of amides were rapidly determined, and novel amides were also identified for this plant. Forty-two amides were rapidly identified, of which 22 were found in this plant for the first time and 9 were new compounds. The method is convenient and sensitive, especially for minor components in the unpurified, complex mixture; the structures of unknown constituents could be determined, in the absence of authentic sample, by comparison of the fragmentation patterns with those of homologous compounds.

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confidence: 99%

Rapid structural determination of amides in Piper longum by high‐performance liquid chromatography combined with ion trap mass spectrometry

Sun¹,

Pei²,

Pan³

2007

Rapid Comm Mass Spectrometry

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“…The presence of b-myrcene was also recorded in the essential oil from Artemisia herba and lemon grass [25,26] and leaves and flower of Bidens pilosa [27]. A number of bioactive compounds including fungicidal compound, pipernonaline have been isolated from P. longum [11,13,28,29]. However, most of the compounds were isolated from the fruits and other parts of P. longum.…”

Section: T Mentagrophytesmentioning

confidence: 99%

“…Forty species of Piper were reported from this region. Piper species are of great interest owing to their biological properties and a number of isolated bioactive molecules [11][12][13]. The local people use 10 species of Piper as insecticidal, larvicidal, fungicidal, carminative, antidote etc.…”

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confidence: 99%

Isolation and Characterization of Antidermatophytic Bioactive Molecules from Piper longum L. Leaves

et al. 2012

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Piper longum L. (Piperaceae) commonly known as ''long pepper'' is a well known medicinal plant in ayurveda. Different parts of this plant, such as root, seed, fruit, whole plant etc. are used traditionally in various ailments. Here we have investigated the antidermatophytic activity of sequentially extracted petroleum ether, chloroform, methanol and water extracts from P. longum leaf against Trichophyton mentagrophytes, T. rubrum, T. tonsurans, Microsporum fulvum and M. gypseum. Better activity of chloroform and methanol extracts was observed. The chloroform extract was selected for further study and the MIC value was recorded as 5.0 mg ml -1 against the test organisms. In the chloroform extract, tannins and phenolic compounds were detected. Further activity-guided fractionation of chloroform extract by silica gel column chromatography yielded nine major fractions. Among these, fraction-1, 4, 5 and 7 showed higher antidermatophytic activity. Fraction-4 on further purification by repeated column chromatography yielded a potential antidermatophytic fraction showing MIC value of 0.625 mg ml -1 against T. mentagrophytes and T. rubrum as determined by broth microdilution method. The major compounds were identified as 1,2-benzenedicarboxylic acid, bis(2-ethylhexyl) ester (C 24 H 38 O 4 ] (41.45 %), 2,2-dimethoxybutane (C 6 H 14 O 2 ] (13.6 %) and b-myrcene (C 10 H 16 ) (6.75 %) based on GC-MS data.

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“…The extract of P. betle has been reported to have anti-hypertensive activity while that of P. argyrophyllum considered as the inhibitor of aflatoxin B1-DNA binding [5]. The roots and fruits of P. chaba have numerous applications in medicine, particularly for asthma, bronchitis, fever, abdominal pain, and in hemorrhoidal afflictions [6]. P. longum has been used in traditional ayurvedic system of medicine against various physiological disorders including asthma [7].…”

Section: Introductionmentioning

confidence: 99%

“…P. longum has been used in traditional ayurvedic system of medicine against various physiological disorders including asthma [7]. Several other species of Piper are also used as important medicinal plants and mainly used for indigenous medication [6,8]. Piper is a model genus for research in the field of ecology and evolutionary biology [9].…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity Analysis in Piper Species (Piperaceae) Using RAPD Markers

Sen¹,

Skaria²,

Muneer

2010

Mol Biotechnol

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The genetic diversity of eight species of Piper (Piperaceae) viz., P. nigrum, P. longum, P. betle, P. chaba, P. argyrophyllum, P. trichostachyon, P. galeatum, and P. hymenophyllum from Kerala state, India were analyzed by Random amplified polymorphic DNA (RAPD). Out of 22 10-mer RAPD primers screened, 11 were selected for comparative analysis of different species of Piper. High genetic variations were found among different Piper species studied. Among the total of 149 RAPD fragments amplified, 12 bands (8.05%) were found monomorphic in eight species. The remaining 137 fragments were found polymorphic (91.95%). Species-specific bands were found in all eight species studied. The average gene diversity or heterozygosity (H) was 0.33 across all the species, genetic distances ranged from 0.21 to 0.69. The results of this study will facilitate germplasm identification, management, and conservation.

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Investigations toward new lead compounds from medicinally important plants

Cited by 30 publications

References 2 publications

Rapid structural determination of amides in Piper longum by high‐performance liquid chromatography combined with ion trap mass spectrometry

Rapid structural determination of amides in Piper longum by high‐performance liquid chromatography combined with ion trap mass spectrometry

Isolation and Characterization of Antidermatophytic Bioactive Molecules from Piper longum L. Leaves

Genetic Diversity Analysis in Piper Species (Piperaceae) Using RAPD Markers

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