Investigations on ultraviolet light and nitrous acid induced mutations of halotolerant bacterial strains for the treatment of tannery soak liquor

Sekar, Sudharshan; Sivaprakasam, Senthilkumar; Surianarayanan, M.

doi:10.1016/j.ibiod.2008.08.005

Cited by 11 publications

(9 citation statements)

References 16 publications

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“…These irradiations can induce mutations by forming pyrimidine dimerization and cross-links in DNA, but mutations can also be induced by exposure to various physical (Duan et al, 2015) or chemical agents (Sekar, Sivaprakasam, & Mahadevan, 2009). However, even if an induced mutation leads to enhanced degradation of various pollutants, such as phenol (Sekar et al, 2009), azo dye (Joshi, Inamdar, Jadhav, & Govindwar, 2013), tannery soak liquor (Sekar et al, 2009) and oil (Chen, Yang, Huang, Zhang, & Ding, 2011;Duan et al, 2015), the randomness of the approach makes it difficult to use it in bioremediation applications or in biodegradation testing strategies. Moreover, these types of mutation and genotype evolution induced by external stress are complex and difficult to predict in natural environments.…”

Section: Experimental Induction Of Adaptationmentioning

confidence: 99%

Implications of microbial adaptation for the assessment of environmental persistence of chemicals

Poursat

Spanning

Voogt

et al. 2019

Critical Reviews in Environmental Science and Technology

100

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Persistency of organic chemicals is a key property in their environmental risk assessment. Information on persistency is often derived from the results of biodegradability screening tests such as the ready biodegradability tests (RBTs). RBTs are, however, not designed for this purpose and suffer from several problems that lead to a high variability of the results and, hence, to difficulties in their interpretation. The origin and exposure history of the inocula used for biodegradability testing can lead to highly variable outcomes. Microbial adaptation to chemicals and its impact on biodegradation needs further investigation in order to have a better understanding of their effects on persistency assessments of chemicals. It is well described that microbial adaptation stimulates biodegradation of organic chemicals. Several mechanisms responsible for these phenomena have been described, amongst which are i) shifts in community composition or abundances, ii) mutations within populations, iii) horizontal gene transfer or iv) recombination events. These adaptation processes may well be mimicked under laboratory conditions, but the outcome remains difficult to predict as we lack a fundamental understanding of the adaptive responses. This review aims to bring together our current knowledge regarding microbial adaptation and its implication for the testing of biodegradation of chemicals. Abbreviations BHRBroad host range plasmid HGT

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Section: Experimental Induction Of Adaptationmentioning

confidence: 99%

Implications of microbial adaptation for the assessment of environmental persistence of chemicals

Poursat

Spanning

Voogt

et al. 2019

Critical Reviews in Environmental Science and Technology

100

View full text Add to dashboard Cite

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“…Some papers reported that UV mutagenesis has a positive effect on increasing the biodegradation ability of strains [ 24 , 35 ]. Yet, another study showed that UV mutagenesis had no effect on degradation efficiency [ 36 ]. The enhanced phenol-degrading efficiency of mutant strains may result from the increase in the activity of the relevant enzymes; we therefore also studied the kinetics of phenol degradation.…”

Section: Resultsmentioning

confidence: 99%

Enhancement of Phenol Biodegradation by Pseudochrobactrum sp. through Ultraviolet-Induced Mutation

Mao

Xin

2015

IJMS

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The phenol-degrading efficiency of Pseudochrobactrum sp. was enhanced by ultraviolet (UV) irradiation. First, a bacterial strain, Pseudochrobactrum sp. XF1, was isolated from the activated sludge in a coking plant. It was subjected to mutation by UV radiation for 120 s and a mutant strain with higher phenol-degrading efficiency, Pseudochrobactrum sp. XF1-UV, was selected. The mutant strain XF1-UV was capable of degrading 1800 mg/L phenol completely within 48 h and had higher tolerance to hydrogen ion concentration and temperature variation than the wild type. Haldane’s kinetic model was used to fit the exponential growth data and the following kinetic parameters were obtained: μmax = 0.092 h−1, Ks = 22.517 mg/L, and Ki = 1126.725 mg/L for XF1, whereas μmax = 0.110 h−1, Ks = 23.934 mg/L, and Ki = 1579.134 mg/L for XF1-UV. Both XF1 and XF1-UV degraded phenol through the ortho-pathway; but the phenol hydroxylase activity of XF1-UV1 was higher than that of XF1, therefore, the mutant strain biodegraded phenol faster. Taken together, our results suggest that Pseudochrobactrum sp. XF1-UV could be a promising candidate for bioremediation of phenol-containing wastewaters.

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“…The radiating range of UV is between 136 and 390 nm, among which 200 and 300 nm are effective for (Sekar et al 2009). In our experiment, the lethality of radiation time under a light of 254 nm was studied by accounting the survival cell numbers after 48 h' cultivation on nutrient agar plates (see Fig.…”

Section: Determination Of Suitable Uv Radiation Timementioning

confidence: 99%

“…There were 11 negative mutant strains and 4 positive mutant strains, which gave a degradation rate of 52.42, 51.05, 50.81, 48.98%, respectively, comparing with a degradation rate (41.83%) of the original one. It was reported that UV mutagenesis had no effect on degradation effectiveness of organic pollutants (Sekar et al 2009). However, the use of ultraviolet mutation was effective in selecting enhanced degrader and increasing viscous oil's degradation according to our experiment.…”

Section: Assay Of the Mutant Strainsmentioning

confidence: 99%

“…The selection and domestication of high effective strain is an important work leading to a high degradation of the pollutants. The gene sequence of the bacterial strain could be altered in order to induce its activity through mutation (Sekar et al 2009). Thus, ultraviolet mutation can accelerate the process of domesticating by obtaining some positive mutants.…”

Section: Introductionmentioning

confidence: 99%

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Enhanced bioremediation of soil contaminated with viscous oil through microbial consortium construction and ultraviolet mutation

Chen

Yang

Huang

et al. 2010

World J Microbiol Biotechnol

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This study focused on enhancing the bioremediation of soil contaminated with viscous oil by microorganisms and evaluating two strategies. Construction of microbial consortium and ultraviolet mutation were both effective applications in the remediation of soil contaminated with viscous oil. Results demonstrated that an interaction among the microorganisms existed and affected the biodegradation rate. Strains inoculated equally into the test showed the best remediation, and an optimal microbial consortium was achieved with a 7 days' degradation rate of 49.22%. On the other hand, the use of ultraviolet mutation increased one strain's degrading ability from 41.83 to 52.42% in 7 days. Gas chromatography and mass spectrum analysis showed that microbial consortium could treat more organic fractions of viscous oil, while ultraviolet mutation could be more effect on increasing one strain's degrading ability.

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Investigations on ultraviolet light and nitrous acid induced mutations of halotolerant bacterial strains for the treatment of tannery soak liquor

Cited by 11 publications

References 16 publications

Implications of microbial adaptation for the assessment of environmental persistence of chemicals

Implications of microbial adaptation for the assessment of environmental persistence of chemicals

Enhancement of Phenol Biodegradation by Pseudochrobactrum sp. through Ultraviolet-Induced Mutation

Enhanced bioremediation of soil contaminated with viscous oil through microbial consortium construction and ultraviolet mutation

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