Investigations on Blood Physiology, Tissues Histology and Gene Expression Profile of Fusarium oxysporum Challenged Fish

Abstract

Labeo rohita was challenged with Fusarium oxysporum to demonstrate a thorough immune response. Significant alterations in hematological parameters viz; erythrocyte, hemoglobin, hematocrit, mean corpuscular hemoglobin concentration, mean corpuscular volume, packed cell volume, platelets, and leukocytes were observed. Total serum protein, albumin, globulin, A/G ratio, aspartate aminotransferase, alkaline aminotransferase, alkaline phosphatase activity, and cholesterol were also altered in the infected fish. Marked histopathological lesions in gills, liver, and kidney (fusion of gill lamella, necrosis, edema, vacuolization, glomerulus congestion, dilated bowman's space and renal tubules alterations) were recorded. Tissue specific gene expression data showed that interleukin was upregulated in infected fish gills, liver, and kidney but was downregulated in blood. Tumor necrosis factor-α expression was upregulated in blood, liver, and kidney and was downregulated in gills. Upregulation of interferon-γ was observed in blood, gills, and kidney but downregulated in liver. All the cellular and molecular findings from this study can be taken as critical biomarkers in the advancement of diagnostic techniques against fungal infections as a part of wide-ranging programs of systematic and comprehensive monitoring of fish health and immune system.

Assessment of Dietary Supplementation of Lactobacillus rhamnosus Probiotic on Growth Performance and Disease Resistance in Oreochromis niloticus

“…For histological analysis, soft tissues from the gill, liver, and kidney were obtained. To prevent cellular autolysis, collected tissues were preserved in 10% buffered formalin for 24 h. Fixed tissues were dehydrated by exposing them to several grades of alcohol in ascending order for 15 to 20 min, and then they were preserved in absolute ethanol [ 29 ]. Following two rounds of 100% pure xylene immersion, tissues were subsequently embedded in paraffin wax.…”

A Comparative Analysis on the Innate Immune Responses of Cirrhinus mrigala Challenged with Pseudomonas aeruginosa and Fusarium oxysporum

“…Carp culture faces an alarming condition as a consequence of pathogenic challenges, resulting in an increased infection rate and mortalities due to loss of immune function [1]. Some of the key factors such as stress, contaminated diet, and unsanitary conditions contribute to an epidemic of bacterial infections in aquatic animals [2].…”