Investigation with Real-Time PCR and Histopathology on the presence of H. felis, H. heilmannii and H. pylori in dogs

Akçakavak, Gökhan; Tuzçu, Mehmet; Tuzcu, Nevin; ÇELİK, Zeynep; Tural, Aysenur; DAĞAR, Osman

doi:10.52973/rcfcv-e33214

Cited by 4 publications

(2 citation statements)

References 26 publications

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“…For this reason, non-invasive methods such as serology, culture or PCR, which can be made from blood, saliva and faecal samples, are preferred [6]. In addition, it was reported in a study that PCR analyses alone would not be sufficient to associate the presence of bacteria with the disease state in the diagnosis of Helicobacter, and this should be supported by histopathological analyses [7].…”

Section: Introductionmentioning

confidence: 99%

Treatment and Determination of the Presence of Helicobacter in Shelter dogs by Faecal Antigen Testing and Enzyme–Linked Immunosorbent Assay

Gökalp,

Kırbaş,

Sayar

et al. 2024

RC FCV-LUZ

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In order to determine the presence of Helicobacter in shelter Dogs in Yozgat Province of Türkiye by fecal antigen test and ELISA and to treat the infection during the initial period. As the material of the study, faecal antigen test, faecal samples for ELISA, blood samples for haematological and biochemical analyses were collected from 82 dogs in Sorgun animal shelter where food and water bowls were kept in compartments. Infected and control groups were formed according to the results of the fecal antigen test. As a result of fecal antigen test, 6 of 82 animals were antigen positive and 76 were antigen negative. According to ELISA analysis; 53 dogs were antibody positive and 29 dogs were antibody negative. No significance was determined between the groups in terms of age and gender in both tests (P>0.05). Dogs in the infected group were treated according to a protocol known as triple therapy, which is used in Helicobacter infections. WBC, NEU and MON counts, RDW and % NEU values of the infected group were higher in the hematological examination, while the % LYM values were significantly lower than the control group. In the biochemical examination, the concentrations TNF–α (21.17 pg·mL-1 vs. 48.21 pg·mL-1), IL–1β (73.41 pg·mL-1 vs. 37.60 pg·mL-1) (P<0.01) and CRP (644.0 mg·dL-1 vs. 234.01 mg·dL-1) (P<0.001) were found to be higher than those of the control group. As a result, it was determined in the study that the presence of Helicobacter was intense in dogs that were in one–to–one contact with each other, including the feeding of dogs in shelter conditions. With the methods used in the study, it is predicted that the presence of Helicobacter can be detected and treated in the early stages, thus preventing transmission of Helicobacter in dogs.

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Section: Introductionmentioning

confidence: 99%

Treatment and Determination of the Presence of Helicobacter in Shelter dogs by Faecal Antigen Testing and Enzyme–Linked Immunosorbent Assay

Gökalp,

Kırbaş,

Sayar

et al. 2024

RC FCV-LUZ

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“…The etiology of gastritis includes several agents and viruses that can induce mucosal damage in the stomach, especially Helicobacter pylori. If left untreated, acute gastritis can progress into chronic disease, leading to complications [1]. Gastritis constitutes one of the most common, lifelong, serious, and insidious diseases in humans.…”

Section: Introductionmentioning

confidence: 99%

The protective effect of Aspalathus linearis against the experimental gastric ulcer induced by ethanol in rats

Oruç,

Karakoç,

Ketani

et al. 2023

RC FCV-LUZ

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This study aimed to investigate the effect of Aspalathus linearis on the expression levels of major histocompatibility complex (MHC) molecules and their ability to present antigens to CD8, CD68, and T cells in an experimental rat model of gastritis. Furthermore, changes in tumor necrosis factor–alpha (TNF–α), myeloperoxidase, superoxide dismutase, and malondialdehyde levels due to the antioxidant properties of the plant were evaluated. The study included 24 female Wistar albino rats (Rattus norvegicus). A. linearis was administered to the treatment groups of an ethanol–induced gastritis rat model via oral gavage for 5 days the blood and stomach tissues were sampled for analysis. Biochemical evaluation revealed that TNF–α and myeloperoxidase levels decreased and superoxide dismutase activity increased in the A. linearis–treated groups compared with the ethanol–treated group. Histochemical evaluation revealed that the severity of inflammation decreased in the rats with gastritis treated with A. linearis. In addition, the number of cells expressing CD8 and CD68 decreased. The number of MHC–II immunopositive cells was higher in the A. linearis–treated group compared with the control and other treatment groups. A. linearis exhibits a protective effect on gastric mucosa by reducing the severity of oxidative stress owing to its antioxidant, anti–inflammatory, and antiapopitotic properties.

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Determination of Helicobacter heilmannii in cats by real time polymerase chain reaction and histopathology

TUZCU,

ÇELİK,

AKÇAKAVAK

et al. 2023

Veterinary Journal of Mehmet Akif Ersoy University

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Helicobacter species such as Helicobacter heilmannii, Helicobacter pylori, Helicobacter felis, Helicobacter bizzozeronii, Helicobacter salomonis have been identified in cats and dogs, and research suggesting that these species may be zoonotic agents have increased in recent years. The aim of this study is to reveal the presence of H. heilmannii in stomach and liver tissues taken during necropsies of domestic, stray and shelter cats by real-time PCR and histopathology and to compare histopathological findings and real-time PCR results. The material of the study consisted of stomach and liver tissues taken from 41 cats who died from different causes and were necropsied. DNA of H. heilmannii was determined in the stomach tissues of 36 cats and the liver tissues of 24 cats in the study conducted with real-time PCR using specific primers of H. heilmannii. Epithelial degeneration in the stomach, fibrosis and oedema in the lamina propria and lymphoplasmacytic cell infiltration were detected histopathologically in the cats diagnosed with gastritis. Eight cats were positive in Hematoxylin-Eosin staining and nineteen cats were positive in the staining with Warthin Starry of the sections, in terms of Helicobacter-like microorganisms. Microscopically, dissociation of the remark cords and hydropic degeneration in hepatocytes and mononuclear cell infiltrations in some sections were determined in the livers. In conclusion, with this study, it was understood that real-time PCR analyses are very useful in determining H. heilmannii in cats. However, histopathological examinations are necessary to associate the presence of bacteria with the development of the disease.

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Investigation with Real-Time PCR and Histopathology on the presence of H. felis, H. heilmannii and H. pylori in dogs

Cited by 4 publications

References 26 publications

Treatment and Determination of the Presence of Helicobacter in Shelter dogs by Faecal Antigen Testing and Enzyme–Linked Immunosorbent Assay

Treatment and Determination of the Presence of Helicobacter in Shelter dogs by Faecal Antigen Testing and Enzyme–Linked Immunosorbent Assay

The protective effect of Aspalathus linearis against the experimental gastric ulcer induced by ethanol in rats

Determination of Helicobacter heilmannii in cats by real time polymerase chain reaction and histopathology

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