Investigation on the insecticidal limonoid content of commercial biopesticides and neem extract using solid phase extraction

Abstract

Neem extract is an alternative insecticide for organic farming and is an allowed component for commercial biopesticide in Korea. However, crop protection properties are not consistent in commercial products. In this report, quantitative analysis of commercial biopesticides for the four insecticidal limonoids of neem extract-azadirachtin A, azadirachtin B, deacetylsalannin and salannin, was conducted through solid phase extraction method with lipophilic-hydrophilic balanced material. The recoveries of the four limonoids ranged from 80.5% to 105%, and their limit of quantitation ranged from 0.028 mg/L to 0.356 mg/L. On the five imported neem extracts, the total contents of the four bioactive limonoids extracted were from 321 mg/L to 5810 mg/L, but there were big variations in the relative composition of the limonoids. The total limonoidal concentrations in 23 commercial bio-pesticides made from neem showed from below LOQ to 7190 mg/L with significant differences in the relative composition. These differences determine the biopesticide's efficacy on pests, therefore, tracking the active ingredients is necessary for the quality control of commercial bio-pesticides.

Thermal Stability of Representative Bioactive Compounds in Biopesticide Derived from Castor Oil or Wormseed Extract under Controlled Temperature

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Quantitative Analysis of Matrine and Oxymatrine in Sophora flavescens Extract and Its Biopesticides by UPLC

“…Recently, Lee et al reported the analytical method using HLB SPE and UPLC for the quantitative analysis of bioactive limonoids in the biopesticides containing neem extract [33]. First, the HLB clean-up method was applied to the purification method for quantitation of alkaloidal matrines, which were spiked at a concentration of 10 mg·L −1 in the biopesticide containing neem extract.…”

“…The HLB clean-up procedure as reported by Lee et al was followed [33]. The HLB cartridge was first conditioned with 2 mL MeOH and 2 mL distilled water (DW), then the sample was loaded and washed with 5% aqueous MeOH (2 mL).…”

“…Although various clean-up procedures and instrumental analysis methods [26]- [32] for matrine and oxymatrine using liquid chromatography (LC), LC-mass spectroscopy (MS), and LC-MS/MS in Chinese medicines, animal tissues, and cell media had been reported, the quantitative analytical method for the content in biopesticides is still unknown. And the quantitative analysis of bioactive compounds in biopesticide can be more complicated than the extract due to some additives [33]. Therefore, the objective of this study was to compare the clean-up efficiency for commercial biopesticides containing S. flavescens extract with solid phase extraction (SPE), and to develop an ultra-performance liquid chromatography (UPLC) method for the simultaneous determination of matrine and oxymatrine.…”

Quantitative Analysis of Rotenone and Deguelin in Biopesticides Containing Derris Extract by Ultra performance Liquid Chromatography

“…최근 님, 고삼, 계피 및 마늘 추출물 함유 유기농자재 중 작물보호활성 성분을 지표성분으로 설정하고, hydrophilic liphophilic balance (HLB), ENVI-Carb 및 C18 카트리지를 이용한 고체상 추출법(solid phase extraction, SPE)과 ultra-performance liquid chromatography (UPLC) 및 GC 를 활용한 지표성분 분석법이 보고되었다 (Lee et al, 2013: Lim et al, 2014aLim et al, 2014b;Lim et al, 2014c …”