Investigation on the effects of the atmospheric pressure plasma on wound healing in diabetic rats

Fathollah, Sara; Mirpour, Shahriar; Mansouri, Parvin; Dehpour, Ahmad Reza; Ghoranneviss, M.; Rahimi, Nastaran; Naraghi, Z Safaei; Chalangari, Reza; Chalangari, Katalin Martits

doi:10.1038/srep19144

Cited by 99 publications

(84 citation statements)

References 30 publications

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“…These clinical experiences confirm a very early hypothesis in plasma medicine research, that plasma effects on wound healing may be a result of a two-step activity: antiseptics on wound surface in combination with stimulation of tissue regeneration (67) in several in vivo animal experiments (69,(74)(75)(76)(77)(78)(79)(80)(81)(82)(83)(84)(85)(86)(87)(88)(89)(90) and in human volunteers or patients with reasonably defined wounds (91)(92)(93). It has to be pointed out that these lastmentioned wound healing effects in vivo were demonstrated in acute wounds without any interfering microbial contamination.…”

Section: Biological Plasma Effects and Its Medical Use: Focus On Wounsupporting

confidence: 68%

Plasma Medicine: A Field of Applied Redox Biology

Schmidt

Bekeschus

et al. 2019

In Vivo

196

121

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Section: Biological Plasma Effects and Its Medical Use: Focus On Wounsupporting

confidence: 68%

Plasma Medicine: A Field of Applied Redox Biology

Schmidt

Bekeschus

et al. 2019

In Vivo

196

121

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“…TGFβ-2 has been associated with the increased production of inhibitory molecules, such as proteoglycans [39,40]. Controlled plasma treatment may affect the expression of TGF-β leading to decreased expression of the proteoglycans, thus, allowing regenerating axons to penetrate through the glial scar [41][42][43]. Additionally, FGF-2 and an inflammatory cytokine, interferon γ (IFNγ) are known to have a role in glial scar induction, with a direct increase in glial scarring correlated to increased levels of IFNγ [44] FGF-2 and IFNγ are believed to modulate one another after injury [45].…”

Section: Discussionmentioning

confidence: 99%

Non-Thermal Plasma Accelerates Astrocyte Regrowth and Neurite Regeneration Following Physical Trauma In Vitro

et al. 2019

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Non-thermal plasma (NTP), defined as a partially ionized gas, is an emerging technology with several biomedical applications, including tissue regeneration. In particular, NTP treatment has been shown to activate endogenous biological processes to promote cell regrowth, differentiation, and proliferation in multiple cell types. However, the effects of this therapy on nervous system regeneration have not yet been established. Accordingly, the current study explored the effects of a nanosecond-pulsed dielectric barrier discharge plasma on neural regeneration. Following mechanical trauma in vitro, plasma was applied either directly to (1) astrocytes alone, (2) neurons alone, or (3) neurons or astrocytes in a non-contact co-culture. Remarkably, we identified NTP treatment intensities that accelerated both neurite regeneration and astrocyte regrowth. In astrocyte cultures alone, an exposure of 20-90 mJ accelerated astrocyte re-growth up to three days post-injury, while neurons required lower treatment intensities (≤20 mJ) to achieve sub-lethal outgrowth. Following injury to neurons in non-contact co-culture with astrocytes, 20 mJ exposure of plasma to only neurons or astrocytes resulted in increased neurite regeneration at three days post-treatment compared to the untreated, but no enhancement was observed when both cell types were treated. At day seven, although regeneration further increased, NTP did not elicit a significant increase from the control. However, plasma exposure at higher intensities was found to be injurious, underscoring the need to optimize exposure levels. These results suggest that growth-promoting physiological responses may be elicited via properly calibrated NTP treatment to neurons and/or astrocytes. This could be exploited to accelerate neurite re-growth and modulate neuron-astrocyte interactions, thereby hastening nervous system regeneration.Each year in the US, more than 80,000 people are afflicted with severe traumatic brain injury (TBI), over 12,000 experience spinal cord injury (SCI), over 500,000 people suffer from stroke, and millions of people are diagnosed with chronic neurodegenerative diseases [2,3]. Following brain injury or neurodegenerative disease, successful regeneration often requires the reformation of long-distance communication fibers, referred to as axons. Long-distance axonal regeneration does not occur in the mature brain, and the formation of a glial scar, occurring after a severe injury, inhibits axon regeneration and cell migration [4]. Strategies to augment axonal regeneration and modulate the post-injury microenvironment are actively being investigated to promote healing and facilitate functional regeneration.Non-thermal plasma (NTP), sometimes referred to as cold atmospheric plasma, has been investigated to facilitate wound healing and promote tissue regeneration. It is a partially ionized gas that consists of several physical (e.g., electric fields, ultraviolet light) and chemical (e.g., radical species and charged and neutral molecules) constituents [5]. NTP has...

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“…Appropriate doses of cold plasma have antibacterial effects, activate fibroblast proliferation in wound tissue and thus promote wound healing in mice [35]. Interaction of ROS and RNS with wounded tissue accelerates repair processes without any adverse effects on normal tissue [36].…”

Section: Plasma and Wound Healing In Mammals And Amphibiansmentioning

confidence: 99%

Modulation of Metamorphic and Regenerative Events by Cold Atmospheric Pressure Plasma Exposure in Tadpoles, Xenopus laevis

et al. 2019

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Atmospheric pressure plasma has found wide clinical applications including wound healing, tissue regeneration, sterilization, and cancer treatment. Here, we have investigated its effect on developmental processes like metamorphosis and tail regeneration in tadpoles. Plasma exposure hastens the process of tail regeneration but delays metamorphic development. The observed differences in these two developmental processes following plasma exposure are indicative of physiological costs associated with developmental plasticity for their survival. Ultrastructural changes in epidermis and mitochondria in response to the stress of tail amputation and plasma exposure show characteristics of cellular hypoxia and oxidative stress. Mitochondria show morphological changes such as swelling with wide and fewer cristae and seem to undergo processes such as fission and fusion. Complex interactions between calcium, peroxisomes, mitochondria and their pore transition pathways are responsible for changes in mitochondrial structure and function, suggesting the subcellular site of action of plasma in this system.

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Investigation on the effects of the atmospheric pressure plasma on wound healing in diabetic rats

Cited by 99 publications

References 30 publications

Plasma Medicine: A Field of Applied Redox Biology

Plasma Medicine: A Field of Applied Redox Biology

Non-Thermal Plasma Accelerates Astrocyte Regrowth and Neurite Regeneration Following Physical Trauma In Vitro

Modulation of Metamorphic and Regenerative Events by Cold Atmospheric Pressure Plasma Exposure in Tadpoles, Xenopus laevis

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