Investigation on image processing parameters for plate evaluation in TLC analysis of mycotoxins

Abstract

ABSTRACT. Based on advantages of available modern image processing techniques, thin-layer chromatography combined with image analysis becomes a promising alternative for the mycotoxins determinations in various foodstuffs. For this, the effect of digitizing channel selection and image processing parameters on aflatoxin B2 (AB2) and ochratoxin A (OTA) determination was evaluated and the obtained results were compared with those of classical photodensitometric analysis. The best exciting wavelength for OTA was found to be λ excitation =333nm while for AB2 λ excitation =365nm. Low detection and quantification limits were determined (LOD OTA =0.310ng/spot; LOQ OTA =0.616ng/spot, LOD AB2 =0.557ng/spot, LOQ AB2 =1.098ng/spot). For image processing, the parameter with the highest influence upon the regression calibration curve was brightness and the best digitizing channel was found to be the green one. First-order calibration curve with a good determination coefficient (R 2 >0.99) were obtained on the working range of 2.5-50ng/spot. Although image processing techniques do not provide higher LOD and LOQ values than classical photodensitometry, this method offers the advantage of simultaneous quantification of both mycotoxins.

A Novel Fluorescent Aptasensor Based on Real-Time Fluorescence and Strand Displacement Amplification for the Detection of Ochratoxin A

“…In order to achieve the efficient detection of OTA in complex food matrices, various detection technologies have been developed. The commonly used analytical methods include high-performance liquid chromatography (HPLC) [ 10 ], liquid chromatography tandem mass spectrometry [ 11 ] and other chromatographic methods [ 12 , 13 ]. Although these methods are highly specific and accurate, there are still some problems such as time-consuming sample pre-treatment and professional operators of sophisticated instruments.…”

Functionalized Electrochemical Aptasensor for Sensing of Ochratoxin A in Cereals Supported byin SilicoAdsorption Studies

“…For this reason, as part of the established regulations for OTA in foodstuffs, the European Commission established the maximum OTA levels for various foods such as spices (15–20 μg kg –1 ), cereal products (3 μg kg –1 ), dried fruits (10 μg kg –1 ), soluble coffee (10 μg kg –1 ), baby foods (0.5 μg kg –1 ), and grape juices (2 μg kg –1 ). − Many different analytical techniques are available for the detection of OTA. They include high-performance liquid chromatography (HPLC) thin layer chromatography (TLC), liquid chromatography–mass spectroscopy (LC–MS), gas chromatography–mass spectroscopy (GC–MS), and enzyme-linked immunosorbent assays (ELISA) . Each of these generally acceptable methods has a limitation, such as low sensitivity, low limits of detection, and the need for skilled personnel with expertise to handle high-end instrumentation.…”

“…11−16 Many different analytical techniques are available for the detection of OTA. They include high-performance liquid chromatography (HPLC) 17 thin layer chromatography (TLC), 18 liquid chromatography− mass spectroscopy (LC−MS), 19 gas chromatography−mass spectroscopy (GC−MS), 20 and enzyme-linked immunosorbent assays (ELISA). 21 Each of these generally acceptable methods has a limitation, such as low sensitivity, low limits of detection, and the need for skilled personnel with expertise to handle high-end instrumentation.…”

Progress on Structured Biosensors for Monitoring Aflatoxin B1 From Biofilms: A Review

“…Traditionally, aflatoxin B1 (AFB1) detection is performed by thin-layer chromatography (TLC) (Var et al, 2007;Casoni et al, 2017), enzyme-linked immunosorbent assay (ELISA) (Lee et al, 2004), mass spectrometry (MS), gas chromatography (GC), liquid chromatography (LC) (Jin and Choi, 2007;Fan et al, 2015), and high-performance LC (HPLC) (Ghali et al, 2009). These detection assays benefit from having a high sensitivity and mature technology.…”