volume 189, issue 18, P6655-6664 2007
DOI: 10.1128/jb.00717-07
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Abstract: The SCO7222 protein and ActR are two of ϳ150 TetR-like transcription factors encoded in the Streptomyces coelicolor genome. Using bioluminescence as a readout, we have developed Escherichia coli-based biosensors that accurately report the regulatory activity of these proteins and used it to investigate their interactions with DNA and small-molecule ligands. We found that the SCO7222 protein and ActR repress the expression of their putative target genes, SCO7223 and actII-ORF2 (actA), respectively, by interact…

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