Investigation of the Biosynthetic Potential of Endophytes in Traditional Chinese Anticancer Herbs

Miller, Kristin; Chen, Qing; Sze, Daniel Man-Yuen; Neilan, Brett A.

doi:10.1371/journal.pone.0035953

Cited by 69 publications

(64 citation statements)

References 73 publications

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“…Although numerous endophytes have been isolated from traditional Chinese medicines (29), to the best of our knowledge, the present study is the first with regard to the endophytic microorganism isolated from the O. japonicas tuberous root ( Mai-dong ). The finding that endophytes mostly colonize the underground plant tissues, i.e., the root interior, was expected and is in agreement with previous studies (30,31).…”

Section: Resultsmentioning

confidence: 98%

Antitumor activity of bacterial exopolysaccharides from the endophyte Bacillus amyloliquefaciens sp. isolated from Ophiopogon japonicus

et al. 2013

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The endophytic bacterium, MD-b1, was isolated from the medicinal plant Ophiopogon japonicas and identified as the Bacillus amyloliquefaciens sp. with 99% similarity based on the partial sequence analysis of 16S rDNA. Exopolysaccharides were extracted from the endophyte for the evaluation of its antitumor activity against gastric carcinoma cell lines (MC-4 and SGC-7901). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays and microscopy were performed to estimate the cell viability and morphological changes of the MC-4 and SGC-7901 cells following treatment with the exopolysaccharides at 14, 22 and 30 μg/μl. The results revealed that the exopolysaccharides displayed concentration-dependent inhibitory effects against the MC-4 and SGC-7901 cells, with an IC50 of 19.7 and 26.8 μg/μl, respectively. The exopolysaccharides also induced morphological abnormalities in the cells. These effects indicated the the exopolysaccharides had an antitumoral mechanism of action associated with the mitochondrial dysfunction of the treated cells. This is the first study to investigate the endophytic microorganism isolated from O. japonicas and also the first discovery of such antitumoral exopolysaccharides derived from the genus Bacillus. This provides a promising and reproducible natural product source with high therapeutic value for anticancer treatment, thereby facilitating the development of new anticancer agents.

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Section: Resultsmentioning

confidence: 98%

Antitumor activity of bacterial exopolysaccharides from the endophyte Bacillus amyloliquefaciens sp. isolated from Ophiopogon japonicus

et al. 2013

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“…Before extracting the DNA of endophytes, the plants (leaf and root) were processed following the method as described by Miller [19]. The plants were surface sterilized by immersion in 30% hydrogen peroxide for 30 min, followed by rinsing in sterile Milli-Q filtered water (Millipore) (3 min × 3 times).…”

Section: Pretreatment Of Vegetable Samplesmentioning

confidence: 99%

Antibiotic resistance genes in manure-amended soil and vegetables at harvest

Wang

Qiao

Chen

et al. 2015

Journal of Hazardous Materials

258

106

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“…Thus, plants that host endophytes with great potential to produce polykletide and non-ribosomal peptidebased bioactive substances should be focused. (Miller et al, 2012).…”

Section: Mode Of Actionmentioning

confidence: 99%

Distribution and phytomedicinal aspects of Paris polyphylla Smith from the Eastern Himalayan Region: A review

Sharma¹,

Kalita²,

Tag³

2015

TANG [HUMANITAS MEDICINE]

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Comparative studies have established that the North-Eastern (NE) region of India which is a part of the Eastern Himalayan region is affluent in both traditional knowledge based phytomedicine and biodiversity. About 1953 ethno-medicinal plants are detailed from the NE region of India out of which 1400 species are employed both as food and ethnopharmacological resources. Nearly 70% of species diversity has been reported from the two Indian biodiversity hotspots-The Western Ghats and the Eastern Himalayas and these hotspots are protected by tribal communities and their ancient traditional knowledge system. Paris polyphylla Smith belongs to the family Melanthiaceae and is a traditional medicinal herb which is known to cure some major ailments such as different types of Cancer, Alzheimer's disease, abnormal uterine bleeding, leishmaniasis etc. The major phytoconstituents are dioscin, polyphyllin D, and balanitin 7. Phylogeny of Paris was inferred from nuclear ITS and plastid psbA-trnH and trnL-trnF DNA sequence data. Results indicated that Paris is monophyletic in all analyses. Rhizoma Paridis, which is the dried rhizome of Paris polyphylla is mainly used in Traditional Chinese Medicine and its mode of action is known for only a few cancer cell lines. The current review determines to sketch an extensive picture of the potency, diversity, distribution and efficacy of Paris polyphylla from the Eastern Himalayan region and the future validation of its phytotherapeutical and molecular attributes by recognizing the Intellectual Property Rights of the Traditional Knowledge holders.

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Investigation of the Biosynthetic Potential of Endophytes in Traditional Chinese Anticancer Herbs

Cited by 69 publications

References 73 publications

Antitumor activity of bacterial exopolysaccharides from the endophyte Bacillus amyloliquefaciens sp. isolated from Ophiopogon japonicus

Antitumor activity of bacterial exopolysaccharides from the endophyte Bacillus amyloliquefaciens sp. isolated from Ophiopogon japonicus

Antibiotic resistance genes in manure-amended soil and vegetables at harvest

Distribution and phytomedicinal aspects of Paris polyphylla Smith from the Eastern Himalayan Region: A review

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