Investigation of spectroscopic and proteomic alterations underlying prostate carcinogenesis

Felgueiras, Juliana; Silva, Joana Vieira; Nunes, Alexandra; Fernandes, Inês; Patrício, António; Maia, Nuno; Pelech, Steven; Fardilha, Margarida

doi:10.1016/j.jprot.2020.103888

Cited by 8 publications

(5 citation statements)

References 54 publications

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“…In the past decade, a number of studies identified potential therapeutic or prognostic targets by screening using transcriptomics, 5 proteomics, 6 or metabolomics 7 . However, the efficacy of the treatments requires additional improvement.…”

Section: Introductionmentioning

confidence: 99%

Vinculin orchestrates prostate cancer progression by regulating tumor cell invasion, migration, and proliferation

Zheng

Gong

et al. 2021

The Prostate

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Background: Prostate cancer (PCa) is a leading cause of death in men, and effective treatment of PCa requires further development. Our study aimed to investigate the potential role of vinculin (VCL) in PCa progression in vitro and in vivo. Methods: We investigated the methylation level of the VCL promoter based on the TCGA database. The knockdown efficacy of VCL gene expression was confirmed by quantitative polymerase chain reaction, Western blot analysis, and immunofluorescence. Furthermore, morphological changes in PCa cells were detected using phalloidin staining. The mobility of PCa cells was measured using transwell assays and high-content analysis. Moreover, cell growth and viability were determined using the colony formation and cell counting kit-8 assays. The role of VCL in tumor growth in vivo was investigated using a subcutaneous xenograft model generated by injecting tumor cells into the right flank of BALB/c nude mice. Results: The methylation level of the VCL promoter in PCa was significantly downregulated concomitant with age and the progression of nodal metastasis. VCL expression was markedly decreased by shRNA. Importantly, VCL knockdown significantly changed the cell morphology; inhibited the migration, invasion, and movement; and repressed colony formation and viability of PCa cells in vitro. Furthermore, downregulation of VCL suppressed tumor growth in vivo. Conclusions: Our study comprehensively evaluated the role of VCL in PCa progression in vivo and in vitro. The findings of the present study suggest that VCL can be a potential target for PCa prognosis and treatment.

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Section: Introductionmentioning

confidence: 99%

Vinculin orchestrates prostate cancer progression by regulating tumor cell invasion, migration, and proliferation

Zheng

Gong

et al. 2021

The Prostate

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“…Integrating proteomic and metabolomic data primarily focuses on profiling, pathway mapping, and association studies. For instance, distinguishing between PCa and normal prostate cells is achieved through a combination of proteomics and metabolomics, which enables the analysis of dysregulated lipid metabolism and increased protein phosphorylation [122]. The evolution of computing capabilities has allowed this integrated approach to move beyond simple pathway mapping [121].…”

Section: Integrated Metabolomics and Proteomicsmentioning

confidence: 99%

Metabolomics and Proteomics in Prostate Cancer Research: Overview, Analytical Techniques, Data Analysis, and Recent Clinical Applications

Al-Daffaie,

Al-Mudhafar,

Alhomsi

et al. 2024

IJMS

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Prostate cancer (PCa) is a significant global contributor to mortality, predominantly affecting males aged 65 and above. The field of omics has recently gained traction due to its capacity to provide profound insights into the biochemical mechanisms underlying conditions like prostate cancer. This involves the identification and quantification of low-molecular-weight metabolites and proteins acting as crucial biochemical signals for early detection, therapy assessment, and target identification. A spectrum of analytical methods is employed to discern and measure these molecules, revealing their altered biological pathways within diseased contexts. Metabolomics and proteomics generate refined data subjected to detailed statistical analysis through sophisticated software, yielding substantive insights. This review aims to underscore the major contributions of multi-omics to PCa research, covering its core principles, its role in tumor biology characterization, biomarker discovery, prognostic studies, various analytical technologies such as mass spectrometry and Nuclear Magnetic Resonance, data processing, and recent clinical applications made possible by an integrative “omics” approach. This approach seeks to address the challenges associated with current PCa treatments. Hence, our research endeavors to demonstrate the valuable applications of these potent tools in investigations, offering significant potential for understanding the complex biochemical environment of prostate cancer and advancing tailored therapeutic approaches for further development.

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“…A recent study investigated the possibility of exploring novel biomarkers through metabolic profiling of urine [ 168 , 169 ]. Indeed, metabolomics has been applied to different types of samples, including prostate tissue [ 170 ], cell lines [ 171 ], and serum [ 172 ], with the ultimate goal of finding novel diagnostic biomarkers. Regarding urine, guanidinoacetate, phenylacetylglycine, and glycine were appointed as potential candidate markers for PCa as their levels could be distinguished between PCa patients and healthy subjects.…”

Section: Identification Of Novel Biomarkers Including Lifestyle-assoc...mentioning

confidence: 99%

The Impact of Lifestyle on Prostate Cancer: A Road to the Discovery of New Biomarkers

Leitão

Matos

Roque

et al. 2022

JCM

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Prostate cancer (PCa) is one of the most common cancers among men, and its incidence has been rising through the years. Several risk factors have been associated with this disease and unhealthy lifestyles and inflammation were appointed as major contributors for PCa development, progression, and severity. Despite the advantages associated with the currently used diagnostic tools [prostate-specific antigen(PSA) serum levels and digital rectal examination (DRE)], the development of effective approaches for PCa diagnosis is still necessary. Finding lifestyle-associated proteins that may predict the development of PCa seems to be a promising strategy to improve PCa diagnosis. In this context, several biomarkers have been identified, including circulating biomarkers (CRP, insulin, C-peptide, TNFα-R2, adiponectin, IL-6, total PSA, free PSA, and p2PSA), urine biomarkers (PCA3, guanidine, phenylacetylglycine, and glycine), proteins expressed in exosomes (afamin, vitamin D-binding protein, and filamin A), and miRNAs expressed in prostate tissue (miRNA-21, miRNA-101, and miRNA-182). In conclusion, exploring the impact of lifestyle and inflammation on PCa development and progression may open doors to the identification of new biomarkers. The discovery of new PCa diagnostic biomarkers should contribute to reduce overdiagnosis and overtreatment.

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Investigation of spectroscopic and proteomic alterations underlying prostate carcinogenesis

Cited by 8 publications

References 54 publications

Vinculin orchestrates prostate cancer progression by regulating tumor cell invasion, migration, and proliferation

Vinculin orchestrates prostate cancer progression by regulating tumor cell invasion, migration, and proliferation

Metabolomics and Proteomics in Prostate Cancer Research: Overview, Analytical Techniques, Data Analysis, and Recent Clinical Applications

The Impact of Lifestyle on Prostate Cancer: A Road to the Discovery of New Biomarkers

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