Investigation of natural infection by Leishmania in sandflies of Paraná State, Southern Brazil

Scodro, Regiane Bertin de Lima; Reinhold-Castro, Kárin Rosi; Dias-Sversutti, Alessandra de Cassia; Neitzke-Abreu, Herintha Coeto; Membrive, Norberto; Kühl, João Balduíno; Silveira, Thaís Gomes Verzignassi; Teodoro, Ueslei

doi:10.1590/s1516-89132008000300007

Cited by 3 publications

(1 citation statement)

References 37 publications

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“…Focos da doença têm sido descritos em todos os continentes, à exceção da Oceania. Estima-se que existam 12 milhões de pessoas infectadas em todo o mundo, com aproximadamente 600.000 novos casos notificados por ano (Scodro, 2008). Segundo a OMS, globalmente, há uma estimativa anual de 70.000 mortes e 350 milhões de pessoas expostas ao risco de infecção e doença.…”

Section: Epidemiologiaunclassified

Epidemiologia da leishmaniose visceral humana em Araguaína (TO) e o diagnóstico sorológico da doença

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Human visceral leishmaniasis (HVL) is a severe systemic protozoonosis that has presented changes at the epidemiological behavior, with a growing trend in Brazil. It has been conducted a cross sectional study to assess the context of HVL in the city of Araguaina (TO) from January 2007 to August 2010. Additionally, tests were conducted to compare the diagnostic approaches for the serological diagnosis. In the distribution of cases of HVL in the districts of northern state of Tocantins, Araguaina represents 79.8% of the total cases in the period from 2007 to August 2010. The results of this study revealed that in this period, 769 cases were confirmed, while 98.7% are new cases. There was a male predominance of 57.8% and the most affected age group was 0-14 years, with 71.3% of reported cases. In the distribution by area of residence were reported 98.5% of cases in the urban area, showing the urbanization of the disease. In tests, we used the usual approaches network: an indirect immunofluorescence assay (IFA) and a conventional ELISA, plus a dissociative ELISA. ELISA assays showed high reproducibility. Positive patients with IFAT showed a frequency of 4% false-positive when tested in both ELISA. Among patients suspected there was a high frequency of positive to ELISA, the ELISA dissociative higher than in the conventional ELISA. In the asymptomatic population, there was a finding of positive samples by ELISA dissociative. The presence of immune complexes in asymptomatic patients early in the disease or its presence in patients with less intense humoral response, as children, these findings may explain the higher dissociative ELISA positive. Confirmation of these results depends on parasitologic research on dissociative ELISA-positive patients. These results show the importance of new approaches in the serological diagnosis LVH that would make a screening of patients for invasive diagnostic parasitology.

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Section: Epidemiologiaunclassified

Epidemiologia da leishmaniose visceral humana em Araguaína (TO) e o diagnóstico sorológico da doença

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Parasitological and molecular search for Leishmania natural infection in phlebotomine sand flies in peri-urban and rural sites of an Argentinean area endemic for tegumentary leishmaniasis

et al. 2021

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Natural Infection ofNyssomyia neivaibyLeishmania (Viannia) spp. in the State of Paraná, Southern Brazil, Detected by Multiplex Polymerase Chain Reaction

Oliveira

Reinhold-Castro

Bernal

et al. 2011

Vector-Borne and Zoonotic Diseases

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Natural sandfly infection by Leishmania spp. in an area endemic for American cutaneous leishmaniasis was analyzed using multiplex polymerase chain reaction (PCR). The sandflies were captured using Falcão light traps in an endemic area of the municipality of Doutor Camargo during March, April, and June 2008. In total, 1803 females were analyzed; 1755 were Nyssomyia neivai (Pinto) and 48 were Nyssomyia whitmani (Antunes and Coutinho). Multiplex PCR analyses using MP3H-MP1L and 5Llcac-3Llcac primers showed the presence of Leishmania (Viannia) spp. in 4/181 pools of sandflies, all Ny. neivai, that is, a minimal infection rate of 0.22%. This study showed, for the first time, the presence of DNA of Leishmania (Viannia) spp. in Ny. neivai. This suggests the existence of natural infection by Leishmania (Viannia) spp. in Ny. neivai in the state of Paraná. Multiplex PCR is an important tool in the detection of Leishmania infection in sandflies.

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Investigation of natural infection by Leishmania in sandflies of Paraná State, Southern Brazil

Abstract: The purpose of this work was to verify the occurrence of Leishmania in naturally infected

Cited by 3 publications

References 37 publications

Epidemiologia da leishmaniose visceral humana em Araguaína (TO) e o diagnóstico sorológico da doença

Epidemiologia da leishmaniose visceral humana em Araguaína (TO) e o diagnóstico sorológico da doença

Parasitological and molecular search for Leishmania natural infection in phlebotomine sand flies in peri-urban and rural sites of an Argentinean area endemic for tegumentary leishmaniasis

Natural Infection ofNyssomyia neivaibyLeishmania (Viannia) spp. in the State of Paraná, Southern Brazil, Detected by Multiplex Polymerase Chain Reaction

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