In this work, a novel S-substituted bioactive anthraquinone compound is synthesized with a new, easy and less energetic reaction methodfrom 1-chloro-9,10-dihydrodiagnosisxy-anthraquinone and butyl-3-mercaptopropionate (Compound 3) for the first time in the literature. The synthesized structure is purified by column chromatography and then characterized with various spectroscopic methods (NMR, MS, FT-IR, UV). The investigation of the antimicrobial properties of the purified structure reveals remarkable biological properties. Compound 3 is not only effective against yeasts and fungi but also displays significant inhibitory effects on the growth of the tested Gram positive bacteria similiar to that of a positive control (Gentamicin). Subsequently, biodegradable electrospun mats are produced via electrospinning method for their usage as a biomaterial. Structural (FTIR), morphological (FEG-SEM) biological (antimicrobial and in-vitro tests) and mechanical (tensile testing) characterizations are conducted for these nanobiomaterials. Presenting an advantage of the novel antimicrobial compound, all produced electrospun nanobiocomposites exhibit remarkable cell viability% and mechanical properties as the amount of Compound 3 increases. Cell viability values are 95% or greater for all polymeric nanocomposites whereas, the best cell viability% and mechanical proprties are obtained for PCL-8% Compound3 composite. The obtained electrospun mats are good candiates for biomaterials for tissue engineering purposes and wound healing materials with a purposeful compound synthesis and a subsequent nanobiocomposite production.