Investigation of miR-93-5p and its effect on the radiosensitivity of breast cancer

Abstract

Accumulating evidence suggests that intrinsic resistance to radiotherapy reduces the survival of patients with cancer. The present study investigated whether miR-93-5p affects proliferation, migration, apoptosis, and radiosensitivity of breast cancer (BC) cells. MDA-MB-468, MCF-7, and MDA-MB-231 BC cells were incubated with hsa-miR-93-5p mimics, hsa-miR-93-5p inhibitor, and negative control RNA with or without exposure to ionizing radiation to determine cell proliferation, migration, and apoptosis using the Cell Counting Kit-8 assay, wound healing assay and apoptotic assay, respectively. Overexpression of miR-93-5p inhibited the migratory abilities (P = 0.001) and decreased the cell proliferation (P = 0.049) of MCF-7 cells. In MCF-7 cells, a significant increase in apoptosis was detected after treatment with miR-93-5p compared with the negative control (P = 0.001) and miR-93-5p inhibitor (P = 0.004). In MDA-MB-468 cells, the proportion of apoptotic cells increased following exposure to ionizing radiation (P = 0.001). The percentage of apoptotic MDA-MB-231 cells in the miR-93-5p group was significantly increase compared with that determined in the negative control (P = 0.044) and hsa-miR-93-5p inhibitor (P = 0.046) groups. In conclusion, our findings showed that miR-93-5p reduces BC cell proliferation and migratory capacity, and increases the ratio of apoptotic cells. Overexpression of miR-93-5p could increase radiosensitivity in BC cells by increasing apoptosis. This evidence provides new insight into the treatment of BC and identifies miR-93-5p as a potential therapeutic target.

Correlation between Platelet miRNA Expression and Coagulation Function in Children with Severe Pneumonia

“…miRNA regulates most of the genes and employed in various cellular processes such as cell proliferation, differentiation, metabolism, and apoptosis [ 13 ]. Currently, miRNA has been used as biomarker for disease diagnosis in many clinical fields, such as cardiovascular and cerebrovascular diseases, autoimmune diseases, and tumors [ 14 , 15 ]. Studies have shown that miR-223 is highly expressed in peripheral blood of SP patients and can be used as an early diagnostic marker for severe pneumonia [ 16 ].…”

Upregulation of circ_0008812 and circ_0001583 predicts poor prognosis and promotes breast cancer proliferation

“…After intersecting with 289 DEmiRNAs, 25 miRNAs were obtained. Among them, 7 miRNAs (miR-671-5p, miR-940, miR-124-3p, miR-944, miR-93-5p, miR-512-3p and miR-142-5p) have been reported in breast cancer ( Flores-Pérez et al, 2016 ; Tan et al, 2016 ; Wang et al, 2016 ; Zhang et al, 2020a ; Elango et al, 2020 ; Mohamadzade et al, 2021 ; Pan et al, 2021 ). For instance, overexpression of miR-671-5p suppressed FOXM1 expression, thereby reducing breast cancer cells growth and invasion ( Tan et al, 2016 ).…”

“…MiR-124-3p could inhibit the proliferation of MCF-7 and MDA-MB-231 cells by suppressing CBL expression ( Wang et al, 2016 ). Pan et al (2021) showed that an increase in miR-93-5p could radiosensitize breast cancer cells by increasing apoptosis. Furthermore, we obtained 1,148 mRNAs targeted by these 25 miRNAs based on three online databases.…”

ADGRD1 as a Potential Prognostic and Immunological Biomarker in Non-Small-Cell Lung Cancer

“…miR-142-5p overexpression may increase breast cancer proliferation, invasion, and migration when SORBS1 is targeted [ 37 ]. Increased apoptosis and decreased recurrence rate are associated with increased radio-sensitivity of breast cancer cells when miR-93-5p is overexpressed, implying the role of miR-93-5p may act as a potential therapeutic target for breast cancer [ 38 ]. miR-17-5p may have an important effect on pathogenesis of triple-negative breast cancer, and its increased expression is associated with prognosis, implying the role of miR-17-5p as the therapeutic target for triple-negative breast cancer [ 39 ].…”