Investigation of interactions between dendrimer-coated magnetite nanoparticles and bovine serum albumin

Pan, Bifeng; Gao, Feng; Ao, Limei

doi:10.1016/j.jmmm.2005.02.018

Cited by 113 publications

(46 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Dendrimer should become one kind of good nonviral synthetic vectors because of the advantages of safety, simplicity of use, and ease of mass production compared with viral vectors with an inherent risk for clinical use (24,25). Nanoparticles coated with dendrimer can alter the charge, functionality, and reactivity and enhance the stability and dispersibility of the nanoparticles (27)(28)(29).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Dendrimer-Modified Magnetic Nanoparticles Enhance Efficiency of Gene Delivery System

Pan

Cui²,

Sheng³

et al. 2007

Cancer Research

Self Cite

295

165

View full text Add to dashboard Cite

Magnetic nanoparticles (MNP) with a diameter of 8 nm were modified with different generations of polyamidoamine (PAMAM) dendrimers and mixed with antisense survivin oligodeoxynucleotide (asODN). The MNP then formed asODNdendrimer-MNP composites, which we incubated with human tumor cell lines such as human breast cancer MCF-7, MDA-MB-435, and liver cancer HepG2 and then analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, quantitative reverse transcription-PCR, Western blotting, laser confocal microscopy, and high-resolution transmission electron microscopy. Results showed that the asODN-dendrimer-MNP composites were successfully synthesized, can enter into tumor cells within 15 min, caused marked down-regulation of the survivin gene and protein, and inhibited cell growth in dose-and time-dependent means. No.5 generation of asODN-dendrimer-MNP composites exhibits the highest efficiency for cellular transfection and inhibition. These results show that PAMAM dendrimer-modified MNPs may be a good gene delivery system and have potential applications in cancer therapy and molecular imaging diagnosis.

show abstract

Section: Introductionmentioning

confidence: 99%

“…MNPs were prepared under various reaction conditions by coprecipitation of Fe 2+ and Fe 3+ in the presence of NaOH (27,28). Finally, MNPs were dispersed in PBS (pH 7.4).…”

Section: Introductionmentioning

confidence: 99%

Dendrimer-Modified Magnetic Nanoparticles Enhance Efficiency of Gene Delivery System

Pan

Cui²,

Sheng³

et al. 2007

Cancer Research

Self Cite

295

165

View full text Add to dashboard Cite

show abstract

“…This fluorescence is mainly attributed to the presence of Trp and Tyr residues [17,18]. The interaction between BSA and WSCP was investigated by evaluating fluorescence intensity on the BSA before and after the addition of WSCP.…”

Section: Resultsmentioning

confidence: 99%

Study on the Interaction between BSA and a Water-Soluble Cationic Poly(heteroarylene methine) Bearing Pyridinium Salts

Sakamaki

Kato

Fukushima

2013

J. Photopol. Sci. Technol.

View full text Add to dashboard Cite

The interaction of BSA and a water-soluble cationic poly(heteroarylene methine) bearing pyridinium salts (WSCP) has been investigated by spectrophotometric and spectrofluorometric methods. The absorption and fluorescence spectra of BSA suggest that BSA and WSCP form a protein-polymer ground state complex due to electrostatic interaction between the negatively charged surface of BSA and the cationic side chains of WSCP. The interaction causes a decrease in fluorescence intensity of BSA due to static quenching. In addition, an enhancement in fluorescence signal of WSCP with BSA can be attributed to the surfactant property of BSA. The complex formation between BSA and WSCP results from not only electrostatic attractive force but also hydrophobic interaction, and WSCP can become a fluorescence probe for homogeneous BSA detection.

show abstract

“…MNPs were prepared by coprecipitation of Fe 2+ and Fe 3+ in the presence of NaOH, 21,22 and were dispersed in phosphate buffered saline (PBS) (pH 7.4). G5.0 PAMAM dMNPs were prepared according to our previous reports.…”

Section: Preparation and Characterization Of Dmnpsmentioning

confidence: 99%

Efficient preparation and labeling of human induced pluripotent stem cells by nanotechnology

Ruan¹,

Shen²,

Wang³

et al. 2011

IJN

View full text Add to dashboard Cite

Efficient preparation and labeling of human induced pluripotent stem (iPS) cells is a great challenge in stem cell research and development. With the aim of investigating the feasibility of using nanotechnology to enhance the preparation efficiency of iPS cells and to label iPS cells for long-term tracing and imaging, in this paper, four transcription factor genes, ie, Oct4, Sox2, LIN28, and Nanog, and packaging plasmids such as PSPAX2 and PMD2.G were cotransfected into 293T cells using Generation 5.0 polyamidoamine dendrimer-modified magnetic nanoparticles (dMNPs) as a delivery system. The resultant supernatant liquids were incubated with human fibroblast cells at 37°C for 21 days, then the embryonic stem (ES) cell-like clones were screened, cultured, and identified. Finally, the prepared iPS cells were labeled with fluorescent magnetic nanoparticles (FMNPs). The results showed that dMNPs can efficiently deliver all vectors into 293T cells. The resultant lentiviruses’ titers were 10-fold more than those based on Lipofectamine™ 2000. Reverse transcription polymerase chain reaction analysis showed that four genes (Oct4, Sox2, LIN28, and Nanog) exhibited different expressions in iPS cells. Immunostaining analysis showed that specific surface markers of ES cells such as SSEA-3, SSEA-4, Tra-1-60, and Tra-1-81 were positive in iPS cells, and the terotomas were formed in NOD-SCID mice that were implanted with iPS cells. Red fluorescent signals could be observed in iPS cells labeled with FMNPs by fluorescent microscopy, and the magnetic signals were detected in labeled iPS cells by magnetic resonance imaging. In conclusion, human iPS cells can be efficiently generated using polyamidoamine dMNPs and lentivirus and labeled with FMNPs for long-term observation and tracking, which has great potential application in the research and development of stem cells in the near future.

show abstract

Investigation of interactions between dendrimer-coated magnetite nanoparticles and bovine serum albumin

Cited by 113 publications

References 11 publications

Dendrimer-Modified Magnetic Nanoparticles Enhance Efficiency of Gene Delivery System

Dendrimer-Modified Magnetic Nanoparticles Enhance Efficiency of Gene Delivery System

Study on the Interaction between BSA and a Water-Soluble Cationic Poly(heteroarylene methine) Bearing Pyridinium Salts

Efficient preparation and labeling of human induced pluripotent stem cells by nanotechnology

Contact Info

Product

Resources

About