Investigation of Fragment Antibody Stability and Its Release Mechanism from Poly(Lactide-co-Glycolide)-Triacetin Depots for Sustained-Release Applications

Chang, Debby P.; Garripelli, Vivek Kumar; Rea, Jennifer C.; Kelley, Robert F.; Rajagopal, Karthikan

doi:10.1002/jps.24546

Cited by 25 publications

(28 citation statements)

References 39 publications

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“…PEG is a plasticizer and TA is a solvent of PLA (Chang and Garripelli 2015). As a result, TA separates PLA particles from each other.…”

Section: Morphological Studymentioning

confidence: 99%

“…In samples containing PLA and TA, the material structure is not integrated and microsized particles are separated from each other. Triacetin is a relatively less hydrophobic solvent (50 mg/mL aqueous phase solubility), but can dissolve a hydrophobic polymer such as PLA (Chang and Garripelli 2015). TA is a triester and PLA is a polyester.…”

Section: Morphological Studymentioning

confidence: 99%

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Improvement in mechanical properties and biodegradability of PLA using poly(ethylene glycol) and triacetin for antibacterial wound dressing applications

2020

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Wound is among the most common injuries. A suitable wound dressing has a significant effect on the healing process. In this study, a porous wound dressing was prepared using poly (lactic acid) (PLA) and two plasticizers, polyethylene glycol (PEG) and triacetin (TA), through solvent casting method. For antibacterial activities, metronidazole was incorporated in the structure. The morphology was investigated by scanning electron microscopy (SEM). In addition, the effect of plasticizers ratio on porosity growth was evaluated. It was also observed that each had a unique effect on the structure's porosity. The mechanical properties confirmed the effect of both plasticizers on increasing polymer softness and flexibility, and the most similar formulations to human skin in terms of mechanical properties were introduced. According to the results, TA had stronger effect on mechanical properties. The differential scanning calorimetry (DSC) showed the effect of increasing plasticizer concentration on crystalline structure and T m reduction of PLA. The water contact angle measurement showed that both plasticizers enhanced hydrophilic characteristics of PLA, and this effect was weaker in PEG-containing formulations. The in vitro degradation study showed biodegradability, as a desirable property in wound dressing. Results suggested that higher degradation can be obtained by both plasticizers at the same time. The results also showed that PEG was more effective in enhancing water absorbency. In vitro drug release study indicated an explosive release and the highest amount was 85% over 186 h. The antibacterial activity test confirmed the effectiveness of the drug in preventing bacterial growth in the drug-containing formulations, while it showed the antibacterial property of TA. MTT assay was performed and the cellular toxicity of the formulations was checked and those that revealed the least toxicity were introduced.

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“…PEG is a plasticizer and TA is a solvent of PLA (Chang and Garripelli 2015). As a result, TA separates PLA particles from each other.…”

Section: Morphological Studymentioning

confidence: 99%

Section: Morphological Studymentioning

confidence: 99%

Improvement in mechanical properties and biodegradability of PLA using poly(ethylene glycol) and triacetin for antibacterial wound dressing applications

2020

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“…[2][3][4][5][6] More recently, PLGA implants (Surodex; Oculex Pharmaceuticals, Inc., Sunnyvale, CA, USA, and Ozurdex; Allergan plc, Parsippany, NJ, USA) have been used to successfully deliver steroids to the eye over the course of weeks to months, thereby reducing the frequency of intravitreal injections. 7,8 Importantly, recent advances in protein processing techniques and formulation development have enabled the preparation of protein-loaded PLGA rods 9 and PLGA-solvent depots 10 for sustained-release applications.…”

mentioning

confidence: 99%

Evaluation of the Toxicity of Intravitreally Injected PLGA Microspheres and Rods in Monkeys and Rabbits: Effects of Depot Size on Inflammatory Response

Thackaberry¹,

Farman²,

Zhong³

et al. 2017

Invest. Ophthalmol. Vis. Sci.

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“…Triacetin is a pharmaceutically important chemical substance used as a solvent 11 for the solubilization of various drugs and polymers because of its biocompatibility in topical and injectable preparations. 12,13 It is also capable of affecting the film-forming properties 14 as well as adhesive properties in topical preparations and is capable of forming stable depots in the case of injectable preparations. 15 Thus, the present work was carried out to establish the suitability of EHSA to study the solute-solvent interaction and solution behavior and to predict the solubility of ITRA in triacetin: water mixtures as a function of the solubility parameter.…”

Section: Introductionmentioning

confidence: 99%

Extended Hildebrand Solubility Approach: Prediction and Correlation of the Solubility of Itraconazole in Triacetin: Water Mixtures at 298.15°K

Jagdale¹,

Nawale

2020

tjps

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Amaç: Triasetin: su karışımlarında kristal formdaki itrakonazol (ITRA)'nın çözünürlüğünün genişletilmiş Hildebrand çözünürlük yaklaşımı (EHSA) için uygunluğunun deneysel bir yaklaşımla tahmin ve korele edilmesi bu araştırmanın amacıdır. Gereç ve Yöntemler: ITRA'nın füzyon entalpisi, Hildebrand çözünürlük yaklaşımı çözünürlük parametresi ve ideal mol oranı gibi fizikokimyasal özellikleri tahmin edilmiştir. ITRA'nın triastin: sudan oluşan karışım halindeki çözeltilerdeki çözünürlükleri 298,15°K'da belirlenmiştir. Teorik çözünürlükleri çözelti karışımındaki çözünürlük parametresi (δ 1)'nin bir fonksiyonu olarak etkileşim enerji parametresi W kullanılarak polinominal regresyon denklemi ile hesaplanmıştır. Bulgular: Tüm triasetin: su karışımlarında ITRA'nın çözünürlüğü atmıştır ve çözünürlüğün en yüksek olduğu karışım ITRA'nın çözünürlük parametresinin çözelti karışımınınkine eşit olduğu karışımdır. Doğrudan yöntemin tahmin kapasitesi (ortalama % sapması-%1,89) beşinci polinominal sırada EHSA'dan (ortalama % sapması %9,76) daha iyi bulunmuştur. Sonuç: Bu sonuçlar çözünen kristalin çözünürlüğünün tek başına fizikokimyasal özellikler ve EHSA bilgileriyle yeterince ögörülebileceğini ve ilişkilendirilebileceğini göstermiştir. Bu bilgi süreç ve formülasyon geliştirmede yardımcı olabilir.

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Investigation of Fragment Antibody Stability and Its Release Mechanism from Poly(Lactide-co-Glycolide)-Triacetin Depots for Sustained-Release Applications

Cited by 25 publications

References 39 publications

Improvement in mechanical properties and biodegradability of PLA using poly(ethylene glycol) and triacetin for antibacterial wound dressing applications

Improvement in mechanical properties and biodegradability of PLA using poly(ethylene glycol) and triacetin for antibacterial wound dressing applications

Evaluation of the Toxicity of Intravitreally Injected PLGA Microspheres and Rods in Monkeys and Rabbits: Effects of Depot Size on Inflammatory Response

Extended Hildebrand Solubility Approach: Prediction and Correlation of the Solubility of Itraconazole in Triacetin: Water Mixtures at 298.15°K

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