Investigation of Cd(II), Pb(II) and Cu(I) complexation by glutathione and its component amino acids by ESI-MS and size exclusion chromatography coupled to ICP-MS and ESI-MS

Pawlak, Katarzyna; Ruzik, Lena; Lipiec, Elżbieta

doi:10.1016/j.talanta.2007.02.008

Cited by 48 publications

(36 citation statements)

References 21 publications

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“…Reduced sulfur in the form of thiol groups (-SH) is present in the cysteine residues of the important peptide glutathione (GSH) and the metal-induced binding phytochelatins (PCs) and metallothioneins. All of the aforementioned sulfur rich compounds are used by plants to bioinactivate heavy metals such as Cd [2,24,25]. In the present study, an increase in S concentration was found to correlate with increasing concentration of Cd within roots (Table 1, Fig.…”

Section: Effect Of Cadmium On Nutrients Uptakesupporting

confidence: 60%

Coordination and speciation of cadmium in corn seedlings and its effects on macro- and micronutrients uptake

Castillo‐Michel

Hernandez

Martínez‐Martínez

et al. 2009

Plant Physiology and Biochemistry

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Section: Effect Of Cadmium On Nutrients Uptakesupporting

confidence: 60%

Coordination and speciation of cadmium in corn seedlings and its effects on macro- and micronutrients uptake

Castillo‐Michel

Hernandez

Martínez‐Martínez

et al. 2009

Plant Physiology and Biochemistry

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“…However, the most preferred geometry for Pb(II) in a thiol rich environment is trigonal pyramidal with a lone pair occupying the apical position (hemidirected) [17]. Recently Polec-Pawlak and coworkers have used size exclusion chromatography coupled to ICP-MS and ESI to probe the speciation of Pb(II) ions with glutathione [25]. A variety of species were observed including [Pb(GS) 3 ] − .…”

Section: Resultsmentioning

confidence: 99%

Pb-207 NMR spectroscopy reveals that Pb(II) coordinates with glutathione (GSH) and tris cysteine zinc finger proteins in a PbS3 coordination environment

Neupane

Pecoraro

2011

Journal of Inorganic Biochemistry

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207Pb NMR spectroscopy can be used to monitor the binding of Pb(II) to thiol rich biological small molecules such as glutathione and to zinc finger proteins. The UV/visible (UV/Vis) absorption band centered at 334 nM and the observed 207Pb-signal in 207Pb NMR (δ ~ 5750 ppm) indicate that glutathione binds Pb(II) in a trigonal pyramidal geometry (PbS3) at pH 7.5 or higher with a 1:3 molar ratio of Pb(II) to GSH. While previous studies using UV/Vis and extended X-ray absorption fine structure (EXAFS) spectroscopy were interpreted to show that the zinc binding domain from HIV nucleocapsid protein (HIV-CCHC) binds Pb(II) in a single PbS3 environment, the more sensitive 207Pb NMR spectra (at pH 7.0, 1:1 molar ratio) provide compelling evidence for the presence of two PbS3 structures (δ = 5790 and 5744 ppm), one of which is more stable at high temperatures. It has previously been proposed that the HIV-CCHH peptide does not fold properly to afford a PbS2N motif, because histidine does not bind to Pb(II). These predictions are confirmed by the present studies. These results demonstrate the applicability of 207Pb NMR to biomolecular structure determination in proteins with cysteine binding sites for the first time.

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“…Different experimental techniques have been devoted to investigate the metal complexes of GSH, such as electron paramagnetic resonance (EPR) [26,27], NMR [28,29], X-ray diffraction (X-ray) [30,31] and ESI-MS [32]. Nevertheless, it is difficult to explain the structures of the complexes at the atomic and electronic level.…”

Section: Introductionmentioning

confidence: 99%

Probing the coordination properties of glutathione with transition metal ions (Cr2+,Mn2+,Fe2+,Co2+, Ni2+,Cu2+,Zn2+,Cd2+,Hg2+) by density functional theory

Liu

et al. 2014

J Biol Phys

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Probing the coordination properties of glutathione with transition metal ions (Cr 2+,were larger than that of M-N and M-O; for Cr 2+ complexes, most of the WBIs of M-O in complexes were higher than that of M-S and M-N. Furthermore, the changes in the electron configuration of the metal cations before and after chelate reaction revealed that Cu 2+ , Ni 2+ , Co 2+ and Hg 2+ had obvious tendencies to be reduced to Cu + , Ni + , Co + and Hg + during the coordination process.

show abstract

Investigation of Cd(II), Pb(II) and Cu(I) complexation by glutathione and its component amino acids by ESI-MS and size exclusion chromatography coupled to ICP-MS and ESI-MS

Cited by 48 publications

References 21 publications

Coordination and speciation of cadmium in corn seedlings and its effects on macro- and micronutrients uptake

Coordination and speciation of cadmium in corn seedlings and its effects on macro- and micronutrients uptake

Pb-207 NMR spectroscopy reveals that Pb(II) coordinates with glutathione (GSH) and tris cysteine zinc finger proteins in a PbS3 coordination environment

Probing the coordination properties of glutathione with transition metal ions (Cr2+,Mn2+,Fe2+,Co2+, Ni2+,Cu2+,Zn2+,Cd2+,Hg2+) by density functional theory

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