Investigation of avian influenza virus in poultry and wild birds due to novel avian-origin influenza A(H10N8) in Nanchang City, China

Ni, Xiansheng; He, Fenglan; Hu, Maohong; Zhou, Xianfeng; Wang, Bin; Feng, Changhua; Wu, Yumei; Li, Youxing; Tu, Junling; Li, Hui; Liu, Mingbin; Chen, Haiying; Chen, Shengen

doi:10.1016/j.micinf.2014.09.007

Cited by 19 publications

(12 citation statements)

References 29 publications

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“…Neu5Ac␤2-6Gal␤1-4GlcNAc␤ NB NB NB NB detected in Jiangxi Province, and evidence suggests a zoonotic source, as all three patients had visited live bird markets where the virus had been detected in poultry (27,28). Although two human infections with A(H10N7) subtypes had been reported, with patients presenting with conjunctivitis or mild respiratory symptoms (29,30), this was the first reported human infection with an influenza virus with an N8 subtype NA.…”

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confidence: 81%

Structure and Receptor Binding Preferences of Recombinant Hemagglutinins from Avian and Human H6 and H10 Influenza A Virus Subtypes

Yang

Carney

Chang

et al. 2015

J Virol

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During 2013, three new avian influenza A virus subtypes, A(H7N9), A(H6N1), and A(H10N8), resulted in human infections.While the A(H7N9) virus resulted in a significant epidemic in China across 19 provinces and municipalities, both A(H6N1) and A(H10N8) viruses resulted in only a few human infections. This study focuses on the major surface glycoprotein hemagglutinins from both of these novel human viruses. The detailed structural and glycan microarray analyses presented here highlight the idea that both A(H6N1) and A(H10N8) virus hemagglutinins retain a strong avian receptor binding preference and thus currently pose a low risk for sustained human infections. IMPORTANCEHuman infections with zoonotic influenza virus subtypes continue to be a great public health concern. We report detailed structural analysis and glycan microarray data for recombinant hemagglutinins from A(H6N1) and A(H10N8) viruses, isolated from human infections in 2013, and compare them with hemagglutinins of avian origin. This is the first structural report of an H6 hemagglutinin, and our results should further the understanding of these viruses and provide useful information to aid in the continuous surveillance of these zoonotic influenza viruses. Based on the serological reactivity of influenza A virus surface glycoproteins, hemagglutinin (HA) and neuraminidase (NA), 18 HA (H1 to H18) and 11 NA (N1 to N11) variants have been identified in aquatic birds and bats (1-3). The Centers for Disease Control and Prevention estimates that annually, seasonal influenza viruses can infect up to 20% of the human population (http: //www.cdc.gov/flu). In the United States alone, estimates of influenza-associated deaths range from 3,000 to 49,000 per annum (4). In the last 100 years, three influenza virus subtypes have successfully adapted to the human population, causing four pandemics: H1N1 in 1918 and 2009, H2N2 in 1957, and H3N2 in 1968 (5-8). Avian influenza viruses have become endemic in domestic poultry in certain parts of the world, and a growing number of human cases of avian influenza infection with different subtypes [A(H5N1), A(H5N6), A(H7N2), A(H7N3), A(H7N7), and A(H9N2)] have been reported in recent years (9-14), causing significant public health concern.During 2013, three novel avian influenza A viruses, A(H7N9), A(H10N8), and A(H6N1), were isolated from human patients. The first human infection with an avian influenza A(H7N9) virus was reported in March 2013 near Shanghai, China, and its emergence led to a human epidemic in that country (15). Indeed, as of October 2014, the virus had spread widely to a number of provinces and municipalities in China and resulted in over 450 human cases, with an ϳ38% mortality rate. Not surprisingly, most research has focused on this A(H7N9) virus due to its rapid spread and severity. Whole-genome genetic analysis highlighted the fact that the six internal genes of the virus all originated from H9N2 viruses and may contribute to the increased pathogenicity observed in human infections (16).In mid-201...

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confidence: 81%

Structure and Receptor Binding Preferences of Recombinant Hemagglutinins from Avian and Human H6 and H10 Influenza A Virus Subtypes

Yang

Carney

Chang

et al. 2015

J Virol

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“…In 2013, H10N8 virus caused three human infections in China, two of which were fatal (21,22). Although sequence information about the H10 viruses is increasing (23)(24)(25)(26), the biologic properties of these viruses remain largely unknown.…”

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confidence: 99%

Genetics, Receptor Binding, and Virulence in Mice of H10N8 Influenza Viruses Isolated from Ducks and Chickens in Live Poultry Markets in China

Deng

Shi

Wang

et al. 2015

J Virol

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bWe analyzed eight H10N8 viruses isolated from ducks and chickens in live poultry markets from 2009 to 2013 in China. These viruses showed distinct genetic diversity and formed five genotypes: the four duck isolates formed four different genotypes, whereas the four chicken viruses belong to a single genotype. The viruses bound to both human-and avian-type receptors, and four of the viruses caused 12.7% to 22.5% body weight loss in mice. Influenza viruses bearing the H10 subtype hemagglutinin (HA) have been detected in avian species across wide geographic areas. The first H10 isolate, an H10N7 virus, was detected in chickens in Germany in 1949 (1, 2). Since then, viruses bearing H10 HA and different neuraminidase (NA) subtypes have been widely detected in wild birds and domestic poultry around the world (3-19). Moreover, an H10N4 virus caused an outbreak of a respiratory disease in mink in Sweden in 1984 (20), and more recently, several U.S. turkey workers tested seropositive for H10 influenza virus (15). In March 2010, an H10N7 virus caused an outbreak on a chicken farm in Australia; after processing clinically normal birds from the farm, seven abattoir workers reported conjunctivitis and minor upper respiratory tract symptoms and H10 virus infection was detected in two of the seven workers (11). In 2013, H10N8 virus caused three human infections in China, two of which were fatal (21, 22). Although sequence information about the H10 viruses is increasing (23-26), the biologic properties of these viruses remain largely unknown.A total of eight H10N8 influenza viruses were isolated from ducks and chickens during our routine surveillance from To investigate the genetic relationships among these viruses, we sequenced the genomes of all eight viruses. The amino acid motif at the HA cleavage site of these isolates is -R-, which is a characteristic of viruses of low pathogenicity in chickens. The eight genes of the viruses showed distinct diversity, with the HA, NA, PB2, PB1, PA, NP, M, and NS genes of the eight viruses sharing 94.7 to 100, 78.4 to 99.9, 89.9 to 100, 90.1 to 100, 89.7 to 100, 90.0 to 99.9, 90.5 to 100, and 89.3 to 99.9% identity, respectively, at the nucleotide level. The HA, PA, and NS genes each formed two branches in their phylogenetic trees (Fig. 1A, E, and H), whereas the NA, PB2, PB1, and M genes formed three branches each in their phylogenetic trees (Fig. 1B, C, D, and G) and the NP gene formed four branches in its phylogenetic tree (Fig. 1F). Of note, the six internal genes of the H10N8 viruses in branch 1 were clustered with the corresponding genes of H9N2 influenza viruses (Fig. 1C to H), and the NA gene in group 1 belongs to the North American lineage (Fig. 1B).On the basis of this genomic diversity, we divided the viruses into five genotypes (Table 1). Of note, the four duck viruses belong to four different genotypes, whereas the four chicken viruses belong to a single genotype, and the eight gene segments of the CK/JX/S3581/13 virus shared 99.2% to 99.9% identity with the human H10N8 virus...

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“…Subsequent studies showed the presence of H10N8 in poultry and live bird markets in this province [3, 5, 6]. Serological evidence for the virus was also found in poultry workers in the neighboring Guangdong province and in feral dogs that frequent live bird markets in the same province [3, 7].…”

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confidence: 96%

An H10N8 influenza virus vaccine strain and mouse challenge model based on the human isolate A/Jiangxi-Donghu/346/13

Wohlbold

Hirsh

Krammer

2015

Vaccine

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Three human cases of H10N8 viruses were reported in China in late 2013 and early 2014, two of which were fatal. This was the first time the H10N8 subtype has been detected in humans and no vaccine candidates or antibody therapy has been developed for these viruses so far. We developed an H10N8 vaccine candidate virus based on A/Jiangxi-Donghu/346/13 that can also be used in a murine challenge model for vaccine and monoclonal antibody research. The vaccine virus is a 6:2 re-assortant virus expressing the surface glycoproteins of A/Jiangxi-Donghu/346/13 on an A/Puerto Rico/8/34 backbone. Vaccination with inactivated challenge virus or recombinant hemagglutinin or neuraminidase derived from this strain protected mice from viral challenge.

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Investigation of avian influenza virus in poultry and wild birds due to novel avian-origin influenza A(H10N8) in Nanchang City, China

Cited by 19 publications

References 29 publications

Structure and Receptor Binding Preferences of Recombinant Hemagglutinins from Avian and Human H6 and H10 Influenza A Virus Subtypes

Structure and Receptor Binding Preferences of Recombinant Hemagglutinins from Avian and Human H6 and H10 Influenza A Virus Subtypes

Genetics, Receptor Binding, and Virulence in Mice of H10N8 Influenza Viruses Isolated from Ducks and Chickens in Live Poultry Markets in China

An H10N8 influenza virus vaccine strain and mouse challenge model based on the human isolate A/Jiangxi-Donghu/346/13

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