Investigation of aquaporins and apparent diffusion coefficient from ultra-high b-values in a rat model of diabetic nephropathy

Wang, Yu; Zhang, Heng; Zhang, Ru‐Zhi; Zhao, Zhonghua; Xu, Ziqian; Wang, Lei; Liu, Rongbo

doi:10.1186/s41747-017-0016-3

Cited by 12 publications

(7 citation statements)

References 24 publications

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“…When the b -value is greater than 2,000 s/mm 2 , it is most likely to reflect the effect of Aquaporin-4 on water transport ( Zeng et al, 2018 ). According to previous studies ( Xueying et al, 2015 ; Hu et al, 2017 ; Wang et al, 2017 ; Xue et al, 2019 ), ADC mh may be associated with water transportation by aquaporins. We, therefore, speculated whether the large value on ADC mh represents the high expression of AQP4 which plays a crucial role in cerebral ischemia.…”

Section: Discussionmentioning

confidence: 81%

“…Multi-high b -value ADCs from multi-high- b values has been proved to be a useful parameter for evaluating brain damage in Parkinson’s disease ( Xueying et al, 2015 ), glioma grading ( Hu et al, 2017 ; Tan et al, 2018 ), early detection of kidney damage in diabetic nephropathy ( Wang et al, 2017 ), and monitoring the pathophysiological changes of AD ( Xue et al, 2019 ). In our study, multi-high b -value ADC was applied to a focal cerebral ischemia rat model.…”

Section: Discussionmentioning

confidence: 99%

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Heterogeneity evaluation of multi-high b-value apparent diffusion coefficient on cerebral ischemia in MCAO rat

et al. 2022

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PurposeTo assess brain damage in a rat model of cerebral ischemia based on apparent diffusion coefficient (ADC) data obtained from multi-high b-values and evaluate the relationship between Aquaporin 4 (AQP4) expression and ADC.MethodsThirty eight male Sprague–Dawley rats were randomized into two groups: (1) sham controls (n = 6) and (2) cerebral ischemia (successful model, n = 19). All rats underwent diffusion-weighted imaging (DWI) with both standard b-values and multi-high b-values (2,500–4,500 s/mm2) using a 3.0-T device. Standard ADC (ADCst) maps and multi-high b-value ADCs (ADCmh) were calculated, respectively. Aquaporin 4 expression was quantified using Western blot. Relative values of ADCst and ADCmh, AQP4 expression were compared between the sham group and the ischemia group. Correlations between ADC values and AQP4 expression were evaluated.ResultsAt 0.5 h after suture insertion, the value of ADCmh on the lesion was obviously decreased, and there was no difference in lesion volume when compared with ADCst. After reperfusion, besides similar regions where ADCst values decreased, we also found additional large values on ADCmh within the cortex of the ipsilateral side or surrounding the lesion. The lesion evolution of the large value on ADCmh was quite different from other indicators. But the total ADCmh values were still significantly associated with ADCst. The AQP4 protein expression level was appreciably increased after middle cerebral artery occlusion (MCAO), but there was no correlation between AQP4 expression either with ADCmh or ADCst.ConclusionWe found the large values on ADCmh during the progression of cerebral infarction is varied, but there was no correlation between ADCmh values and AQP4 expression. ADCmh may indicate the heterogeneity of ischemia lesions, but the underlying pathological basis should be further explored.

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Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Heterogeneity evaluation of multi-high b-value apparent diffusion coefficient on cerebral ischemia in MCAO rat

et al. 2022

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“…With the knowledge of slower water transportation speed ( 36 ) resulted from the AQPs, previous studies indicated that ADC derived in the high- b value range might be one potential marker for AQPs expressions in gliomas ( 5 ) or other diseases ( 25 , 37 ). However, our results showed that the ADC uh was not completely independent from other DWI metrics such as ADC st to independently indicate AQPs expression.…”

Section: Discussionmentioning

confidence: 99%

Study of Diffusion Weighted Imaging Derived Diffusion Parameters as Biomarkers for the Microenvironment in Gliomas

et al. 2021

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ObjectivesTo explore the efficacy of diffusion weighted imaging (DWI)-derived metrics under different models as surrogate indicators for molecular biomarkers and tumor microenvironment in gliomas.MethodsA retrospective study was performed for 41 patients with gliomas. The standard apparent diffusion coefficient (ADCst) and ADC under ultra-high b values (ADCuh) (b values: 2500 to 5000 s/mm2) were calculated based on monoexponential model. The fraction of fast diffusion (f), pseudo ADC (ADCfast) and true ADC (ADCslow) were calculated by bi-exponential model (b values: 0 to 2000 s/mm2). The apparent diffusional kurtosis (Kapp) was derived from the simplified diffusion kurtosis imaging (DKI) model (b values: 200 to 3000 s/mm2). Potential correlations between DWI parameters and immunohistological indices (i.e. Aquaporin (AQP)1, AQP4, AQP9 and Ki-67) were investigated and DWI parameters were compared between high- and low-grade gliomas, and between tumor center and peritumor. Receiver operator characteristic (ROC) curve and area under the curve (AUC) were calculated to determine the performance of independent or combined DWI parameters in grading gliomas.ResultsThe ADCslow and ADCuh at tumor center showed a stronger correlation with Ki-67 than other DWI metrics. The ADCst, ADCslow and ADCuh at tumor center presented correlations with AQP1 and AQP4 while AQP9 did not correlate with any DWI metric. Kapp showed a correlation with Ki-67 while no significant correlation with AQPs. ADCst (p < 0.001) and ADCslow (p = 0.001) were significantly lower while the ADCuh (p = 0.006) and Kapp (p = 0.005) were significantly higher in the high-grade than in the low-grade gliomas. ADCst, f, ADCfast, ADCslow, ADCuh, Kapp at the tumor center had significant differences with those in peritumor when the gliomas grade became high (p < 0.05). Involving ADCuh and Kapp simultaneously into an independent ADCst model (AUC = 0.833) could further improve the grading performance (ADCst+ADCuh+Kapp: AUC = 0.923).ConclusionDifferent DWI metrics fitted within different b-value ranges (low to ultra-high b values) have different efficacies as a surrogate indicator for molecular expression or microstructural complexity in gliomas. Further studies are needed to better explain the biological meanings of these DWI parameters in gliomas.

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“…When room temperature was reached, incubation of slides with 1% bovine serum albumin (BSA) for 1 h was done and then incubation with primary antibodies overnight at 4 °C was performed. Three primary antibodies were used in this study: 1-rabbit polyclonal primary antibodies raised against aquaporin-1 (AQP1) (GB11311-1, Servicebio, China at 1:1000 dilution); 2-rabbit polyclonal primary antibodies raised against aquaporin-4 (AQP4) (GB11529, Servicebio, China at 1:1200 dilution) for assessment of their role in diabetes and intermittent fasting [89]; and 3-rabbit polyclonal primary antibodies raised against microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3-II) (YPA1652, Chongqing Biospes, China at 1:200 dilution) for assessment of autophagy [90]. The sections were then rinsed with PBS followed by these three steps: Step 1, incubation with horseradish peroxidase (HRP) conjugated secondary antibodies for 0.5 h at 37 °C; Step 2, incubation with labelled streptavidin-biotin for 0.5 h at 37 °C; and…”

Section: Immunohistochemical Study and Morphometric Analysismentioning

confidence: 99%

Streptozotocin- induced changes of aquaporin 1 and 4, oxidative stress and autophagy in submandibular and parotid salivary glands and the possible ameliorative effect of intermittent fasting on these changes: immunohistochemical and molecular study in rats.

Taha

Alghamdi

Hanan

et al. 2022

Preprint

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Salivary glands are greatly responsible for maintaining oral tissue homeostasis by the secretion of saliva. This study was designed to investigate the changes of aquaporin 1 and 4, oxidative stress and autophagy in submandibular and parotid salivary glands of diabetic rats and the possible ameliorative effect of intermittent fasting on these changes. Fifty adult male rats were divided into control and experimental groups. Experimental diabetes was induced by a single intraperitoneal injection of streptozotocin. After diabetic induction, the experimental group was divided into two groups (diabetic without intermittent fasting and diabetic with intermittent fasting). Animals were sacrificed two and four weeks after induction of diabetes. Intermittent fasting significantly decreased malondialdehyde and significantly elevated reduced glutathione (GSH) in the submandibular and parotid glands compared to those in diabetic rats. The salivary secretions were also significantly spared histologically in diabetics with intermittent fasting groups. As well, intermittent fasting up-regulated aquaporin 1 in both glands, while aquaporin 4 was only elevated in submandibular gland. Immunolocalization and gene expression of Lc3-II was higher in diabetic salivary glands than in fasting glands. In conclusion, these findings highlight the pathological role of autophagy in diabetic sub-mandibular and parotid glands and provide potential target for therapeutic role of intermittent fasting to ameliorate submandibular and parotid glands dysfunction in type I diabetes mellitus.

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Investigation of aquaporins and apparent diffusion coefficient from ultra-high b-values in a rat model of diabetic nephropathy

Cited by 12 publications

References 24 publications

Heterogeneity evaluation of multi-high b-value apparent diffusion coefficient on cerebral ischemia in MCAO rat

Heterogeneity evaluation of multi-high b-value apparent diffusion coefficient on cerebral ischemia in MCAO rat

Study of Diffusion Weighted Imaging Derived Diffusion Parameters as Biomarkers for the Microenvironment in Gliomas

Streptozotocin- induced changes of aquaporin 1 and 4, oxidative stress and autophagy in submandibular and parotid salivary glands and the possible ameliorative effect of intermittent fasting on these changes: immunohistochemical and molecular study in rats.

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