Investigating the cytotoxicity of iron oxide nanoparticles in in vivo and in vitro studies

Ghasempour, Sarieh; Shokrgozar, Mohammad Ali; Ghasempour, Roghayeh; Alipour, Mohsen

doi:10.1016/j.etp.2015.07.005

Cited by 25 publications

(17 citation statements)

References 28 publications

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“…Data was analyzed using one‐way or two‐way analysis of variances (ANOVA) followed by posthoc Tukey comparison test using the mean of each treatment group in Prism (Version 8). Where relevant, data are presented as the mean ± standard error (Ghasempour, Shokrgozar, Ghasempour, & Alipour, ). Mean of every treatment was compared to the control and significance was determine and denoted by (*) for p < .05, (**) for p < .01, (***) for p < .001, and for p < .0001.…”

Section: Methodsmentioning

confidence: 99%

Biodistribution and toxicity of epitope‐functionalized dextran iron oxide nanoparticles in a pregnant murine model

Bolandparvaz

Vapniarsky

Harriman

et al. 2020

J Biomedical Materials Res

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In pursuit of a preventive therapeutic for maternal autoantibody‐related (MAR) autism, we assessed the toxicity, biodistribution, and clearance of a MAR specific peptide‐functionalized dextran iron oxide nanoparticle system in pregnant murine dams. We previously synthesized ~15 nm citrate‐coated dextran iron oxide nanoparticles (DIONPs), surface‐modified with polyethylene glycol and MAR peptides to produce systems for nanoparticle‐based autoantibody reception and entrapments (SNAREs). First, we investigated their immunogenicity and MAR lactate dehydrogenase B antibody uptake in murine serum in vitro. To assess biodistribution and toxicity, as well as systemic effects, we performed in vivo clinical and post mortem pathological evaluations. We observed minimal production of inflammatory cytokines—interleukin 10 (IL‐10) and IL‐12 following in vitro exposure of macrophages to SNAREs. We established the maximum tolerated dose of SNAREs to be 150 mg/kg at which deposition of iron was evident in the liver and lungs by histology and magnetic resonance imaging but no concurrent evidence of liver toxicity or lung infarction was detected. Further, SNAREs exhibited slower clearance from the maternal blood in pregnant dams compared to DIONPs based on serum total iron concentration. These findings demonstrated that the SNAREs have a prolonged presence in the blood and are safe for use in pregnant mice as evidenced by no associated organ damage, failure, inflammation, and fetal mortality. Determination of the MTD dose sets the basis for future studies investigating the efficacy of our nanoparticle formulation in a MAR autism mouse model.

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Section: Methodsmentioning

confidence: 99%

Biodistribution and toxicity of epitope‐functionalized dextran iron oxide nanoparticles in a pregnant murine model

Bolandparvaz

Vapniarsky

Harriman

et al. 2020

J Biomedical Materials Res

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“…Positively charged IONPs were shown to be more toxic, since they undergo nonspecific interactions and adsorptive endocytosis with the negatively charged cell membrane, thus increasing their intracellular accumulation and affecting cell membrane integrity [ 218 ]. Other factors such as concentration, type of coating, form of administration, as well as the cell line may explain the different results for IONPs toxicity [ 21 , 22 , 23 , 25 , 26 , 66 , 71 , 72 , 96 , 97 , 98 , 163 , 214 , 215 , 219 , 220 , 221 , 222 , 223 , 224 , 225 , 226 , 227 , 228 , 229 , 230 , 231 , 232 , 233 , 234 , 235 , 236 , 237 , 238 , 239 , 240 , 241 , 242 , 243 , 244 , 245 , 246 , 247 , 248 ], as shown in Table 1 .…”

Section: Ionps Toxicitymentioning

confidence: 99%

Iron Oxide Nanoparticles for Biomedical Applications: A Perspective on Synthesis, Drugs, Antimicrobial Activity, and Toxicity

et al. 2018

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Medical applications and biotechnological advances, including magnetic resonance imaging, cell separation and detection, tissue repair, magnetic hyperthermia and drug delivery, have strongly benefited from employing iron oxide nanoparticles (IONPs) due to their remarkable properties, such as superparamagnetism, size and possibility of receiving a biocompatible coating. Ongoing research efforts focus on reducing drug concentration, toxicity, and other side effects, while increasing efficacy of IONPs-based treatments. This review highlights the methods of synthesis and presents the most recent reports in the literature regarding advances in drug delivery using IONPs-based systems, as well as their antimicrobial activity against different microorganisms. Furthermore, the toxicity of IONPs alone and constituting nanosystems is also addressed.

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“…5D, incubation with nanorods did not cause significant early apoptotic and late apoptotic/necrotic cell death after 24 or 48 h of incubation, evaluated by apoptotic Annexin V/PI staining assay. In summary, this iron oxide nanorods have a high degree of biocompatibility related to other ellipsoidal magnetic nanoparticles, bearing in mind that cytotoxicity has been evaluated for periods not exceeding 24 h46.…”

mentioning

confidence: 99%

In-situ particles reorientation during magnetic hyperthermia application: Shape matters twice

Simeonidis

Morales

Marciello

et al. 2016

Sci Rep

103

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Promising advances in nanomedicine such as magnetic hyperthermia rely on a precise control of the nanoparticle performance in the cellular environment. This constitutes a huge research challenge due to difficulties for achieving a remote control within the human body. Here we report on the significant double role of the shape of ellipsoidal magnetic nanoparticles (nanorods) subjected to an external AC magnetic field: first, the heat release is increased due to the additional shape anisotropy; second, the rods dynamically reorientate in the orthogonal direction to the AC field direction. Importantly, the heating performance and the directional orientation occur in synergy and can be easily controlled by changing the AC field treatment duration, thus opening the pathway to combined hyperthermic/mechanical nanoactuators for biomedicine. Preliminary studies demonstrate the high accumulation of nanorods into HeLa cells whereas viability analysis supports their low toxicity and the absence of apoptotic or necrotic cell death after 24 or 48 h of incubation.

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Investigating the cytotoxicity of iron oxide nanoparticles in in vivo and in vitro studies

Cited by 25 publications

References 28 publications

Biodistribution and toxicity of epitope‐functionalized dextran iron oxide nanoparticles in a pregnant murine model

Biodistribution and toxicity of epitope‐functionalized dextran iron oxide nanoparticles in a pregnant murine model

Iron Oxide Nanoparticles for Biomedical Applications: A Perspective on Synthesis, Drugs, Antimicrobial Activity, and Toxicity

In-situ particles reorientation during magnetic hyperthermia application: Shape matters twice

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