Inversion of the G DNA segment of phage Mu controls phage infectivity

Kamp, Dietmar; Kahmann, Regine; Zipser, David; Broker, Thomas R.; Chow, Louise T.

doi:10.1038/271577a0

Cited by 137 publications

(58 citation statements)

References 16 publications

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“…Genetic engineering might thus offer the possibility to extend the host range of a single master T4-like phage. In fact, some coliphages use this strategy naturally: phage Mu inverts the orientation of the receptorinteracting gene by a phage-encoded recombinase (Kamp et al, 1978), and Scholl et al (2001) described a coliphage possessing two different tail fibre proteins that showed the combined host range of phages containing one or the other protein. About 30 different O serotypes must be lysed to cover the majority of EPEC and ETEC strains worldwide (Robins-Browne, 1987;Goodridge et al, 2003).…”

Section: Why E Coli?mentioning

confidence: 99%

Phage therapy: the Escherichia coli experience

2005

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Phages have been proposed as natural antimicrobial agents to fight bacterial infections in humans, in animals or in crops of agricultural importance. Phages have also been discussed as hygiene measures in food production facilities and hospitals. These proposals have a long history, but are currently going through a kind of renaissance as documented by a spate of recent reviews. This review discusses the potential of phage therapy with a specific example, namely Escherichia coli.

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Section: Why E Coli?mentioning

confidence: 99%

Phage therapy: the Escherichia coli experience

2005

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“…In addition, these sequence analyses showed that both units are transcribed in the rightward direction. Gene gin is expressed both in the lysogenic state and during lytic development (Kamp et al, 1978;Symonds & Coelho, 1978;Bukhari & Ambrosio, 1978) but expression of morn has been demonstrated only during lytic development (Toussaint, 1976). Hattman & Ives (1984) detected transcripts for the gin and morn regions from the 20th min and demonstrated a rapid increase of their synthesis after the 25th min of the lytic cycle.…”

confidence: 99%

Transcriptional Mapping of the Bacteriophage Mu DNA

Barron

Bade

1988

Journal of General Virology

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SUMMARYThe transcription of temperate phage Mu throughout lytic development was analysed quantitatively by hybridization of pulse-labelled RNA to full-length Mu DNA and to plasmids that define Mu DNA segments covering the whole phage genome. The transcription rate (i.e. binding data corrected for the incorporation rate of the radioactive precursor, for the size of the DNA template, and for the number of phage genomes present in the bacterium at the time of analysis) revealed three defined phases of Mu transcription: early (0 to 9 min), intermediate (between 9 and the interval 14 to 17 min) and late (from the interval 14 to 17 min onward). The analysis also revealed that the region comprising the genes involved in phage morphogenesis was organized into two independent 'late' transcription units.

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“…(ii) amplifications (3)(4)(5)(6)(7)(8)(9) ; for example the mouse dihydrofolate reductase gene (3). (iii) transpositions (10)(11)(12)(13)(14) ; for example the genes which confer different mating types on yeast,[see review of Leupold (10) for references] and (iv) inversions (15)(16)(17); at present described only for prokaryotes, for example the flagellin gene of salmonella (15).…”

Section: Introductionmentioning

confidence: 99%

A detailed comparison of the 5′-end of the ovalbumin gene cloned from chicken oviduct and erythrocyte DNA

1980

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We have examined homologous fragments of DNA cloned from two different tissues for changes in the DNA sequence which might be related to tissue specific gene expression. The 5' end of the chicken ovalbumin gene was cloned from oviduct or erythrocyte DNA using cosmids as vectors. We have compared the two clones obtained by restriction enzyme digestions, analysis of heteroduplexes by electron microscopy or S1 nuclease digestion and by DNA sequencing. Our results show that whereas no alteration occured in the region of the gene assumed to be of importance for the control of transcription, a 4 nucleotide deletion/insertion was detected in the first intron of the ovalbumin gene.

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Inversion of the G DNA segment of phage Mu controls phage infectivity

Cited by 137 publications

References 16 publications

Phage therapy: the Escherichia coli experience

Phage therapy: the Escherichia coli experience

Transcriptional Mapping of the Bacteriophage Mu DNA

A detailed comparison of the 5′-end of the ovalbumin gene cloned from chicken oviduct and erythrocyte DNA

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