This introductory article situates Ministries of Foreign Affairs (MFAs) as gendered institutions at the intersection between domestic and international relations. Based on an extensive literature review and analysis of articles on Australian, Bulgarian, Czech, Japanese, Turkish, UK, and US MFAs in this special issue, we claim that research on gender and MFAs has made important contributions to diplomatic studies by deepening, challenging, and diversifying understandings of what MFAs are; MFAs’ institutional structures; and power struggles within MFAs. MFA relations with other actors remain decidedly understudied from a gender perspective, however. Future research on gender and MFAs should direct attention to these relationships, including how they shape MFAs as gendered institutions. Future studies would also benefit from global and intersectional analyses of multiple axes of power and differentiation. By identifying research questions, new theoretical perspectives, and largely unapplied research designs, we hope to facilitate the pursuit of such studies.