Intubation Studies of the Human Small Intestine. Xxiii. A Method of Determining Digestive Activity in Any Portion of the Gastro-Intestinal Tract, With Some Measurements of Protein Digestion in the Stomach and Small Intestine 12

Elsom, Kendall A.; Chornock, Francis W.; Dickey, Francis G.

doi:10.1172/jci101357

Cited by 6 publications

(1 citation statement)

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“…However, it was shown for the first time that the granulocytes inflammatory cells, especially eosinophils, were elevated in the gut of infected wild-type mice. This finding corroborates rare reports in medical literature that present eosinophilia as a clinical manifestation in patients with brucellosis [ 43 , 44 ]. Albeit high levels of eosinophils were found on the Nlrp6 -/- mice, both in the gut and blood, this increase may be associated with a greater intestinal defense against the invasion of B .…”

Section: Discussionsupporting

confidence: 92%

NLRP6-associated host microbiota composition impacts in the intestinal barrier to systemic dissemination of Brucella abortus

et al. 2021

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Brucella abortus is a Gram-negative bacterium responsible for a worldwide zoonotic infection—Brucellosis, which has been associated with high morbidity rate in humans and severe economic losses in infected livestock. The natural route of infection is through oral and nasal mucosa but the invasion process through host gut mucosa is yet to be understood. Studies have examined the role of NLRP6 (NOD-like receptor family pyrin domain-containing-6 protein) in gut homeostasis and defense against pathogens. Here, we investigated the impact of gut microbiota and NLRP6 in a murine model of Ba oral infection. Nlrp6-/- and wild-type (WT) mice were infected by oral gavage with Ba and tissues samples were collected at different time points. Our results suggest that Ba oral infection leads to significant alterations in gut microbiota. Moreover, Nlrp6-/- mice were more resistant to infection, with decreased CFU in the liver and reduction in gut permeability when compared to the control group. Fecal microbiota transplantation from WT and Nlrp6-/- into germ-free mice reflected the gut permeability phenotype from the donors. Additionally, depletion of gut microbiota by broad-spectrum-antibiotic treatment prevented Ba replication in WT while favoring bacterial growth in Nlrp6-/-. Finally, we observed higher eosinophils in the gut and leukocytes in the blood of infected Nlrp6-/- compared to WT-infected mice, which might be associated to the Nlrp6-/- resistance phenotype. Altogether, these results indicated that gut microbiota composition is the major factor involved in the initial stages of pathogen host replication and partially also by the resistance phenotype observed in Nlrp6 -/- mice regulating host inflammation against Ba infection.

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Section: Discussionsupporting

confidence: 92%