Introduction of the South American slipper limpet Crepipatella dilatata in Europe: Secondary spread in the Northeast Atlantic and first records for the Mediterranean Sea

Abstract

The slipper limpet Crepipatella dilatata, native to Chile and Argentina, was introduced in north-western Spain in 2005 and since then has spread to the Galician and Cantabrian coasts. In this work, we provide the first molecularly validated record of an established population of C. dilatata in central Portugal (Aveiro), and of reproductive individuals (i.e. brooding females) of C. dilatata in the Mediterranean Sea, from three Italian mussel farms (in eastern Sardinia and northern Adriatic). DNA barcoding and phylogenetic analyses based on mitochondrial markers indicate that limpets from Italian farms belong to the C. dilatata ‘clade1’, whereas limpets collected in Aveiro cluster within the C. dilatata ‘clade2’. All these limpets share the same COI haplotypes with introduced populations from Galicia. The arrival of C. dilatata to Europe is undoubtedly due to transoceanic live mussel trade from southern Chile to Galicia. Mussel trade and farming are also the most likely drivers for the post-border dispersal of C. dilatata from Spain to Portugal and to Italy. Several countries have a potential role as a hub for the primary and secondary dispersal of slipper limpets towards and within Europe. This calls the attention for future research focused on assessing the current occurrence and ecological impact of established populations of slipper limpets within and nearby mussel farms along the eastern Atlantic and Mediterranean coasts.