Intrinsically disordered protein domain of human ameloblastin in synthetic fusion with calmodulin increases calmodulin stability and modulates its function

Zouharová, Monika; Vymětal, Jiří; Bednářová, Lucie; Vaněk, Ondřej; Heřman, Petr; Vetyskova, Veronika; Postulkova, Klara; Lingstaadas, Petter S.; Vondrášek, Jiřı́; Boušová, Kristýna

doi:10.1016/j.ijbiomac.2020.11.216

“…Among the signaling pathways, wingless (Wnt), DNA mismatch repair homeobox 1/2 (Msx1/2), bone morphogenetic protein (Bmp), and runt-related transcription factors 2 (Runx2), and disheveled homologous 2 (Dlx2) are involved in the mineralization process of enamel and dentin. [2,[34][35][36][37] These signaling proteins regulate the expression and secretion of ameloblastin (AMBN), amelogenin (AMELX), enamelin (ENAM), alkaline phosphatase, matrix metalloproteinase 20 (MMP20) (ALP), and kallikrein-related peptidase 4 (KLK4). [38][39][40][41][42] This process occurs after the tenth week of embryonic in humans and after the 13th day of embryonic in mice.…”

Section: Introductionmentioning

confidence: 99%

Physicochemical understanding of biomineralization by molecular vibrational spectroscopy: From mechanism to nature

Liu

¹

,

Jiang

²

,

Liu

³

et al. 2023

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The process and mechanism of biomineralization and relevant physicochemical properties of mineral crystals are remarkably sophisticated multidisciplinary fields that include biology, chemistry, physics, and materials science. The components of the organic matter, structural construction of minerals, and related mechanical interaction, etc., could help to reveal the unique nature of the special mineralization process. Herein, the paper provides an overview of the biomineralization process from the perspective of molecular vibrational spectroscopy, including the physicochemical properties of biomineralized tissues, from physiological to applied mineralization. These physicochemical characteristics closely to the hierarchical mineralization process include biological crystal defects, chemical bonding, atomic doping, structural changes, and content changes in organic matter, along with the interface between biocrystals and organic matter as well as the specific mechanical effects for hardness and toughness. Based on those observations, the special physiological properties of mineralization for enamel and bone, as well as the possible mechanism of pathological mineralization and calcification such as atherosclerosis, tumor micro mineralization, and urolithiasis are also reviewed and discussed. Indeed, the clearly defined physicochemical properties of mineral crystals could pave the way for studies on the mechanisms and applications.

show abstract

“…Among the signaling pathways, wingless (Wnt), DNA mismatch repair homeobox 1/2 (Msx1/2), bone morphogenetic protein (Bmp), and runt-related transcription factors 2 (Runx2), and disheveled homologous 2 (Dlx2) are involved in the mineralization process of enamel and dentin. [2,[34][35][36][37] These signaling proteins regulate the expression and secretion of ameloblastin (AMBN), amelogenin (AMELX), enamelin (ENAM), alkaline phosphatase, matrix metalloproteinase 20 (MMP20) (ALP) and kallikrein-related peptidase 4 (KLK4). [38][39][40][41][42] This process occurs after the tenth week of embryonic in humans and after the 13th day of embryonic in mice.…”

Section: Introductionmentioning

confidence: 99%

Physicochemical Understanding of Biomineralization by Molecular Vibrational Spectroscopy: From Mechanism to Nature

Liu¹,

Jiang²,

Liu³

et al. 2023

Preprint

0

View full text Add to dashboard Cite

The process and mechanism of biomineralization and relevant physicochemical properties of mineral crystals are remarkably sophisticated multidisciplinary fields that include biology, chemistry, physics, and materials science. The components of the organic matter, structural construction of minerals, and related mechanical interaction, etc., could help to reveal the unique nature of the special mineralization process. Herein, the paper provides an overview of the biomineralization process from the perspective of molecular vibrational spectroscopy, including the physicochemical properties of biomineralized tissues, from physiological to applied mineralization. These physicochemical characteristics closely to the hierarchical mineralization process include biological crystal defects, chemical bonding, atomic doping, structural changes, and content changes in organic matter, along with the interface between biocrystals and organic matter as well as the specific mechanical effects for hardness and toughness. Based on those observations, the special physiological properties of mineralization for enamel and bone, as well as the possible mechanism of pathological mineralization and calcification such as atherosclerosis, tumor micro mineralization, and urolithiasis are also reviewed and discussed. Indeed, the clearly defined physicochemical properties of mineral crystals could pave the way for studies on the mechanisms and applications.

show abstract

Proteolytic profiles of two isoforms of human AMBN expressed in E. coli by MMP-20 and KLK-4 proteases

Vetyskova,

Hubalek,

Sulc

et al. 2024

Heliyon

0

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Intrinsically disordered protein domain of human ameloblastin in synthetic fusion with calmodulin increases calmodulin stability and modulates its function

Cited by 4 publications

References 74 publications

Physicochemical understanding of biomineralization by molecular vibrational spectroscopy: From mechanism to nature

Physicochemical understanding of biomineralization by molecular vibrational spectroscopy: From mechanism to nature

Physicochemical Understanding of Biomineralization by Molecular Vibrational Spectroscopy: From Mechanism to Nature

Proteolytic profiles of two isoforms of human AMBN expressed in E. coli by MMP-20 and KLK-4 proteases

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