Intrinsic, Hox-Dependent Cues Determine the Fate of Skeletal Muscle Precursors

Alvares, Lúcia Elvira; Schubert, Frank; Thorpe, Colin; Mootoosamy, Roy C.; Cheng, Louise; Parkyn, Gary; Lumsden, Andrew; Dietrich, Susanne

doi:10.1016/s1534-5807(03)00263-6

Cited by 99 publications

(89 citation statements)

References 39 publications

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“…Interestingly, in the cardiac domain our data demonstrate that lb is able to positively regulate the expression of tin and the effector of RTK pathway pointed (pnt), both involved in cardiac cell fate specification (Alvares et al 2003;Zaffran et al 2006). These findings are consistent with earlier observations that the forced lb expression leads to the ectopic tin-positive cells within the dorsal vessel (Jagla et al 2002).…”

Section: The Lb-regulated Components Of the Cell Identity Codesupporting

confidence: 91%

Genome-wide view of cell fate specification: ladybird acts at multiple levels during diversification of muscle and heart precursors

Junion¹,

Bataillé²,

Jagla³

et al. 2007

Genes Dev.

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Correct diversification of cell types during development ensures the formation of functional organs. The evolutionarily conserved homeobox genes from ladybird/Lbx family were found to act as cell identity genes in a number of embryonic tissues. A prior genetic analysis showed that during Drosophila muscle and heart development ladybird is required for the specification of a subset of muscular and cardiac precursors. To learn how ladybird genes exert their cell identity functions we performed muscle and heart-targeted genome-wide transcriptional profiling and a chromatin immunoprecipitation (ChIP)-on-chip search for direct Ladybird targets. Our data reveal that ladybird not only contributes to the combinatorial code of transcription factors specifying the identity of muscle and cardiac precursors, but also regulates a large number of genes involved in setting cell shape, adhesion, and motility. Among direct ladybird targets, we identified bric-a-brac 2 gene as a new component of identity code and inflated encoding ␣PS2-integrin playing a pivotal role in cell-cell interactions. Unexpectedly, ladybird also contributes to the regulation of terminal differentiation genes encoding structural muscle proteins or contributing to muscle contractility. Thus, the identity gene-governed diversification of cell types is a multistep process involving the transcriptional control of genes determining both morphological and functional properties of cells.[Keywords: Cell fate; microarray; ChEST; Drosophila; ladybird; muscle; heart] Supplemental material is available at http://www.genesdev.org.

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Section: The Lb-regulated Components Of the Cell Identity Codesupporting

confidence: 91%

Genome-wide view of cell fate specification: ladybird acts at multiple levels during diversification of muscle and heart precursors

Junion¹,

Bataillé²,

Jagla³

et al. 2007

Genes Dev.

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“…Our data show that Hox activity is superimposed on mesoderm-specific and positional information at the progenitor stage to establish the pattern of somatic muscles and acts at several independent steps during the muscle-specification process. Very few studies have addressed the role of Hox genes in controlling the fate of skeletal muscle precursors in vertebrates (Alvares et al, 2003). Our data provide a new framework for studying the integration of Hox information into the generic process of myogenesis and the generation of muscle diversity.…”

Section: Introductionmentioning

confidence: 78%

Multi-step control of muscle diversity by Hox proteins in the Drosophila embryo

et al. 2010

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SUMMARYHox transcription factors control many aspects of animal morphogenetic diversity. The segmental pattern of Drosophila larval muscles shows stereotyped variations along the anteroposterior body axis. Each muscle is seeded by a founder cell and the properties specific to each muscle reflect the expression by each founder cell of a specific combination of 'identity' transcription factors. Founder cells originate from asymmetric division of progenitor cells specified at fixed positions. Using the dorsal DA3 muscle lineage as a paradigm, we show here that Hox proteins play a decisive role in establishing the pattern of Drosophila muscles by controlling the expression of identity transcription factors, such as Nautilus and Collier (Col), at the progenitor stage. Highresolution analysis, using newly designed intron-containing reporter genes to detect primary transcripts, shows that the progenitor stage is the key step at which segment-specific information carried by Hox proteins is superimposed on intrasegmental positional information. Differential control of col transcription by the Antennapedia and Ultrabithorax/Abdominal-A paralogs is mediated by separate cis-regulatory modules (CRMs). Hox proteins also control the segment-specific number of myoblasts allocated to the DA3 muscle. We conclude that Hox proteins both regulate and contribute to the combinatorial code of transcription factors that specify muscle identity and act at several steps during the muscle-specification process to generate muscle diversity.

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“…It is possible that MEIS, HOXA11 and HOXA13 play a role in patterning of myogenic cells during regeneration; however, their expression is regulated by signals that myogenic cells receive from connective tissue cells. For example, in chicken, HOXA10 predisposes, in an autonomous way, the myogenic progenitors in the somite to a limb fate (Alvares et al, 2003). In Drosophila, the identity of muscle cells in different larval segments is believed to be the result of integration of extrinsic cues with autonomous expression of HOX homologues (Greig and Akam, 1993;Roy and Vijayraghavan, 1997).…”

Section: Discussionmentioning

confidence: 99%

Connective tissue cells, but not muscle cells, are involved in establishing the proximo-distal outcome of limb regeneration in the axolotl

Nacu

Glausch²,

Le³

et al. 2013

Development

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SUMMARYDuring salamander limb regeneration, only the structures distal to the amputation plane are regenerated, a property known as the rule of distal transformation. Multiple cell types are involved in limb regeneration; therefore, determining which cell types participate in distal transformation is important for understanding how the proximo-distal outcome of regeneration is achieved. We show that connective tissue-derived blastema cells obey the rule of distal transformation. They also have nuclear MEIS, which can act as an upper arm identity regulator, only upon upper arm amputation. By contrast, myogenic cells do not obey the rule of distal transformation and display nuclear MEIS upon amputation at any proximo-distal level. These results indicate that connective tissue cells, but not myogenic cells, are involved in establishing the proximo-distal outcome of regeneration and are likely to guide muscle patterning. Moreover, we show that, similarly to limb development, muscle patterning in regeneration is influenced by bcatenin signalling.

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Intrinsic, Hox-Dependent Cues Determine the Fate of Skeletal Muscle Precursors

Cited by 99 publications

References 39 publications

Genome-wide view of cell fate specification: ladybird acts at multiple levels during diversification of muscle and heart precursors

Genome-wide view of cell fate specification: ladybird acts at multiple levels during diversification of muscle and heart precursors

Multi-step control of muscle diversity by Hox proteins in the Drosophila embryo

Connective tissue cells, but not muscle cells, are involved in establishing the proximo-distal outcome of limb regeneration in the axolotl

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