Intrauterine growth restriction and placental gene expression in severe preeclampsia, comparing early-onset and late-onset forms

Nevalainen, Jaana; Skarp, Sini; Savolainen, Eeva‐Riitta; Ryynänen, Markku; Järvenpää, Jouko

doi:10.1515/jpm-2016-0406

Cited by 37 publications

(39 citation statements)

References 42 publications

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“…In silico miR screening analysis identified miR-1301 to have a predicted binding site in this region ( Figure 5A). miR-1301 is known to be dysregulated in early-onset preeclampsia [28], similar to HLA-G [29]. This finding strengthened our assumption that HLA-G might be regulated by miR-1301.…”

Section: Mir-1301 Is Not Involved In Hla-g Regulationsupporting

confidence: 79%

A Unique Regulation Region in the 3′ UTR of HLA-G with a Promising Potential

Reches

Berhani

Mandelboim

2020

IJMS

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Human leukocyte antigen G (HLA-G) is a non-classical human leukocyte antigen (HLA) class I protein that interacts with inhibitory receptors and is commonly overexpressed in various cancers, thereby establishing itself as an inhibitory checkpoint immune ligand. It is also expressed in trophoblast cells during pregnancy and protects the fetus from immune rejection. Despite its crucial role and its intriguing expression pattern, the regulation of HLA-G’s expression is only partially understood. HLA-G’s mRNA is expressed in many tissues but the protein expression is restricted only to the cells mentioned above. Therefore, we suggest that HLA-G is post-transcriptionally regulated. Here, we reveal a distinctive site present only in the 3′ Untranslated region (UTR) of HLA-G, which might explain its unique expression pattern. Consequently, we attempted to find binding factors such as RNA binding proteins (RBPs) and microRNAS (miRs) that regulate HLA-G expression by interacting with this distinct site present in its 3′ UTR. Our research indicates that this site should be further studied in order to reveal its significance.

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Section: Mir-1301 Is Not Involved In Hla-g Regulationsupporting

confidence: 79%

A Unique Regulation Region in the 3′ UTR of HLA-G with a Promising Potential

Reches

Berhani

Mandelboim

2020

IJMS

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“…IPA of our data revealed that more than 50 pathways were disrupted in the first-trimester placenta derived from IVF-ET. Further analysis showed that these pathways are widely involved in key events of early placental development and function, whose disruption can lead to adverse outcomes [47][48][49]. We report the enrichment of multiple genes in the top 50 significantly overrepresented biological pathways.…”

Section: Discussionmentioning

confidence: 74%

The placental transcriptome of the first-trimester placenta is affected by in vitro fertilization and embryo transfer

Zhao¹,

Zheng²,

Liu³

et al. 2019

Reprod Biol Endocrinol

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Background: The placenta is a highly specialized temporary organ that is related to fetal development and pregnancy outcomes, and epidemiological data demonstrate an increased risk of placental abnormality after in vitro fertilization and embryo transfer (IVF-ET). Methods: This study examines alterations in the transcriptome profile of first-trimester placentas from IVF-ET pregnancies and analyzes the potential mechanisms that play a role in the adverse perinatal outcomes associated with IVF-ET procedures. Four human placental villi from first-trimester samples were obtained through fetal bud aspiration from patients subjected to IVF-ET due to oviductal factors. An additional four control human placental villi were derived from a group of subjects who spontaneously conceived a twin pregnancy. We analyzed their transcriptomes by microarray. Then, RT-qPCR and immunohistochemistry were utilized to analyze several dysregulated genes to validate the microarray results. Biological functions and pathways were analyzed with bioinformatics tools. Results: A total of 3405 differentially regulated genes were identified as significantly dysregulated (> 2-fold change; P < 0.05) in the IVF-ET placenta in the first trimester: 1910 upregulated and 1495 downregulated genes. Functional enrichment analysis of the differentially regulated genes demonstrated that the genes were involved in more than 50 biological processes and pathways that have been shown to play important roles in the first trimester in vivo. These pathways can be clustered into coagulation cascades, immune response, transmembrane signaling, metabolism, cell cycle, stress control, invasion and vascularization. Nearly the same number of up-and downregulated genes participate in the same biological processes related to placental development and maintenance. Procedures utilized in IVF-ET altered the expression of first-trimester placental genes that are critical to these biological processes and triggered a compensatory mechanism during early implantation in vivo. Conclusion: These data provide a potential basis for further analysis of the higher frequency of adverse perinatal outcomes following IVF-ET, with the ultimate goal of developing safer IVF-ET protocols.

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“…Kim et al found that with the growth of the adult myocardium, the expression of WEE1 did not change but that expression inhibition occurred as a result of myocardial ischemia and hypoxic damage and further activated the functional activity of cyclin [ 24 , 26 ]. KRBOX1-AS1 is a recently discovered lncRNA, and its function has been mainly reported to be associated with to the early development of severe early-onset eclampsia and preoperative chemotherapy responses in locally advanced rectal cancer [ 27 , 28 ]. Ha et al found that the KRBOX1-AS1 has been reported to play a key role in the proliferation and apoptosis of rectal cancer cells and thus affects preoperative radiation therapy in rectal cancer patients [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Analysis of Differential Immunocyte Infiltration and Potential ceRNA Networks Involved in the Development of Atrial Fibrillation

Deng

Chen

et al. 2020

BioMed Research International

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This study is aimed at identifying potential molecular mechanisms and candidate biomarkers in the left atrial regions for the diagnosis and treatment of valvular atrial fibrillation (VAF). Multibioinformatics methods, including linear models for microarray analysis (LIMMA), an SVA algorithm, CIBERSORT immune infiltration, and DNA methylation analysis, were employed. In addition, the protein-protein interaction (PPI) network, Gene Ontology (GO), and molecular pathways of differentially expressed genes (DEGs) or differential methylation regions were constructed. In all, compared with the normal rhythm group, 243 different mRNAs (29 downregulated and 214 upregulated) and 26 different lncRNAs (3 downregulated and 23 upregulated) were detected in the left atrium (LA) of atrial fibrillation (AF) patients, and the neutrophil and CD8+ T cell were infiltrated. Additionally, 199 different methylation sites (107 downregulated and 92 upregulated) were also identified based on DNA methylation analysis. After integration, ELOVL2, CCR2, and WEE1 were detected for differentially methylated and differentially transcribed genes. Among them, WEE1 was also a core gene identified by the competing endogenous RNA (ceRNA) network that included WEE1-KRBOX1-AS1-hsa-miR-17-5p, in VAF left atrial tissue. We combined the DNA methylation and transcriptional expression differential analysis and found that WEE1 (cg13365543) may well be a candidate gene regulated by DNA methylation modification. Moreover, KRBOX1-AS1 and WEE1 can compete endogenously and may mediate myocardial tissue infiltration into CD8+ T cells and participate in the AF process.

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Intrauterine growth restriction and placental gene expression in severe preeclampsia, comparing early-onset and late-onset forms

Abstract: There are significant differences in placental gene expression between severe early- and late-onset preeclampsia when both are associated with intrauterine growth restriction. ABI3BP, C7, HLA-G and IL2RB might contribute to the development of early form of severe preeclampsia.

Cited by 37 publications

References 42 publications

A Unique Regulation Region in the 3′ UTR of HLA-G with a Promising Potential

A Unique Regulation Region in the 3′ UTR of HLA-G with a Promising Potential

The placental transcriptome of the first-trimester placenta is affected by in vitro fertilization and embryo transfer

Comprehensive Analysis of Differential Immunocyte Infiltration and Potential ceRNA Networks Involved in the Development of Atrial Fibrillation

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