INTRASPECIFIC VARIATION OF NUCLEAR DNA CONTENT IN PINUS RESINOSA AIT.

Aegilops squarrosa (n=7) is the donor of the third or D genome to common wheat and also the donor of the pivotal genome to five polyploid species of section Vertebrata of the genus Aegilops.This diploid species grows as a predominantly autogamous wild grass or weed in the Middle East and is classified by ear morphology into two subspecies, ssp. eu-squarrosa and ssp. strangulate.The former includes three varieties, typica, anathera and meyeri and the latter only strangulate.A comprehensive investigation in respect to morphology, genetics, cytology, physiology, susceptibility to rust and distribu tion of this species was reported by Kihara et al. (1965). Ae. squarrosa as well as Triticum monococcum and Ae. speltoides constitute a large unexplored gene pool for wheat breeding (Zohary et al. 1969). The practical method of introducing agriculturally important genetic variability into common wheat from Ae. squarrosa has already been established (Kerber and Dyck 1969).Recently, Furuta et al. (1974) confirmed a considerable intraspecific difference in nuclear DNA content among four varieties of Ae. squarrosa.In order to estimate the amount of genetic variation within species in relation to phylogeny of polyploid wheat, we carried out cytophotometrical masurements of nuclear DNA content with 27 strains of Ae. squarrosa. MATERIALS AND METHODSVarieties and sources of 27 strains used in this study are listed in Table 1, and the collection sites are shown in Fig. 1 Young spikes at the meiotic stage were fixed with modified Carnoy's fluid (glacial acetic acid 1: chloroform 3: 95 % ethanol 3) for three days and preserved in 75 % ethanol in a refrigerator.Anthers at the pollen tetrad stage of the standard (KUSE 2135-3) and of four other strains were smeared side by side on the cover slips.Subsequently, the pollen tetrads were dried, hydrolyzed with 1N HCl at 60°C for five minutes and stained by Schiff's reagent (Furuta 1975). Ten nuclei of each strain were 1) Contribution from the

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“…On the other hand, some species of Picea and Pinus (Miksche 1968, Dhir and Miksche 1974 and Vicia (Chooi 1971) showed variability in DNA content.…”

Section: Resultsmentioning

confidence: 99%

INTRASPECIFIC VARIATION OF NUCLEAR DNA CONTENT IN AEGILOPS SQUARROSA L.

Furuta

Nishikawa

Makino

1975

The Japanese journal of genetics

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“…Bennett and Smith (1971) found neither intraspecific nor interspecific variation in DNA content of diploid species of Hordeum. Intraspecific variation of nuclear DNA content was reported in Picea glauca, P, sitchensis, Pinus banksiana and P. resinosa (Miksche 1968(Miksche , 1971Dhir and Miksche 1974), while in the recent study by Teoh and Rees (1976) such variation was not confirmed in Picea glauca, P. engelmamii and Pinus contorta.…”

Section: Resultsmentioning

confidence: 75%

Uniformity of nuclear DNA content in Triticum monococcum L.

Furuta

Nishikawa

Haji

1978

The Japanese journal of genetics

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“…Xiaoxingan in Northeast, China. Chromosome number of this species was 2n=24 in correspondence with other species in the genus Pinus, but there were some reports showing that considerable intraspecific and interspecific variation of nuclear DNA content in this genus (Miksche 1968, 1971, Price et al 1973, Dhir and Miksche 1974, Teoh and Rees 1976, Dhillon 1980, Dhillon et al 1978, Ohri and Khoshoo 1986, Wakamiya et al 1993, Wyman et al 1997. Ohri and Khoshoo (1986) observed that the 20 Pinus species widely collected from North America, Europe and Eurasia had a 1.73-fold variation in nuclear DNA content, and noted that Pinus species with very high DNA content distributed in temperate and highly xeric habitats.…”

mentioning

confidence: 71%

“…The ratio of the highest 1C nuclear DNA content to the lowest 1C nuclear DNA content was 1.36, which was lower than the ratio of 1.5, 1.6, 1.7 and 1.9 respectively among the provenances of Pinus banksiana, Picea glauca, Picea sitchensis and Pseudotsuga menziesii (Miksche 1968, 1971, El-Lakany and Sziklai 1971. Dhir and Miksche (1974) observed that the maximum ratio was 2.2 of North red pine (Pinus resinosa) distributed in relatively small geographical distribution. Price (1988) considered that difference in total nuclear content was in range of 5-10%, it was sufficient to be subject to selection.…”

Section: Resultsmentioning

confidence: 99%

1C Nuclear DNA Content in Pinus koraiensis in Heilongjiang Province, China.

Liu

1998

CYTOLOGIA

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Summary In this study, 1C nuclear DNA content of Pinus koraiensis collected from different sample areas was reported. Mean 1C nuclear DNA content of this species was 24.60 pg. Plants with higher 1C nuclear DNA content distributed in southern, southwestern and northeastern slopes and tableland, those with lower 1C nuclear DNA content in western slope and mountain spur . Climatic factors may be important factors related to genome size of Pinus koraiensis.Pinus koraiensis is an important tree species, distributed in Mts . Changbaishan, Mts. Wandashan and Mts. Xiaoxingan in Northeast, China. Chromosome number of this species was 2n=24 in correspondence with other species in the genus Pinus, but there were some reports showing that considerable intraspecific and interspecific variation of nuclear DNA content in this genus (Miksche 1968, 1971, Price et al. 1973, Dhir and Miksche 1974, Teoh and Rees 1976, Dhillon 1980, Dhillon et al. 1978, Ohri and Khoshoo 1986, Wakamiya et al. 1993, Wyman et al. 1997. Ohri and Khoshoo (1986) observed that the 20 Pinus species widely collected from North America, Europe and Eurasia had a 1.73-fold variation in nuclear DNA content, and noted that Pinus species with very high DNA content distributed in temperate and highly xeric habitats. Recently , negative correlations were observed between nuclear DNA content and two climatic factors among 18 North American Pinus species studied by Wakamiya et al. (1993). In Pinus, a constancy of nuclear DNA content was found in different seed sources of P. taede, P. mugo, P. contorta and P. wallichiana, especially populations of P. wallichiana from rather different habits (one from an extremely dry area with annual precipitation of 10 to 20 in. and the other from a wet region with annual precipitation of up to 200 in.) (Teoh and Rees 1976, Ohri and Khoshoo 1968, Wakamiya et al. 1993. In sharp contrast to those Pinus species, the increase in nuclear content of Pinus banksiana, Picea glauca, Picea sitchenisi and Pseudostuga meziesii showed a clinical pattern from south to north in the geographic area of species (Miksche 1968, 1971, El-Lakany and Sziklai 1971.It has become evident that nuclear DNA content varies considerably not only among species , but also among and within populations or cultivars of a single species (Bennett and Leitch 1995). This variation in nuclear DNA content was showed to be developmental and adaptive important in life history of plant species via effects on parameters such as nuclear volume, cell volume, mitotic cycle time and duration of meiosis etc. (Bennett 1972, 1985, 1987, Kenton et al . 1986, Cavallini et al. 1993. Being an important tree species, nuclear DNA content of Pinus koraiensis was unknown. Hence, this study first reported nuclear DNA content of P. koraiensis from different sample areas in Liangshui Korean Pine Conservation District in Mts. Xiaoxingan, Heilongjiang, in order to understand relations of nuclear DNA content with growth rate, habit and generation time as well as relations with ecological or envir...

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INTRASPECIFIC VARIATION OF NUCLEAR DNA CONTENT IN PINUS RESINOSA AIT.

Cited by 40 publications

References 16 publications

INTRASPECIFIC VARIATION OF NUCLEAR DNA CONTENT IN <i>AEGILOPS SQUARROSA</i> L.

INTRASPECIFIC VARIATION OF NUCLEAR DNA CONTENT IN <i>AEGILOPS SQUARROSA</i> L.

Uniformity of nuclear DNA content in Triticum monococcum L.

1C Nuclear DNA Content in Pinus koraiensis in Heilongjiang Province, China.

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