Intraspecific genetic variation in Heterobasidion annosum revealed by amplification of minisatellite DNA

Stenlid, Jan; Karlsson, Jens; Högberg, Nils

doi:10.1016/s0953-7562(09)80337-7

Cited by 183 publications

(124 citation statements)

References 25 publications

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“…OTU homogeneity was verified by using fingerprints amplified with the M13 minisatellite primer (5 -GAGGGTGGCGGTTCT-3 ) [27]. The PCR reaction was performed in a total volume of 50 µL, and it consisted of the following steps: 10 min at 93 • C followed by 45 cycles of 20 s at 93 • C, 1 min at 48 • C, 20 s at 72 • C and a final incubation step of 6 min at 72 • C. Furthermore, molecular identification of each OTU was based on rDNA sequencing (i.e., ITS region), which was amplified by using ITS1F (5 -CTTGGTCATTTAGAGGAAGTAA-3 ) and ITS4 (5 -TCCTCCGCTTATTGATATGC-3 ) as a primer pair [28].…”

Section: Fungal Isolation and Identificationmentioning

confidence: 99%

Sydowia polyspora Dominates Fungal Communities Carried by Two Tomicus Species in Pine Plantations Threatened by Fusarium circinatum

Muñoz‐Adalia

Sanz-Ros

Flores-Pacheco

et al. 2017

Forests

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Bark beetles (Coleoptera, Scolytinae) carry a diverse filamentous fungal community sometimes acting as vectors or carriers of phytopathogens. In this study, mycobiota carried by two Tomicus species (Tomicus piniperda and Tomicus destruens) were investigated through (i) morphological and molecular identification of taxa; (ii) taxonomic richness, diversity, evenness, dominance and phoresy indices; (iii) ecological network analysis and (iv) statistical co-occurrence analysis. The studied mycobiota were formed by eleven taxa and showed a moderate fungal diversity with low evenness. The fungus Sydowia polyspora was significantly abundant and dominated the community. All the fungal taxa were randomly associated. Both insect species (T. piniperda and T. destruens) were collected from plantations of Pinus radiata infected by Fusarium circinatum. The ecological factors that could drive community ecology and phoretic links between fungi and bark beetles are discussed.

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Section: Fungal Isolation and Identificationmentioning

confidence: 99%

Sydowia polyspora Dominates Fungal Communities Carried by Two Tomicus Species in Pine Plantations Threatened by Fusarium circinatum

Muñoz‐Adalia

Sanz-Ros

Flores-Pacheco

et al. 2017

Forests

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“…Several studies have examined the genetic structure of basidiomycete fungal populations (Stenlid, Karlsson & Högberg 1994, Högberg, StenUd & Karlsson 1995 Corresponding author.…”

Section: Introductionmentioning

confidence: 99%

“…Results from studies of mushroom fungi indicated only modest genetic divergence among populations separated by 10s or 100s of km (Stenlid et al 1994, Högberg et al 1995, Boisselier-Dubayle et al 1996, Saville et al 1996, James et al 1999, suggesting that dispersal capabihties of these species may be sufficient to mitigate the isolating effects of habitat fragmentation.…”

Section: Introductionmentioning

confidence: 99%

Population genetic structure of the polypore Datronia caperata in fragmented mangrove forests

Parrent

Garbelotto

Gilbert

2004

Mycological Research

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“…RAPD analysis was carried out using three different primers: Coc1 (59-AGCAGCGTGG-39) (Cocconcelli et al, 1995), M13 (59-GAGGGTGGCGGTTCT-39) (Stendid et al, 1994) and D8635 (59-GAGCGGCCAAAGGGAGCAGAC-39) (Akopyanz et al, 1992), as described in the respective references except for the addition of 5 % (v/v) DMSO in each reaction mixture. Pattern analyses of the amplification products, separated in agarose gels and stained with ethidium bromide, were performed with the GELCOMPAR software (Applied Maths) with the DICE coefficient and UPGMA as calculation and clustering options.…”

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confidence: 99%

Lactobacillus delbrueckii subsp. indicus subsp. nov., isolated from Indian dairy products

Dellaglio

Felis

Castioni

et al. 2005

International Journal of Systematic and Evolutionary Microbiology

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Four strains isolated from Indian dairy products and initially identified as Lactobacillus delbrueckii could not be assigned to a definite subspecies because molecular identification and phenotypic traits did not agree with those of recognized subspecies of L. delbrueckii. Hybridization of total DNA (78-86 % against type strains of the other three subspecies), AFLP and RAPD-PCR fingerprints, phylogenetic analysis based on 16S rRNA gene sequences and sequence analysis of two coding genes (recA and hsp60), together with phenotypic profiles, indicated that the four strains form a coherent cluster and represent a novel subspecies, for which the name Lactobacillus delbrueckii subsp. indicus subsp. nov. is proposed. The type strain is NCC725 T (=LMG 22083 T =DSM 15996 T ).Lactobacillus delbrueckii is the type species of the genus Lactobacillus and comprises three subspecies, Lactobacillus delbrueckii subsp. delbrueckii, Lactobacillus delbrueckii subsp. lactis and Lactobacillus delbrueckii subsp. bulgaricus (Weiss et al., 1983). Strains belonging to the three subspecies show a high degree of DNA-DNA relatedness but can be differentiated on the basis of different phenotypic traits (Weiss et al., 1983;Kandler & Weiss, 1989).In a previous study regarding the evolutionary structure of the species L. delbrueckii (Germond et al., 2003), a number of lactobacilli originating from five dairy fermented products from India were analysed. Among those lactobacilli, four strains isolated from traditional Indian dairy fermented (type Dahi) products were identified as L. delbrueckii on the basis of molecular and phenotypic evidence, i.e. hybridization with a probe specific for L. delbrueckii, pY85 (Delley et al., 1990), typing based on digested genomic DNA probed with ribosomal or ISL7 sequences, specific PCR amplification and the exclusive production of D-lactate from lactose. However, they could not be assigned to one of the known subspecies of L. delbrueckii because they presented atypical characteristics such as the ability of L. delbrueckii subsp. bulgaricus to degrade lactose constitutively, but no amplification of the gene encoding b-galactosidase using specific primers for L. delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis.We report here the polyphasic analysis performed to define the taxonomic position of the four strains and describe a novel subspecies for L. delbrueckii.The bacterial strains NCC725 T , NCC665, NCC757 and NCC780 and the type strains of L. delbrueckii subsp. delbrueckii NCC621 T (=ATCC 9649 T =LMG 6412 T ), L. delbrueckii subsp. lactis NCC946 T (=ATCC 12315 T =LMG 7942 T ) and L. delbrueckii subsp. bulgaricus NCC641 T (=ATCC 11842 T =LMG 6901 T ) were grown in de ManRogosa-Sharpe (MRS) or acidified MRS (pH 5?4) medium at 37 u C.Sugar metabolism was evaluated using the API-50CH detection kit. All strains tested fermented glucose, fructose and mannose. The ability to metabolize lactose distinguished the four strains from L. delbrueckii subsp. delbrueckii. These four strains can also be differenti...

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Intraspecific genetic variation in Heterobasidion annosum revealed by amplification of minisatellite DNA

Cited by 183 publications

References 25 publications

Sydowia polyspora Dominates Fungal Communities Carried by Two Tomicus Species in Pine Plantations Threatened by Fusarium circinatum

Sydowia polyspora Dominates Fungal Communities Carried by Two Tomicus Species in Pine Plantations Threatened by Fusarium circinatum

Population genetic structure of the polypore Datronia caperata in fragmented mangrove forests

Lactobacillus delbrueckii subsp. indicus subsp. nov., isolated from Indian dairy products

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