Intranuclear Incorporation and Binding to Chromosomal DNA of Epinephrine

Binding of epinephrine to chromatin components.

“…In addition to such physiological functions, CA show DNA-breaking activity in the presence of Cu2+ in vitro1"* and in cultured mammalian cells.2~4) It has been believed that CA express their functions through their binding to the cellular membrane and activation of adenyl cyclase.5) However, 14C-Ep added into culture media is incorporated quickly into rat fetal lung cells and about 50% of the incorporated Ep binds to DNA. 6) Moreover, the DNA-breaking activity of Ep correlates with the amounts of Ep bound to DNAin various cell lines.40 These data suggest that the Ep incorporated into cells attacks cellular DNA directly and induces DNA breakage. Thus, to clarify the mechanism of the DNA-breaking reaction by Ep in mammalian cells, we examinedthe interaction between Ep and chromatin componentssuch as DNAand histones.…”

Binding of Epinephrine to Chromatin Components

“…S ) However, 14C_ Ep added into culture media is incorporated quickly into rat fetal lung cells and about 50% of the incorporated Ep binds to DNA. 6 ) Moreover, the DNA-breaking activity of Ep correlates with the amounts of Ep bound to DNA in various celllines. 4 ) These data suggest that the Ep incorporated into cells attacks cellular DNA directly and induces DNA breakage.…”