Intrahepatic gene expression in human alcoholic hepatitis

Seth, Devanshi; Gorrell, Mark D.; Cordoba, Shaun; McCaughan, Geoffrey W.; Haber, Paul S.

doi:10.1016/j.jhep.2006.04.013

Cited by 67 publications

(61 citation statements)

References 34 publications

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“…Recent high-throughput technologies, such as micro arrays, genomics and proteomics have led to novel concepts in our understanding of several liver pathologies (Decaciuc et al, 2004;Seth et al, 2006). Application of these technologies has identified novel pathways that could not have been discovered using traditional methods and opened up several lines of investigation for understanding the mechanism involved in alcohol-mediated liver injury.…”

Section: Genetic Element Of Aldmentioning

confidence: 99%

“…Application of these technologies has identified novel pathways that could not have been discovered using traditional methods and opened up several lines of investigation for understanding the mechanism involved in alcohol-mediated liver injury. Hepatic gene profiling using DNA micro arrays are reported from animal models of ALD and human ALD (Decaciuc et al, 2004;Seth et al, 2006). The ALD transcriptome profile is dominated by alcohol metabolism and inflammation related molecules, thus differing from other liver diseases (Seth et al, 2003).…”

Section: Genetic Element Of Aldmentioning

confidence: 99%

See 1 more Smart Citation

The Role of Liver Transplantation in theTreatment of Alcoholic Liver Disease

Tsoulfas¹,

Agorastou²

2012

Trends in Alcoholic Liver Disease Research - Clinical and Scientific Aspects

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Section: Genetic Element Of Aldmentioning

confidence: 99%

Section: Genetic Element Of Aldmentioning

confidence: 99%

The Role of Liver Transplantation in theTreatment of Alcoholic Liver Disease

Tsoulfas¹,

Agorastou²

2012

Trends in Alcoholic Liver Disease Research - Clinical and Scientific Aspects

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“…28 Tissue was obtained with consent in accordance with Australian Medical Research Council guidelines after approval by the institutional ethics committee. …”

Section: Rna Extraction and Cdna Synthesis From Human Livermentioning

confidence: 99%

“…Differential expression of DDR1a mRNA was quantified using a modification of published methods. 28,29 All reactions were performed in duplicate using a sequence detector (Prism, model 7700; Applied Biosytems, Inc.), and were analyzed using sequence detector software (Prism, version.1.6.3; Applied Biosystems, Inc.).…”

Section: Real-time Rt-pcrmentioning

confidence: 99%

Discoidin Domain Receptor 1

Song

Shackel

Wang

et al. 2011

The American Journal of Pathology

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Discoidin domain receptor 1 (DDR1) is a receptor tyrosine kinase that binds and is activated by collagens. Transcriptional profiling of cirrhosis in human liver using a DNA array and quantitative PCR detected elevated mRNA expression of DDR1 compared with that in nondiseased liver. The present study characterized DDR1 expression in cirrhotic and nondiseased human liver and examined the cellular effects of DDR1 expression. mRNA expression of all five isoforms of DDR1 was detected in human liver, whereas DDR1a demonstrated differential expression in liver with hepatitis C virus and primary biliary cirrhosis compared with nondiseased liver. In addition, immunoblot analysis detected shed fragments of DDR1 more readily in cirrhotic liver than in nondiseased liver. Inasmuch as DDR1 is subject to protease-mediated cleavage after prolonged interaction with collagen, this differential expression may indicate more intense activation of DDR1 protein in cirrhotic compared with nondiseased liver. In situ hybridization and immunofluorescence localized intense DDR1 mRNA and protein expression to epithelial cells including hepatocytes at the portal-parenchymal interface and the luminal aspect of the biliary epithelium. Overexpression of DDR1a altered hepatocyte behavior including increased adhesion and less migration on extracelular matrix substrates. DDR1a regulated extracellular expression of matrix metalloproteinases 1 and 2. These data elucidate DDR1 function pertinent to cirrhosis and indicate the importance of epithelial cell-collagen interactions in chronic liver injury.

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“…The effect of alcohol on RNA-gene expression has been studied in liver biopsies of patients with alcoholic steatosis, alcoholic hepatitis, alcoholic cirrhosis, end stage alcohol-induced liver disease, and non-diseased control individuals. These genes have also been studied in the LiberDeCarli baboon model [103,104]. Osteopontin, a Th1 cytokine and Opn receptors (integrin alpha v and CD44), were up-regulated in relation to the severity of liver disease.…”

Section: Co-morbidities Associated With Hcvmentioning

confidence: 99%

Inflammation and Repair in Viral Hepatitis C

et al. 2007

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Hepatitis C viral infection (HCV) results in liver damage leading to inflammation and fibrosis of the liver and increasing rates of hepatic decompensation and hepatocellular carcinoma (HCC). However, the host's immune response and viral determinants of liver disease progression are poorly understood. This review will address the determinants of liver injury in chronic HCV infection and the risk factors leading to rapid disease progression. We aim to better understand the factors that distinguish a relatively benign course of HCV from one with progression to cirrhosis. We will accomplish this task by discussion of three topics: (1) the role of cytokines in the adaptive immune response against the HCV infection; (2) the progression of fibrosis; and (3) the risk factors of co-morbidity with alcohol and human immunodeficiency virus (HIV) in HCV-infected individuals. Despite recent improvements in treating HCV infection using pegylated interferon alpha (PEGIFN-alpha) and ribavirin, about half of individuals infected with some genotypes, for example genotypes 1 and 4, will not respond to treatment or cannot be treated because of contraindications. This review will also aim to describe the importance of IFN-alpha-based therapies in HCV infection, ways of monitoring them, and associated complications.

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Intrahepatic gene expression in human alcoholic hepatitis

Cited by 67 publications

References 34 publications

The Role of Liver Transplantation in theTreatment of Alcoholic Liver Disease

The Role of Liver Transplantation in theTreatment of Alcoholic Liver Disease

Discoidin Domain Receptor 1

Inflammation and Repair in Viral Hepatitis C

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