Intragenomic variation in the second internal transcribed spacer of the ribosomal DNA of species of the genera Culex and Lutzia (Diptera: Culicidae)

Vesgueiro, Fabiana Tavares; Demari-Silva, Bruna; Malafronte, Rosely dos Santos; Sallum, Maria Anice Mureb; Marrelli, Mauro Toledo

doi:10.1590/s0074-02762011000100001

Cited by 30 publications

(31 citation statements)

References 32 publications

(33 reference statements)

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“…The length and GC content of this spacer for Cx. thieleri (315.5 bp and 59.09 %, in average) fit with the length and GC % obtained in other Culex species (271-339 bp and 52.8-57.5%) [24,[51][52][53]. The intra-specific variability observed in the ITS-2 sequences of Cx.…”

Section: Discussionsupporting

confidence: 77%

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Molecular Characterization of Culex Theileri from Canary Islands, Spain, a Potential Vector of Dirofilaria Immitis

Bargues¹

2013

J Clin Exp Pathol

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Dirofilaria immitis is the causal agent of heartworm diseases and of human pulmonary dirofilariosis. The infection is transmitted by several species of culicid mosquitoes that are frequently able to bite both animal reservoirs and humans. Canary Islands (Spain) constitute a well documented endemic area of canine dirofilariosis in which the mosquito species involved in the transmission of D. immitis are not known. The objectives of the present work were the identification of vectors of this parasite in Canary Islands and their molecular characterization. A total of 1219 female mosquitoes were captured. The most abundant species was Culex theileri (52.26%) followed by Cx. pipiens (35.44%), Anopheles cinereus hispaniola (6.23%), Culiseta longiareolata (5.74%), and Culex laticintus (0.33%). PCR was applied for the detection of larval D. immitis DNA in mosquitoes. D. immitis DNA was observed in the abdomen of one Cx. theileri female: 0.082% of the entire mosquito population and 0.17% in Cx. theileri. A molecular identification of Cx. theileri, the potential mosquito vector of dirofilariosis in this zoonotic focus in Canary Islands of Spain, has been made for first time based on sequences of the 18S rRNA gene, the second internal transcribed spacer (ITS2) of ribosomal DNA and the barcode region of the cytochrome c oxidase I (cox1) gene of mitochondrial DNA, allowing a broad mosquito molecular basis for future populations genetic analyses of this vector species. Parasitological and entomological molecular results suggest that Cx. theileri is a potential natural vector of D. immitis in Canary Islands.

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Section: Discussionsupporting

confidence: 77%

“…theileri in Canary Islands are similar to the heterogeneity observed between sequences of the same individual in different populations of the Cx. pipiens complex [54], as well as with the heterogeneity observed in individuals of the same species of Culex and Lutzia from Brazil [53].…”

Section: Discussionmentioning

confidence: 77%

Molecular Characterization of Culex Theileri from Canary Islands, Spain, a Potential Vector of Dirofilaria Immitis

Bargues¹

2013

J Clin Exp Pathol

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“…In subsequent years, several species were described and their similarities to Cx. coronator were observed [2][3][4][5][6][7][8]. Later, only Cx.…”

Section: Referências Bibliográficasmentioning

confidence: 99%

Caracterização de populações de Culex coronator (Diptera: Culicidae) e distinção de fêmeas adultas de Culex coronator e Culex usquatus por meio da análise da morfometria geométrica de asa e de sequências gênicas

Silva¹

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Caracterização de populações de 2014É expressamente proibida a comercialização deste documento tanto na sua forma impressa como eletrônica. Sua reprodução total ou parcial é permitida exclusivamente para fins acadêmicos e científicos, desde que na reprodução figure a identificação do autor, título, instituição e ano da tese. À minha mãe pelo amor e dedicação, ao meu pai e ao meu irmão, meus biólogos queridos, pelos ensinamentos e apoio. usquatus was recorded only in Central and South America. Therefore, these species are sympatric in Brazil. Despite the morphological similarity of the females of both species, only Cx. coronator has epidemiological importance, being found infected with many arboviruses. Since studies focusing in population structure are important to understand the evolution and dynamics of potencial vectors and that the correct female identification is critical for development of vectorial competence studies, the aims of this study were to: (1) distinguish adult females of Cx. coronator from Cx. usquatus (2) obtain knowledge of the population structure of Culex coronator in Southern and Southeastern areas (3) examine the presence of undescribed and/or incorrectly identified species under Cx. coronator. Thereby, a survey was carried out using morphological (wing geometry) and genetic (4 microsatellite loci and barcode region of the Cytochrome c oxidase subunit I gene) markers. Results showed genetic and morphological diversity of southeastern populations, with low population structure, while southern populations were more homogenous but different from those of Southeast Brazil. Thus, analysis of microsatellite and wing morphometry showed some populational structure according to South DEDICO AGRADECIMENTOS

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“…Marcadores moleculares têm sido amplamente utilizados em estudos filogenéticos, na resolução de questões taxonômicas e estudos de genética de população de vários grupos de insetos (Monteiro et al 2000;Vesgueiro et al 2011;Volkman et al 2012;Wang et al 2012 (Wright 1978).…”

Section: Marcadores Molecularesunclassified

“…A utilização de rDNA provou ser de muita utilidade para resolver problemas ligados a relações evolutivas em diferentes níveis taxonômicos (Vesgueiro et al 2011). …”

Section: Marcadores Molecularesunclassified

Culex quinquefasciatus (Diptera: Culicidae): aspectos da manipulação genética e estudos populacionais utilizando marcadores microssatélites

Wilke¹

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Intragenomic variation in the second internal transcribed spacer of the ribosomal DNA of species of the genera Culex and Lutzia (Diptera: Culicidae)

Cited by 30 publications

References 32 publications

Molecular Characterization of Culex Theileri from Canary Islands, Spain, a Potential Vector of Dirofilaria Immitis

Molecular Characterization of Culex Theileri from Canary Islands, Spain, a Potential Vector of Dirofilaria Immitis

Caracterização de populações de Culex coronator (Diptera: Culicidae) e distinção de fêmeas adultas de Culex coronator e Culex usquatus por meio da análise da morfometria geométrica de asa e de sequências gênicas

Culex quinquefasciatus (Diptera: Culicidae): aspectos da manipulação genética e estudos populacionais utilizando marcadores microssatélites

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