Intradermal Test in American Leishmaniasis with a Polysaccharide Fraction Isolated from Leishmania Brasilensis1

Immunology of American Leishmaniasis

“…Senekji's'is observation that polysaccharide antigen of L. tropica gave constant specific results in oriental sore caused us to use this fraction in American leishmaniasis. 21 The test was performed in 21 patients and 45 controls (10 normal individuals and 35 with miscellaneous tropical dermatoses). The positivity with tlie polysaccharide antigen was 86 per cent, coiiiparable with the results of the tests obtained with a susjJension of 10,000,000 leplomonads per ml, caiTied out in the same group.…”

Evaluation and characterization of partially purified skin test antigens prepared from Leishmania panamensis promastigotes.

“…Although the antigen commonly used is a suspension of whole parasites in phenolized saline (Buss, 1929) , a little information on other preparations prepared from promastigote-extracts have been also reported (Furtado and Pellegrino, 1956;La Placa et al, 1975;Shaw and Lainson, 1975;Reed et al, 1986) . Among them, Reed et al (1986) have reported that a crude soluble extract prepared from ruptured Leishmania chagasi promastigotes was highly sensitive and specific for the diagnosis of American visceral leishmaniasis .…”