Intracellular Trafficking of Magnetic Nanoparticles to Design Multifunctional Biovesicles

Wilhelm, Claire; Lavialle, Françoise; Péchoux, Christine; Tatischeff, Irène; Gazeau, Florence

doi:10.1002/smll.200700523

Cited by 55 publications

(39 citation statements)

References 24 publications

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“…TEM images of the loaded silicon particles are shown in figure 4D. The SPIONs were selected based on earlier findings that indicated endosomal sorting of positive SPIONs from carrier silicon particles18, and reports that different particle coatings are known to promote endosomal escape of nanoparticles, including polyethyleneimine (PEI) 19, poly-lactic- co -glycolic acid (PLGA)20 and chitosan 21, 22.…”

Section: Resultsmentioning

confidence: 99%

Intracellular trafficking of silicon particles and logic-embedded vectors

et al. 2010

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Mesoporous silicon particles show great promise for use in drug delivery and imaging applications as carriers for second-stage nanoparticles and higher order particles or therapeutics. Modulation of particle geometry, surface chemistry, and porosity allows silicon particles to be optimized for specific applications such as vascular targeting and avoidance of biological barriers commonly found between the site of drug injection and the final destination. In this study, the intracellular trafficking of unloaded carrier silicon particles and carrier particles loaded with secondary iron oxide nanoparticles was investigated. Following cellular uptake, membrane-encapsulated silicon particles migrated to the perinuclear region of the cell by a microtubule-driven mechanism. Surface charge, shape (spherical and hemispherical) and size (1.6 and 3.2 μm) of the particle did not alter the rate of migration. Maturation of the phagosome was associated with an increase in acidity and acquisition of markers of late endosomes and lysosomes. Cellular uptake of iron oxide nanoparticle-loaded silicon particles resulted in sorting of the particles and trafficking to unique destinations. The silicon carriers remained localized in phagosomes, while the second stage iron oxide nanoparticles were sorted into multi-vesicular bodies that dissociated from the phagosome into novel membrane-bound compartments. Release of iron from the cells may represent exocytosis of iron oxide nanoparticleloaded vesicles. These results reinforce the concept of multi-functional nanocarriers, in which different particles are able to perform specific tasks, in order to deliver single-or multi-component payloads to specific sub-cellular compartments.

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Section: Resultsmentioning

confidence: 99%

Intracellular trafficking of silicon particles and logic-embedded vectors

et al. 2010

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“…Superparamagnetism is the term used for describing the absence of coercivity and remanent magnetization in particles that still maintain a considerable amount of polarizable spins under the effect of an external magnetic field [36,37]. Due to their small sizes and their zero net magnetization at zero field, they can be safely exploited in biomedicine, for instance as nanovectors for specific targets that are not accessible via other conventional approaches [33][34][35]. In the same way, they can also be used in biodetection and bioseparation techniques since once the target molecule has been attached to the nanocrystals, the application of an external magnetic field allows for their recovery [38,39].…”

Section: Introductionmentioning

confidence: 99%

“…Many of the application of magnetic nanoparticles are in life sciences and biomedicine [33][34][35]. Superparamagnetism is the term used for describing the absence of coercivity and remanent magnetization in particles that still maintain a considerable amount of polarizable spins under the effect of an external magnetic field [36,37].…”

Section: Introductionmentioning

confidence: 99%

Physical properties of elongated inorganic nanoparticles

et al. 2011

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“…The targeting strategy, tested with bacterially derived vesicles [25], by using bi-specific antibodies, with one arm recognizing a component of the vesicle surface, the other a cell surface receptor of the target cell, might also be applied to Dictyostelium nanovesicles. Another vectorization strategy could be developed by using Dictyostelium cells cultured in the presence of magnetic nanoparticles to engineer magnetic nanovesicles [28].…”

Section: Discussionmentioning

confidence: 99%

A New Biological Strategy for Drug Delivery: Eucaryotic Cell-Derived Nanovesicles

Tatischeff¹,

Alfsen²

2011

JBNB

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An efficient drug delivery is the prerequisite of the successful chemotherapeutic treatments of many human diseases. Despite a great number of approaches, the improvement of drug cell internalization remains an actual research challenge. We propose a new biological delivery system based on the extracellular vesicles released by a non-pathological eukaryotic microorganism, Dictyostelium discoideum. After a summary of the main characteristics of these extracellular vesicles, including of their lipid bilayer that appears as a good candidate for initiating membrane fusion, followed by delivery of their encapsulated drug, the capacity of these vesicles to convey drugs into human cells was demonstrated in vitro on two tumor cell lines, resistant leukaemia K562r and cervix carcinoma HeLa cells. A comparison with other extracellular vesicles, like exosomes or bacteria-derived particles, stresses the unique properties of Dictyostelium extracellular nanovesicles for drug delivery.

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Intracellular Trafficking of Magnetic Nanoparticles to Design Multifunctional Biovesicles

Cited by 55 publications

References 24 publications

Intracellular trafficking of silicon particles and logic-embedded vectors

Intracellular trafficking of silicon particles and logic-embedded vectors

Physical properties of elongated inorganic nanoparticles

A New Biological Strategy for Drug Delivery: Eucaryotic Cell-Derived Nanovesicles

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