Intracellular Thermal Probing Using Aggregated Fluorescent Nanodiamonds

Wu, Tian-Li; Chen, Xixi; Gong, Zhiyong; Yan, Jiahao; Guo, Jinghui; Zhang, Yao; Li, Yuchao; Li, Baojun

doi:10.1002/advs.202103354

Cited by 22 publications

(23 citation statements)

References 60 publications

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“…A small volume of cell suspension was taken for cell counting. Then, the cells were added to either FND-containing medium or FND-free DMEM-HG complete and seeded in quartered glass bottom Petri dishes at 1 x 10 4 cells/cm 2 (500 μL cell suspension per quarter). After 48 hours, in the butyrate-treated group, the medium was changed to a medium containing 5 mM sodium butyrate (Sigma-Aldrich).…”

Section: Methodsmentioning

confidence: 99%

“…At the same time, this extremely stable fluorescence can be modulated by external physical factors. The combination of these properties has led to the development of nanodiamond-based quantum sensing protocols, where the fluorescence of FNDs in the sample is monitored to obtain information on parameters as temperature 1,2 , pH 3 , or magnetic fields 4 . One of the most exciting research avenues is applying these sensing protocols to biological samples.…”

Section: Introductionmentioning

confidence: 99%

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Evolution of intracellular free radical load in colon adenocarcinoma cells over the course of butyrate-induced redifferentiation

Sigaeva

Zijlema

Zhang

et al. 2023

Preprint

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Fluorescent nanodiamonds have exceptional optical properties and are highly biocompatible, which allows to use them as labels for long-term tracking of the cells. The research fields that make use of this application of nanodiamonds include stem cell biology and cancer biology, where quiescent and differentiating cells can be traced in vitro and in vivo. However, these studies focus on using nanodiamonds as simple labels, whereas they can serve as highly sensitive intracellular sensors for free radical species. In this chapter, we aimed to bring the two approaches together and to assess the free radical production in the cells over the course of their differentiation. We report on the successful enterocytic differentiation of HT-29 colon adenocarcinoma cells, pre-loaded with fluorescent nanodiamonds. The cells were cultured in butyrate-free or butyrate-supplemented medium for 13 days. Butyrate-treated cells developed the morphological and molecular traits, characteristic for the normal enterocytes. Fluorescent nanodiamonds did not have a negative effect on the process of differentiation. Moreover, the particles could be found in the cytoplasm of both undifferentiated and re-differentiated cells even after 13 days of culture. The internalized nanodiamonds were used to assess the free radical load in the undifferentiated and re-differentiated HT-29 cells at different stages of the experiment. Consistently with previous findings, re-differentiated HT-29 cells showed higher free radical load than undifferentiated ones.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evolution of intracellular free radical load in colon adenocarcinoma cells over the course of butyrate-induced redifferentiation

Sigaeva

Zijlema

Zhang

et al. 2023

Preprint

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“…This method enables the construction and localization of nanoprobes within cells and has potential applications in high-precision, flexible singlecell analysis. 627…”

Section: Cnos Composite Sensorsmentioning

confidence: 99%

Section: Sensing and Imaging Applications Of Nds-based Sensorsmentioning

confidence: 99%

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Zero-Dimensional Carbon Nanomaterials for Fluorescent Sensing and Imaging

Yang,

Xu,

et al. 2023

Chem. Rev.

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Advances in nanotechnology and nanomaterials have attracted considerable interest and play key roles in scientific innovations in diverse fields. In particular, increased attention has been focused on carbon-based nanomaterials exhibiting diverse extended structures and unique properties. Among these materials, zero-dimensional structures, including fullerenes, carbon nano-onions, carbon nanodiamonds, and carbon dots, possess excellent bioaffinities and superior fluorescence properties that make these structures suitable for application to environmental and biological sensing, imaging, and therapeutics. This review provides a systematic overview of the classification and structural properties, design principles and preparation methods, and optical properties and sensing applications of zero-dimensional carbon nanomaterials. Recent interesting breakthroughs in the sensitive and selective sensing and imaging of heavy metal pollutants, hazardous substances, and bioactive molecules as well as applications in information encryption, super-resolution and photoacoustic imaging, and phototherapy and nanomedicine delivery are the main focus of this review. Finally, future challenges and prospects of these materials are highlighted and envisaged. This review presents a comprehensive basis and directions for designing, developing, and applying fascinating fluorescent sensors fabricated based on zero-dimensional carbon nanomaterials for specific requirements in numerous research fields.

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Intracellular Thermal Probing Using Aggregated Fluorescent Nanodiamonds

Chen

Gong

et al. 2021

Advanced Science

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Intracellular thermometry provides important information about the physiological activity of single cells and has been implemented using diverse temperature‐sensitive materials as nanoprobes. However, measuring the temperature of specific organelles or subcellular structures is challenging because it requires precise positioning of the nanoprobes. Here, it is shown that dispersed fluorescent nanodiamonds (FNDs) endocytosed in living cells can be aggregated into microspheres using optical forces and used as intracellular temperature probes. The aggregation of the FNDs and electromagnetic resonance between individual nanodiamonds in the microspheres lead to a sevenfold intensity enhancement of 546‐nm laser excitation. With the assistance of a scanning optical tweezing system, the FND microspheres can be precisely patterned and positioned within the cells. By measuring the fluorescence spectra of the microspheres, the temperatures at different locations within the cells are detected. The method provides an approach to the constructing and positioning of nanoprobes in an intracellular manner, which has potential applications in high‐precision and flexible single‐cell analysis.

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Intracellular Thermal Probing Using Aggregated Fluorescent Nanodiamonds

Cited by 22 publications

References 60 publications

Evolution of intracellular free radical load in colon adenocarcinoma cells over the course of butyrate-induced redifferentiation

Evolution of intracellular free radical load in colon adenocarcinoma cells over the course of butyrate-induced redifferentiation

Zero-Dimensional Carbon Nanomaterials for Fluorescent Sensing and Imaging

Intracellular Thermal Probing Using Aggregated Fluorescent Nanodiamonds

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