Intracellular signal transduction mechanisms of rat epididymal spermatozoa and their relationship to motility and metabolism

Armstrong, V. L.; Clulow, John; Murdoch, R. N.; Jones, R. C.

doi:10.1002/mrd.1080380113

Cited by 33 publications

(38 citation statements)

References 32 publications

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“…Pentoxifylline inhibits cAMP phosphodiesterase, thus increasing the intracellular cAMP concentration (Garbers et al 1971). A rise in cAMP concentration causes an increase in the cAMP-dependent processes of spermatozoa such as motility, capacitation and acrosome reaction in spermatozoa (Armstrong et al 1994, Aitken 1997). …”

Section: Introductionmentioning

confidence: 99%

Pentoxifylline added to freezing or post-thaw extenders does not improve the survival or in vitro fertilising capacity of boar spermatozoa

Gil¹,

Hernández²,

Roca³

et al. 2010

Reproduction

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This study evaluated whether pentoxifylline added to freezing and thawing extenders influenced the function of boar spermatozoa. In Experiment 1, pooled ejaculated sperm-rich fractions were frozen in 0.5 ml straws after dilution in extender supplemented with pentoxifylline to a final concentration of 0, 2, 4, 8, 16 or 32 mM. The addition of 4, 8, 16 and 32 mM pentoxifylline to the freezing extender significantly decreased the progressive and total motility of spermatozoa. The percentage of viable spermatozoa with intact acrosomes as well as the penetration rate and the efficiency of fertilisation were significantly lower in pentoxifylline-treated groups compared with the untreated control. In Experiment 2, a pool of three straws with 'good' post-thaw sperm quality parameters and another three straws with 'poor' sperm quality were diluted in extender with 0, 1, 2, 4, 8, 16 or 32 mM pentoxifylline. Post-thaw samples with both 'good' and 'poor' sperm quality with 0, 2, 4, 8 and 16 mM were used to assess IVF parameters. The addition of pentoxifylline to post-thaw extender did not improve the post-thaw motility or viability of spermatozoa compared with the control. The in vitro penetration was higher (P!0.05) than the control for oocytes fertilised with spermatozoa that were thawed and incubated in extender with 4, 8 and 16 mM pentoxifylline. However, no differences were observed in the efficiency of fertilisation. We conclude that pentoxifylline, as a supplement added to the freezing extender, has a deleterious effect and that it does not improve the survival or in vitro fertilising efficiency of frozen-thawed boar spermatozoa when added after thawing.

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Section: Introductionmentioning

confidence: 99%

Pentoxifylline added to freezing or post-thaw extenders does not improve the survival or in vitro fertilising capacity of boar spermatozoa

Gil¹,

Hernández²,

Roca³

et al. 2010

Reproduction

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“…There is general agreement that the cyclic nucleotide adenosine 3 ,5 -cyclic monophosphate (cAMP) plays a role in regulating sperm function, including motility and there is also evidence to suggest that an increase in cAMP is associated with activation of motility in spermatozoa from the cauda epididymidis of hamsters (Morton et al, 1974) and rats (Armstrong et al, 1994). Ca 2+ also activates motility in spermatozoa from the cauda epididymidis from both these species (Morton et al, 1974;Armstrong et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

“…Ca 2+ also activates motility in spermatozoa from the cauda epididymidis from both these species (Morton et al, 1974;Armstrong et al, 1994). …”

Section: Introductionmentioning

confidence: 99%

Motility activation and second messenger signalling in spermatozoa from rat cauda epididymidis

Wade¹,

Jones²,

Murdoch³

et al. 2003

Reproduction

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This study examined molecular mechanisms involved in the activation of motility in spermatozoa from the cauda epididymidis of rats. A 1.05-fold dilution of semen from the cauda epididymidis with 300 mmol sucrose l −1 did not activate motility in spermatozoa. Addition of dibutyryl cAMP, pentoxifylline or Ca 2+ to the sucrose activated motility in the short term (< 30-60 min). A fivefold dilution of semen from the cauda epididymidis with a modified Tyrode's medium (BWW) activated and sustained vigorous motility that could not be attenuated with kinase inhibitors. This motility was associated with a transient increase in intracellular cAMP during the first 60 s of activation. Lower motility was activated in Ca 2+ -deficient media but this was not associated with an increase in cAMP. A fivefold dilution with plasma from the cauda epididymidis did not activate motility. The addition of Ca 2+ to the sucrose induced an increase in cAMP of similar duration but lower magnitude to that associated with dilution in BWW. The results from this study indicate that the cAMP and Ca 2+ signal transduction pathways are involved in activation of sperm motility, and that the increase in intracellular cAMP in rat spermatozoa from the cauda epididymidis undergoing motility activation is Ca 2+ -dependent. This is the first study to report a Ca 2+ -dependent increase in cAMP associated with motility activation in immotile mammalian spermatozoa. In light of these data, a model is proposed whereby cAMP and Ca 2+ act as synarchic messengers, initiating a signal transduction cascade, which is independent of protein kinase A-mediated phosphorylation of flagella proteins in immotile spermatozoa from the cauda epididymidis.

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“…One possibility might be the involvement of cAMP signal transduction pathways. It has been reported that these pathways are involved in activation of sperm motility (Armstrong et al, 1994;Wade et al, 2003). This increase in motility is an important factor affecting fertilization, because high rates of ovum fertilization require efficient transport of sperm from the cervix to the oviducts (Hawk et al, 1978).…”

Section: Discussionmentioning

confidence: 99%

The effect of oestradiol-17β on the motility, viability and the acrosomal status of bull sperm

Çiftci

Zülkadir

2010

SA J. An. Sci.

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The aim of this study was to test the effect of oestradiol-17β (E 2 ) on the motility, viability and the acrosomal status of bull sperm in vitro. Pooled semen from Holstein bulls were incubated in the presence of 2, 4, and 8 μg E 2 /mL for 24 h. Semen was also incubated in media without E 2 . During the incubation, the number of motile, viable sperm and the number of sperm possessing lost/damaged acrosomes, cytoplasmic droplets and coiled tails were counted at 0, 4, 18 and 24 h of incubation. Addition of 2 μg E 2 /mL at 18 h of incubation increased the total motility over the control. The number of forward progressing sperm was increased by the supplementation of 2 and 8 μg E 2 /mL over the control group at the 4 h incubation. Lower doses of E 2 (2 and 4 μg/mL) did not affect viability of sperm, but a high dose of E 2 (8 μg/mL) caused reductions in viability at 4 and 24 h of incubation. The number of sperm cells with lost acrosomes was significantly high in control group at 24 h of incubation. The number of sperm cells possessing proximal and distal cytoplasmic droplets and the number of sperm cells bearing coiled tails were not altered by any of the treatments. A small dose of E 2 (2 μg/mL) had a beneficial effect on the motility and acrosome integrity of bull sperm in vitro. Higher dose of E 2 (8 μg/mL) had a detrimental effect on viability.

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Intracellular signal transduction mechanisms of rat epididymal spermatozoa and their relationship to motility and metabolism

Cited by 33 publications

References 32 publications

Pentoxifylline added to freezing or post-thaw extenders does not improve the survival or in vitro fertilising capacity of boar spermatozoa

Pentoxifylline added to freezing or post-thaw extenders does not improve the survival or in vitro fertilising capacity of boar spermatozoa

Motility activation and second messenger signalling in spermatozoa from rat cauda epididymidis

The effect of oestradiol-17β on the motility, viability and the acrosomal status of bull sperm

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