Intracellular Imaging with a Graphene‐Based Fluorescent Probe

Cheng, Peng; Hu, Wenbing; Zhou, Yuntao; Fan, Chunhai; Huang, Qing

doi:10.1002/smll.201000560

Cited by 260 publications

(187 citation statements)

References 47 publications

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“…This is, however, in accordance with previous findings, 77 where more than 87% HT-29 cell viability was observed for graphene. A similar finding was also observed for other types of cancer cells such as HeLa cancer cells, 78 where cell viability of 90% was observed for GO at 40 μg/mL. Miao et al 79 also observed about 87% cell viability of GO towards SCC7 cells.…”

supporting

confidence: 73%

Exceedingly biocompatible and thin-layered reduced graphene oxide nanosheets using an eco-friendly mushroom extract strategy

Muthoosamy

Bai

Abubakar

et al. 2015

IJN

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Purpose A simple, one-pot strategy was used to synthesize reduced graphene oxide (RGO) nanosheets by utilizing an easily available over-the-counter medicinal and edible mushroom, Ganoderma lucidum . Methods The mushroom was boiled in hot water to liberate the polysaccharides, the extract of which was then used directly for the reduction of graphene oxide. The abundance of polysaccharides present in the mushroom serves as a good reducing agent. The proposed strategy evades the use of harmful and expensive chemicals and avoids the typical tedious reaction methods. Results More importantly, the mushroom extract can be easily separated from the product without generating any residual byproducts and can be reused at least three times with good conversion efficiency (75%). It was readily dispersible in water without the need of ultrasonication or any surfactants; whereas 5 minutes of ultrasonication with various solvents produced RGO which was stable for the tested period of 1 year. Based on electrochemical measurements, the followed method did not jeopardize RGO’s electrical conductivity. Moreover, the obtained RGO was highly biocompatible to not only colon (HT-29) and brain (U87MG) cancer cells, but was also viable towards normal cells (MRC-5). Conclusion Besides being eco-friendly, this mushroom based approach is easily scalable and demonstrates remarkable RGO stability and biocompatibility, even without any form of functionalization.

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supporting

confidence: 73%

Exceedingly biocompatible and thin-layered reduced graphene oxide nanosheets using an eco-friendly mushroom extract strategy

Muthoosamy

Bai

Abubakar

et al. 2015

IJN

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“…60 However, when cancer cell lines were used, decreases in cell viability were lower in most cases. 59,65,66 There are a few in vivo studies with regard to the biocompatibility of graphene and its derivatives.…”

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confidence: 99%

Is Graphene a Promising Nano-Material for Promoting Surface Modification of Implants or Scaffold Materials in Bone Tissue Engineering?

Liu

Chen

et al. 2014

Tissue Engineering Part B: Reviews

101

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Bone tissue engineering promises to restore bone defects that are caused by severe trauma, congenital malformations, tumors, and nonunion fractures. How to effectively promote the proliferation and osteogenic differentiation of mesenchymal stem cells (MSCs) or seed cells has become a hot topic in this field. Many researchers are studying the ways of conferring a pro-osteodifferentiation or osteoinductive capability on implants or scaffold materials, where osteogenesis of seed cells is promoted. Graphene (G) provides a new kind of coating material that may confer the pro-osteodifferentiation capability on implants and scaffold materials by surface modification. Here, we review recent studies on the effects of graphene on surface modifications of implants or scaffold materials. The ability of graphene to improve the mechanical and biological properties of implants or scaffold materials, such as nitinol and carbon nanotubes, and its ability to promote the adhesion, proliferation, and osteogenic differentiation of MSCs or osteoblasts have been demonstrated in several studies. Most previous studies were performed in vitro, but further studies will explore the mechanisms of graphene's effects on bone regeneration, its in vivo biocompatibility, its ability to promote osteodifferentiation, and its potential applications in bone tissue engineering.

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“…6(i)). 122 Sun et al reported synthesis of IRDye800-conjugated GO loaded with VEGF as a targeted°uorescent probe for imaging of ischemic muscles in a murine model. 123 In addition to GO and rGO, another class of graphene-based nanomaterials have gained a great deal of attention in the recent years especially in the¯eld of biomedical imaging.…”

Section: Fluorescence Imagingmentioning

confidence: 99%

Graphene-Based Nanomaterials for Theranostic Applications

Roy

Jaiswal

2017

Rep. Adv. Phys. Sci.

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Graphene and graphene-based nanomaterials such as graphene oxide (GO), reduced graphene oxide (rGO) and graphene quantum dots (GQDs) have gained a lot of attention from diverse scienti¯c¯elds for applications in sensing, catalysis, nanoelectronics, material engineering, energy storage and biomedicine due to its unique structural, optical, electrical and mechanical properties. Graphene-based nanomaterials emerge as a novel class of nanomedicine for cancer therapy for several reasons. Firstly, its structural properties like high surface area and aromaticity enables easy loading of hydrophobic drugs. Secondly, presence of oxygen containing functional groups improve its physiological stability and also act as site for biofunctionalization. Thirdly, its optical absorption in the NIR region enable them to act as photoagents for photothermal and photodynamic therapies of cancer, both in vitro and in vivo. Finally, its intrinsic°u orescence property helps in bioimaging of cancer cells. Overall, graphene-based nanomaterials can act as agents for developing multifunctional theranostic platforms for carrying out more e±cient detection and treatment of cancers. This review provides a detailed summary of the di®erent applications of graphene-based nanomaterials in drug delivery, nucleic acid delivery, phototherapy, bioimaging and theranostics.

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Intracellular Imaging with a Graphene‐Based Fluorescent Probe

Cited by 260 publications

References 47 publications

Exceedingly biocompatible and thin-layered reduced graphene oxide nanosheets using an eco-friendly mushroom extract strategy

Exceedingly biocompatible and thin-layered reduced graphene oxide nanosheets using an eco-friendly mushroom extract strategy

Is Graphene a Promising Nano-Material for Promoting Surface Modification of Implants or Scaffold Materials in Bone Tissue Engineering?

Graphene-Based Nanomaterials for Theranostic Applications

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