Intracellular Glutamine Concentration Does Not Decrease in All Muscles during Sepsis

James, J. Howard; Hasselgren, Per-Olof; King, Jy-Kung; James, Laura E.; Fischer, Josef E.

doi:10.1006/jsre.1993.1085

Cited by 12 publications

(10 citation statements)

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“…Muscle glutamine concentration was approximately 40% lower in septic than in control patients, similar to previous reports in patients and experimental animals with muscle catabolism (12,18). Glycine and alanine levels were unchanged which is similar to a previous report in septic patients (12) but contrasts to reduced concentrations of these amino acids during severe sepsis in rats (18).…”

Section: Resultssupporting

confidence: 90%

“…Muscle concentration of phenylalanine was approximately twofold higher and that of 3-MH ‫ف‬ 60% higher in septic than in control patients. Similar differences have been reported previously in (12,18). The 3-MH/creatinine ratio in urine was 167 Ϯ 9.8 and 308 Ϯ54 nmol 3-MH/mg creatinine in control and septic patients, respectively (P Ͻ 0.05).…”

Section: Resultssupporting

confidence: 89%

“…Some of the muscle amino acid levels in the control and septic patients are shown in Fig 4. Changes in muscle concentrations of glutamine, glycine, alanine, 3-MH, tyrosine and phenylalanine were shown here because they have been found previously to be particularly affected by sepsis and other catabolic conditions (12,18). Muscle glutamine concentration was approximately 40% lower in septic than in control patients, similar to previous reports in patients and experimental animals with muscle catabolism (12,18).…”

Section: Resultssupporting

confidence: 88%

“…Plasma was deproteinized with 5% (wt/vol) sulfosalicylic acid containing 200 nmol/ml thienylalanine as internal standard and amino acid concentrations were measured in an automated amino acid analyzer (Beckman Amino Acid Analyzer; Beckman Instruments, Inc., Fullerton, CA). To measure tissue amino acids, the muscle biopsy specimens were homogenized in three volumes of 5% sulfosalicylic acid and after centrifugation at 5,000 g for 30 min, amino acids in the supernatant were measured by high performance liquid chromatography using a modification of the method of Graser et al (17) as described recently in a report from our laboratory (18).…”

Section: Methodsmentioning

confidence: 99%

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Sepsis is associated with increased mRNAs of the ubiquitin-proteasome proteolytic pathway in human skeletal muscle.

Tiao¹,

Hobler²,

Wang³

et al. 1997

J. Clin. Invest.

235

104

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Previous studies provided evidence that sepsis-induced muscle proteolysis in experimental animals is caused by increased ubiquitin-proteasome-dependent protein breakdown. It is not known if a similar mechanism accounts for muscle proteolysis in patients with sepsis. We determined mRNA levels for ubiquitin and the 20 S proteasome subunit HC3 by Northern blot analysis in muscle tissue from septic ( n ϭ 7) and non-septic ( n ϭ 11) patients. Plasma and muscle amino acid concentrations and concentrations in urine of 3-methylhistidine (3-MH), creatinine, and cortisol were measured at the time of surgery to assess the catabolic state of the patients. A three-to fourfold increase in mRNA levels for ubiquitin and HC3 was noted in muscle tissue from the septic patients concomitant with increased muscle levels of phenylalanine and 3-MH and reduced levels of glutamine. Total plasma amino acids were decreased by ‫ف‬ 30% in the septic patients. The 3-MH/creatinine ratio in urine was almost doubled in septic patients. The cortisol levels in urine were higher in septic than in control patients but this difference did not reach statistical significance. The results suggest that sepsis is associated with increased mRNAs of the ubiquitin-proteasome pathway in human skeletal muscle. ( J. Clin. Invest. 1997. 99:163-168.)

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Section: Resultssupporting

confidence: 90%

Section: Resultssupporting

confidence: 89%

Section: Resultssupporting

confidence: 88%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Sepsis is associated with increased mRNAs of the ubiquitin-proteasome proteolytic pathway in human skeletal muscle.

Tiao¹,

Hobler²,

Wang³

et al. 1997

J. Clin. Invest.

235

104

View full text Add to dashboard Cite

show abstract

“…Samples of medium (180 l) were deproteinized with 20 l of 30% (wt/vol) sulphosalicylic acid (SSA). Amino acids in muscle were analyzed using the HPLC method of Graser et al (38) with modifications as previously described (39). All supplies for amino acid analysis using the Beckman Model 6300 amino acid analyzer (amino acid calibration standards, ion-exchange column, buffers, and ninhydrin) were purchased from Beckman Instruments Inc. Sulfosalicylic acid (SSA) was from Fluka Chemical Corp. (Ronkonkoma, NY).…”

Section: Methodsmentioning

confidence: 99%

Linkage of aerobic glycolysis to sodium-potassium transport in rat skeletal muscle. Implications for increased muscle lactate production in sepsis.

James¹,

Fang²,

Schrantz³

et al. 1996

J. Clin. Invest.

147

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Although a linkage between aerobic glycolysis and sodiumpotassium transport has been demonstrated in diaphragm, vascular smooth muscle, and other cells, it is not known whether this linkage occurs in skeletal muscle generally. Metabolism of intact hind-leg muscles from young rats was studied in vitro under aerobic incubation conditions. When sodium influx into rat extensor digitorum longus (EDL) and soleus muscles was facilitated by the sodium ionophore monensin, muscle weight gain and production of lactate and alanine were markedly stimulated in a dose-dependent manner. Although lactate production rose in both muscles, it was more pronounced in EDL than in soleus. Monensininduced lactate production was inhibited by ouabain or by incubation in sodium-free medium. Preincubation in potassium-free medium followed by potassium re-addition also stimulated ouabain-inhibitable lactate release. Replacement of glucose in the incubation medium with pyruvate abolished monensin-induced lactate production but exacerbated monensin-induced weight gain. Muscles from septic or endotoxin-treated rats exhibited an increased rate of lactate production in vitro that was partially inhibited by ouabain. Increases muscle lactate production in sepsis may reflect linked increases in activity of the Na ϩ , K ϩ -ATPase, consumption of ATP and stimulation of aerobic glycolysis. ( J. Clin. Invest. 1996. 98:2388-2397.)

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Amino Acids | Glutamine

Watford¹

2021

Encyclopedia of Biological Chemistry III

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Intracellular Glutamine Concentration Does Not Decrease in All Muscles during Sepsis

Cited by 12 publications

References 0 publications

Sepsis is associated with increased mRNAs of the ubiquitin-proteasome proteolytic pathway in human skeletal muscle.

Sepsis is associated with increased mRNAs of the ubiquitin-proteasome proteolytic pathway in human skeletal muscle.

Linkage of aerobic glycolysis to sodium-potassium transport in rat skeletal muscle. Implications for increased muscle lactate production in sepsis.

Amino Acids | Glutamine

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