Intracellular Distribution of Fumarase, Aconitase, and Isocitric Dehydrogenase in Rabbit Cerebral Cortex

Shepherd, James A.; Kalnitsky, George

doi:10.1016/s0021-9258(18)65676-5

Cited by 43 publications

(2 citation statements)

References 14 publications

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“…F. Hogg, personal communication). Its observed distribution agrees with the results of other workers (22,29,42), who have found the greater part of it in the soluble fraction, and the remainder in the mitochondrial fraction. The recovery values listed in Table II (11).…”

Section: Fractionation Procedures and Enzyme Distributionssupporting

confidence: 92%

“…An original and surprising observation relates to the distribution of the NADP-linked isocitrate dehydrogenase. As first shown by Hogeboom and Schneider (29) on mouse liver and confirmed later on rabbit (42) and rat (22) liver, some 10-20% of this activity sediments with the mitochondrial fraction, the remainder being in the final supernatant. In Tetrahymena pyriformis, about one-half of the activity is soluble and most of the other half is associated with peroxisomes; the mitochondria contain small but significant amounts of this enzyme (M. Miller and J. F. Hogg, personal communication).…”

Section: Properties Of Particlessupporting

confidence: 61%

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The Large-Scale Separation of Peroxisomes, Mitochondria, and Lysosomes From the Livers of Rats Injected With Triton Wr-1339

Leighton

Poole

Beaufay

et al. 1968

The Journal of Cell Biology

1,103

310

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Improved, largely automated methods are described for the purification and analysis o peroxisomes, lysosomes, and mitochondria from the livers of rats injected with Triton WR-1339. With these new methods, it has become possible to obtain, in less than 6 hr and with reliable reproducibility, mitochondria practically free of contaminants, as well as the rarer cytoplasmic particles in amounts (about 100 mg of protein) and in a state of purity (95%) that make them suitable for detailed biochemical studies. The results obtained so far on these preparations have made more conclusive and precise previous estimates of the biochemical and morphological properties of the three groups of cytoplasmic particles. In addition, peroxisomes were found to contain essentially all the L-a-hydroxy acid oxidase of the liver, as well as a small, but significant fraction of its NADP-linked isocitrate dehydrogenase activity. Another small fraction of the latter enzyme is present in the mitochondria, the remainder being associated with the cell sap. The mitochondrial localization of the metabolically active cytoplasmic DNA could be verified. The relative content of the fractions in mitochondria, whole peroxisomes, peroxisome cores, lysosomes, and endoplasmic reticulum was estimated independently by direct measurements on electron micrographs, and by linear programming (based on the assumption that the particles are biochemically homogeneous) of the results of enzyme assays. The two types of estimates agreed very well, except for one fraction in which low cytochrome oxidase activity was associated with mitochondrial damage.

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Section: Fractionation Procedures and Enzyme Distributionssupporting

confidence: 92%

Section: Properties Of Particlessupporting

confidence: 61%