Intra-articular adipose-derived mesenchymal stem cells from rheumatoid arthritis patients maintain the function of chondrogenic differentiation

Abstract

Objectives. To evaluate the chondrogenic potential, phenotype and percentage of IA adipose-derived mesenchymal stem cells (ADSCs) from RA patients in comparison with OA patients. The effect of TNF treatment on ADSC differentiation was also examined. Methods. Adipose tissue was obtained from RA and OA patients. ADSCs were isolated and cultured until passage 4. After that period, the phenotype and percentage of these cells were analysed by flow cytometry. Passage 4 cells were cultured in chondrogenic medium with or without TNF. After 3 weeks of differentiation the expression of Sox9, aggrecan (Acan) and collagen 2a (Col2a) mRNA was assessed by RT-PCR and GAG deposition by alcian blue staining. Results. The phenotype and percentage of ADSCs were similar in both RA and OA. The results of alcian blue staining showed effective chondrogenesis in RA and OA ADSCs. TNF inhibited GAG deposition in both RA and OA samples similarly. Sox9, Acan and Col2a mRNA expression was significantly increased in chondrogenic-medium-treated cells (P < 0.05) and decreased after TNF exposure (P < 0.01). No statistically significant differences between RA and OA were observed. Conclusion. ADSCs from RA and OA patients are similar with regard to their phenotype, percentage in IA tissue and chondrogenic potential, which is reduced after exposure to TNF.

Yields and chondrogenic potential of primary synovial mesenchymal stem cells are comparable between rheumatoid arthritis and osteoarthritis patients

“…According to previous reports, the chondrogenic potential of passaged synovial MSCs in RA patients was similar to that in OA patients by Skalska et al [22] and Koizumi et al [23], contrarily, that in RA patients was inferior to that in OA patients by Jones et al [19]. Jones et al collected synovial tissues for biopsy and CRP in donors ranging between 0 and 15 mg/dl, contrarily the other studies, including ours, collected synovial tissues primarily during total knee arthroplasty and CRP in donors ranging only between 0 and 8 mg/dl.…”

Clinical immunology Comparison of phenotype, chondrogenic and osteogenic potential of rheumatoid mesenchymal stem cells derived from articular and subcutaneous adipose tissue - the role of adipocytokines

“…In our previous publications, we have reported that TNF is able to inhibit chondrogenesis of AAT-ASCs from RA and OA patients [21]. We have also shown that TNF has complex effect on AAT-ASCs osteogenesis: it up-regulates early osteogenesis markers mRNA expression and enhances calcium deposition, but decreases expression of mRNA coding for non-mineral bone components [22].…”

The importance and use of stem cells in dermatology

“…Tkankę tłuszczową białą i brunatną zasiedlają somatyczne komórki macierzyste pochodzenia mezenchymalnego [19]. Jest ona tanim, nieograniczonym rezerwuarem komórek macierzystych -z 300 ml tkanki tłuszczowej uzyskuje się 2-3 × 10 8 ADSCs, czyli od 100 do 1000 razy więcej niż mezenchymalnych komórek macierzystych ze szpiku kostnego [11,20].…”

“…To parakrynne wydzielanie cytokin tłumaczy ich duże stężenia u osób otyłych [22]. Mezenchymalne komórki zrębu tkanki tłuszczowej mogą się różni-cować również w osteoblasty, chondrocyty, miocyty oraz komórki neuronów, co daje ogromne możliwo-ści ich zastosowania w medycynie regeneracyjnej [11,19,20]. Obecnie wykorzystuje się je w dermatologii estetycznej do zabiegów odmładzania skóry, wygła-dzania zmarszczek, lipoatrofii twarzy oraz w seksuologii (poprawiają erekcję).…”

“…Obecnie wykorzystuje się je w dermatologii estetycznej do zabiegów odmładzania skóry, wygła-dzania zmarszczek, lipoatrofii twarzy oraz w seksuologii (poprawiają erekcję). Opisano ich zastosowanie w leczeniu przetok okołoodbytniczych w chorobie Leśniowskiego-Crohna, przeszczepach kostnych i cukrzycy typu 1 [19,22].…”