1990
DOI: 10.1055/s-2007-1014010 View full text |Buy / Rent full text
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Abstract: The patency-rate of venous bypasses depends - beside other important factors - upon the integrity of the transplanted vessel, especially on the condition of the tunica intima. Under this aspect we examined by light microscopy a total of over 1100 slices of human as well as animal vein-segments which had been stored in arterial heparinized blood or Euro-Collins-Solution at 4 degrees C, 20 degrees C and 37 degrees C up to 90 minutes. The results show that veins should be used immediately after harvesting. If int… Show more

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“…These results were in line with other published studies showing that vessel storage in PSS, HAB and other solutions led to endothelium damage, perhaps because of free radical injury, low pH, storage media composition or hostile environments [16][17][18][19][20]. Thatte and colleagues, demonstrated that human saphenous vein segments stored in DuraGraft (original name GALA: glutathione, ascorbic acid, L-arginine) showed enhanced endothelial structural integrity preservation under light microscopy compared to a range of solutions, including saline, HAB and Hank's balanced salt solution.…”
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rupbmjkragerfmgwileyiopcupepmcmbcthiemesagefrontiersapsiucrarxivemeralduhksmucshluniversity-of-gavle
“…These results were in line with other published studies showing that vessel storage in PSS, HAB and other solutions led to endothelium damage, perhaps because of free radical injury, low pH, storage media composition or hostile environments [16][17][18][19][20]. Thatte and colleagues, demonstrated that human saphenous vein segments stored in DuraGraft (original name GALA: glutathione, ascorbic acid, L-arginine) showed enhanced endothelial structural integrity preservation under light microscopy compared to a range of solutions, including saline, HAB and Hank's balanced salt solution.…”
supporting
“…This is known to contribute to a greater response to injury than undamaged grafts. Nevertheless, conclusions about endothelial lesions remain questionable since vein storage in a saline solution alters the endothelium [5]. Furthermore, eight veins (42%) showed important dissection of the media.…”
Section: Discussionmentioning
“…Although the ITA is harvested with a "no-touch" technique, saphenous vein and radial artery (RA) harvest frequently involve manual distension to resolve spasm and identify branches, followed by hypoxic storage. 5,6 These factors disrupt intimal integrity and increase the risk of early failure. 7,8 Although the ITA is the first-choice conduit, SV and RA grafts continue to be used for one or more grafts, even where bilateral ITA grafting is done.…”
mentioning