Intestinirhabdus alba gen. nov., sp. nov., a novel genus of the family Enterobacteriaceae, isolated from the gut of plastic-eating larvae of the Coleoptera insect Zophobas atratus

Abstract

A bacterial strain, BIT-B35T, was isolated from the gut of plastic-eating larvae of the Coleoptera insect Zophobas atratus. Its taxonomic position was determined by using a polyphasic approach. Cells were white-pigmented, Gram-stain-negative, motile short rods with terminal flagella. The 16S rRNA gene sequence (1411 bp) of strain BIT-B35T showed highest similarity (98.1%) to Escherichia fergusonii ATCC 35469T and Citrobacter koseri LMG 5519T. The results of phylogenetic analyses, based on the 16S rRNA gene, concatenated sequences of seven housekeeping genes (atpD, gyrB, infB, rpoB, pyrG, fusA and leuS) and genome sequences, placed strain BIT-B35T in a separate lineage among the family of Enterobacteriaceae . The major fatty acids were C16 : 0, C17 : 0 cyclo and C19 : 0 cyclo ω8c. The genomic DNA G+C content of strain BIT-B35T was 57.1 mol%. The chemotaxonomic data plus results of physiological and biochemical tests also distinguished strain BIT-B35T from members of other genera within the family Enterobacteriaceae . Therefore, strain BIT-B35T is considered to represent a novel species of a novel genus within the family Enterobacteriaceae , for which the name Intestinirhabdus alba gen. nov., sp. nov. is proposed. The type strain is BIT-B35T (=CGMCC 1.17042T=KCTC 72448T).

Midgut bacterial diversity of a leaf-mining beetle, Dactylispa xanthospila (Gestro) (Coleoptera: Chrysomelidae: Cassidinae)

“… Enterobacteriaceae is considered to be the most prevalent symbiotic microorganism in dipteran insects ( Kuzina et al 2001 ). Some Enterobacteriaceae play an important role in plastic biodegradation ( Xu et al 2020 ). Many Enterobacteriaceae can fix nitrogen and produce necessary nutrients for the hosts ( Behar et al 2005 ).…”

Reclassification of Shigella species as later heterotypic synonyms of Escherichia coli in the Genome Taxonomy Database

“…The 23,686 genomes classified as Escherichia in GTDB R06-RS202 or Escherichia/Shigella according to the NCBI Taxonomy (Schoch et al 2020; downloaded September 23, 2020) were considered in this study. Two notable exceptions are inclusion of the E. ruysiae type strain genome GCF_902498915.1 which was released after GTDB R06-RS202 and filtering of the genome GCF_009711095.1 which is classified as ‘ E. alba’ at NCBI but recognized as belonging to the genus Intestinirhabdus (Xu et al, 2020). The E. ruysiae type strain genome (GCF_902498915.1) was compared to GTDB species representatives in Escherichia and found to be highly similar to the representative of Escherichia sp000208585 (GCF_000208585.1) with an ANI of 99.8% and AF of 0.97.…”

Tenebrionibacter intestinalis gen. nov., sp. nov., a member of a novel genus of the family Enterobacteriaceae, isolated from the gut of the plastic-eating mealworm Tenebrio molitor L.

“…However, in 2016, according to a comprehensive genome-based taxonomic analysis, the order ‘ Enterobacteriales ’ was renamed as the order Enterobacterales and divided into seven families ( Enterobacteriaceae , Erwiniaceae , Hafniaceae , Morganellaceae , Pectobacteriaceae , Budviciaceae and Yersiniaceae ) [1, 2]. To date, the newly described family Enterobacteriaceae comprises 33 validly published genera and more than 130 validly published species according to the List of Prokaryotic Names with Standing in Nomenclature (www.bacterio.net) [3]. Species within the family Enterobacteriaceae are widely distributed in nature, and a large number of them were isolated from the guts of human and animals [4–9].…”