Intestinal Toxemia Botulism in Two Young People, Caused by Clostridium butyricum Type E

Fenicia, Lucia; Franciosa, Giovanna; Pourshaban, M.; Aureli, Paolo

doi:10.1086/313497

Cited by 93 publications

(64 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…6,8 Se planteó también la hipótesis de que la producción de toxina en el ciego podría paralizar inicialmente la válvula ileocecal y permitir el reflujo de la toxina en el intestino delgado, donde la absorción es más eficiente. 12 El diagnóstico de botulismo infantil se realiza evidenciando el Clostridium botulinum y su toxina en heces; en suero, solo es posible detectar la toxina. [4][5][6]10 El electromiograma ayuda y apoya el diagnóstico y no se descarta en caso de normalidad.…”

Section: Discussionunclassified

Invaginación intestinal en un lactante con diagnóstico de botulismo. Reporte de un caso

et al. 2015

View full text Add to dashboard Cite

Arch Argent Pediatr 2015;113(5):e286-e289 / e286Presentación de casos clínicos RESUMEN El botulismo es un importante problema de salud pública en Argentina. Es una enfermedad potencialmente letal y de difícil diagnóstico. Existen casos de presentación infrecuente de dicha enfermedad, como el abdomen agudo. Exponemos el caso de un niño de 4 meses, que consultó por constipación de 3 días de evolución, asociada a decaimiento y regular actitud alimentaria de 12 horas de evolución. Presentaba tono muscular conservado, sin alteraciones en la succión ni deglución, según la referencia materna. Se constató sensorio alternante y abdomen agudo, por lo que ingresó a quirófano con sospecha de invaginación intestinal, la cual fue confirmada mediante desinvaginación neumática. Durante la internación, el paciente evolucionó desfavorablemente y presentó llanto débil, hipotonía progresiva e insuficiencia respiratoria, por lo que requirió cuidados intensivos. Se aisló Clostridium botulinum en la muestra de materia fecal y toxina botulínica tipo A en el suero. Recibió toxina antibotulínica equina como tratamiento, con recuperación total a los 25 días de haber ingresado. Palabras clave: botulismo, hipotonía muscular, Clostridium botulinum, invaginación.

show abstract

Section: Discussionunclassified

Invaginación intestinal en un lactante con diagnóstico de botulismo. Reporte de un caso

et al. 2015

View full text Add to dashboard Cite

show abstract

“…It results from colonization of the intestinal tract by toxin-producing clostridia (40,64,143,145). People with altered intestinal flora due to abdominal surgery (76,111), prolonged antimicrobial treatment, or gastrointestinal wounds and abscesses are particularly vulnerable (40).…”

Section: Infectious Botulism In Adultsmentioning

confidence: 99%

Laboratory Diagnostics of Botulism

2006

View full text Add to dashboard Cite

SUMMARY Botulism is a potentially lethal paralytic disease caused by botulinum neurotoxin. Human pathogenic neurotoxins of types A, B, E, and F are produced by a diverse group of anaerobic spore-forming bacteria, including Clostridium botulinum groups I and II, Clostridium butyricum, and Clostridium baratii. The routine laboratory diagnostics of botulism is based on the detection of botulinum neurotoxin in the patient. Detection of toxin-producing clostridia in the patient and/or the vehicle confirms the diagnosis. The neurotoxin detection is based on the mouse lethality assay. Sensitive and rapid in vitro assays have been developed, but they have not yet been appropriately validated on clinical and food matrices. Culture methods for C. botulinum are poorly developed, and efficient isolation and identification tools are lacking. Molecular techniques targeted to the neurotoxin genes are ideal for the detection and identification of C. botulinum, but they do not detect biologically active neurotoxin and should not be used alone. Apart from rapid diagnosis, the laboratory diagnostics of botulism should aim at increasing our understanding of the epidemiology and prevention of the disease. Therefore, the toxin-producing organisms should be routinely isolated from the patient and the vehicle. The physiological group and genetic traits of the isolates should be determined.

show abstract

“…The remaining C. butyricum type E strains had been isolated in Italy from clinical samples from patients suffering from botulism (1,2,10).…”

Section: Methodsmentioning

confidence: 99%

Identification of Type A, B, E, and F Botulinum Neurotoxin Genes and of Botulinum Neurotoxigenic Clostridia by Denaturing High-Performance Liquid Chromatography

Franciosa

Pourshaban

Luca

et al. 2004

Appl Environ Microbiol

View full text Add to dashboard Cite

Denaturing high-performance liquid chromatography (DHPLC) is a recently developed technique for rapid screening of nucleotide polymorphisms in PCR products. We used this technique for the identification of type A, B, E, and F botulinum neurotoxin genes. PCR products amplified from a conserved region of the type A, B, E, and F botulinum toxin genes from Clostridium botulinum, neurotoxigenic C. butyricum type E, and C. baratii type F strains were subjected to both DHPLC analysis and sequencing. Unique DHPLC peak profiles were obtained with each different type of botulinum toxin gene fragment, consistent with nucleotide differences observed in the related sequences. We then evaluated the ability of this technique to identify botulinal neurotoxigenic organisms at the genus and species level. A specific short region of the 16S rRNA gene which contains genus-specific and in some cases species-specific heterogeneity was amplified from botulinum neurotoxigenic clostridia and from different food-borne pathogens and subjected to DHPLC analysis. Different peak profiles were obtained for each genus and species, demonstrating that the technique could be a reliable alternative to sequencing for the rapid identification of food-borne pathogens, specifically of botulinal neurotoxigenic clostridia most frequently implicated in human botulism.

show abstract

Intestinal Toxemia Botulism in Two Young People, Caused by Clostridium butyricum Type E

Cited by 93 publications

References 27 publications

Invaginación intestinal en un lactante con diagnóstico de botulismo. Reporte de un caso

Invaginación intestinal en un lactante con diagnóstico de botulismo. Reporte de un caso

Laboratory Diagnostics of Botulism

Identification of Type A, B, E, and F Botulinum Neurotoxin Genes and of Botulinum Neurotoxigenic Clostridia by Denaturing High-Performance Liquid Chromatography

Contact Info

Product

Resources

About