Intestinal remodelling in mink fed with reduced protein content

Chen, Pengmin; Zhao, Jingbo; Nielsen, V. H.; Clausen, T.

doi:10.1016/j.jbiomech.2008.11.033

Cited by 11 publications

(9 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is likely due to the reduced smooth muscle mass. Indeed, we found in the present study that the smooth muscle layer thickness of duodenum was decreased in rabbits on low‐fiber diet concomitant to the fasting and low‐protein animal models . In addition, the maximum contraction amplitudes were positively associated with muscle layer thickness with the circumferential muscle layer as the main determining factor (Figure , Table ).…”

Section: Discussionsupporting

confidence: 55%

See 1 more Smart Citation

Stress–strain analysis of duodenal contractility in response to flow and ramp distension in rabbits fed low‐fiber diet

Liu

Zhao

Liao

et al. 2018

Neurogastroenterology Motil

Self Cite

View full text Add to dashboard Cite

Background Previously we demonstrated that low‐fiber diet in rabbits affects the passive mechanomorphological properties in the small intestine, resulting in reduced intestinal wall thickness and collagen content, as well as intestinal wall softening. The aim of the present study was to evaluate the contractility in rabbits on long‐term low‐fiber diet and specifically to compare the contraction threshold, the frequency, and the amplitude of flow‐induced and distension‐induced contractions in the duodenum between rabbits on normal diet and on long‐term low‐fiber diet. Methods Ten rabbits were fed a low‐fiber diet for 5 months (Intervention group), and five rabbits were fed normal diet (Control group). The duodenal segments were used for determination of mechanical parameters for analyses of contractility. The duodenal experiments were carried out in organ baths containing physiological Krebs solution. Pressure and diameter changes induced by contractions in response to flow and ramp distension were measured. The frequencies and amplitude of contractions were analyzed. Distension‐induced contraction thresholds and maximum contraction amplitude of flow‐induced contractions were calculated in terms of mechanical stress and strain. Multiple linear regression analyses were applied to study dependencies between contractility parameters and wall thickness, wall area, and muscle layer thickness. Key results During distension, the pressure, stress, and strain thresholds for induction of phasic contraction were biggest in the Intervention Group (P < 0.05). In addition, the contraction frequencies during flow‐induced contraction were highest in the Intervention Group (P < 0.05), whereas the maximum contraction amplitudes in terms of pressure, diameter, stress, and strain were lowest in the Intervention Group (P < 0.05). The contraction thresholds and contraction frequencies were negatively associated with the wall thickness, wall area, and muscle layer thickness, whereas maximum contraction amplitudes were positively associated with the wall thickness, wall area, and muscle layer thickness. Conclusions and inferences Duodenal contractility in rabbits fed with long‐term low‐fiber diet exhibited low contraction amplitudes and high contraction thresholds and frequencies. The changes were associated with the low‐fiber diet‐induced histomorphological remodeling. Studies on detailed structural and functional diet‐induced changes in smooth muscle and intestinal nerves are needed for better understanding the remodeling mechanisms.

show abstract

Section: Discussionsupporting

confidence: 55%

“…A low‐fiber diet is a diet containing less fiber or totally lack fiber. Therefore, rabbits fed with low‐fiber diet are exposed to malnutrition which is somewhat similar to fasting models and reduced protein diet models . It has been reported that fasting for 12 hours and 48 hours reduced intestinal motility.…”

Section: Discussionmentioning

confidence: 99%

Stress–strain analysis of duodenal contractility in response to flow and ramp distension in rabbits fed low‐fiber diet

Liu

Zhao

Liao

et al. 2018

Neurogastroenterology Motil

Self Cite

View full text Add to dashboard Cite

show abstract

“…Rabbits fed with low‐residue diet are in a malnutrition situation . Therefore, the present rabbit model is somewhat similar to rat fasting model and reduced protein diet in mink . In this study, we demonstrated that the rabbits fed with low‐residue diet had reduced wall thickness and area which was primarily due to mucosal thinning.…”

Section: Discussionmentioning

confidence: 53%

“…Refeeding quickly restored normal conditions . Later, Chen and coworkers demonstrated that feeding a low‐protein diet reduced bodyweight, intestinal wet weight, wall thickness, and stiffness of jejunum and ileum . The abovementioned studies demonstrated that lack of nutrition and malnutrition affects the intestinal morphometry and biomechanical properties.…”

Section: Introductionmentioning

confidence: 98%

Low‐residue diet fed to rabbits induces histomorphological and biomechanical remodeling of small intestine

Liu

Zhao

Liao

et al. 2016

Neurogastroenterology Motil

View full text Add to dashboard Cite

show abstract

“…The higher k a obtained in PEU animals compared with WN animals may be a consequence of changes in the absorptive membrane, like altered mucosal protein concentrations (absorptive and secretory carriers, and also metabolic enzymes), reduced villus heights and crypt depths, and also disrupted intestinal membrane integrity or damage in tight junctions [7,50,52–55]. In this nutritional status, the number of protein carriers would be decreased, and the repercussion of inhibitors of influx or efflux transport would have a minor effect.…”

Section: Discussionmentioning

confidence: 99%

In Situ Study of the Effect of Naringin, Talinolol and Protein-Energy Undernutrition on Intestinal Absorption of Saquinavir in Rats

Catalán-Latorre

Nácher

Merino

et al. 2011

Basic &Amp; Clinical Pharmacology &Amp; Toxicology

View full text Add to dashboard Cite

To study the potential interactions of naringin (NAR), talinolol (TAL) and protein-energy undernutrition (PEU) in the absorption process of saquinavir (SQV), perfusion experiments were performed in the small intestine of rats at different SQV concentrations. The results obtained demonstrated that SQV intestinal absorption was described by simultaneous passive diffusion (k dif = 3.44 hr) and saturable absorption (V ma = 127.31 lM ⁄ hr; K ma = 10.50 lM) together with a capacity-limited efflux (V ms = 270.53 lM ⁄ hr; K ms = 23.44 lM). The competitive inhibition constants of NAR on the SQV input and efflux processes were [IC50] a = 3.98 lM and [IC50] s = 5.00 lM, respectively. NAR significantly decreased (23-29%; p < 0.05) or kept unaltered the absorption rate constant (k a ) of SQV in function of the concentration of both compounds administered. Finally, SQV k a significantly increased in PEU status (around 1.8 times) when the drug was perfused either in the presence (p < 0.05) or in the absence (p < 0.01) of NAR. The variations of SQV k a when the antiretroviral drug is co-administered with NAR and ⁄ or TAL reinforce their interaction in the absorptive process. Malnutrition may result in altered SQV absorption, and further studies are strongly recommended to analyse the impact of this finding on the pharmacokinetic drug profile.

show abstract

Intestinal remodelling in mink fed with reduced protein content

Cited by 11 publications

References 20 publications

Stress–strain analysis of duodenal contractility in response to flow and ramp distension in rabbits fed low‐fiber diet

Stress–strain analysis of duodenal contractility in response to flow and ramp distension in rabbits fed low‐fiber diet

Low‐residue diet fed to rabbits induces histomorphological and biomechanical remodeling of small intestine

In Situ Study of the Effect of Naringin, Talinolol and Protein-Energy Undernutrition on Intestinal Absorption of Saquinavir in Rats

Contact Info

Product

Resources

About