Intervention Effects of Nedaplatin and Cisplatin on Proliferation and Apoptosis of Human Tumour Cells in Vitro

Su, Xiangyu; Yin, Haitao; Li, Suyi; Huang, Xin-En; Tan, Hua-Yang; Dai, Hongyu; Shi, Fang

doi:10.7314/apjcp.2012.13.9.4531

Cited by 12 publications

(13 citation statements)

References 13 publications

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“…Eight clinically relevant chemotherapeutic agents were tested on their ability to cross the BBB and their cytotoxic effects on glioma cells. The properties of these agents are listed in Supplementary Table 13738394041424344. Among them, only Temozolomide (TMZ) is lipophilic and permeable to BBB, which is used for the treatment of glioblastoma multiforme in clinics.…”

Section: Resultsmentioning

confidence: 99%

“…Given its solubility, TMZ was dissolved in DMSO, and the other drugs were dissolved in PBS. All eight drugs were prepared at a concentration twice that of their respective IC 50 values3738394041424344. Three groups of experiments were performed: control group (no barrier), BMECs mono-cultured group and BBB group (BMECs co-cultured with astrocytes).…”

Section: Methodsmentioning

confidence: 99%

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A dynamic in vivo-like organotypic blood-brain barrier model to probe metastatic brain tumors

et al. 2016

Sci Rep

167

152

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The blood-brain barrier (BBB) restricts the uptake of many neuro-therapeutic molecules, presenting a formidable hurdle to drug development in brain diseases. We proposed a new and dynamic in vivo-like three-dimensional microfluidic system that replicates the key structural, functional and mechanical properties of the blood-brain barrier in vivo. Multiple factors in this system work synergistically to accentuate BBB-specific attributes–permitting the analysis of complex organ-level responses in both normal and pathological microenvironments in brain tumors. The complex BBB microenvironment is reproduced in this system via physical cell-cell interaction, vascular mechanical cues and cell migration. This model possesses the unique capability to examine brain metastasis of human lung, breast and melanoma cells and their therapeutic responses to chemotherapy. The results suggest that the interactions between cancer cells and astrocytes in BBB microenvironment might affect the ability of malignant brain tumors to traverse between brain and vascular compartments. Furthermore, quantification of spatially resolved barrier functions exists within a single assay, providing a versatile and valuable platform for pharmaceutical development, drug testing and neuroscientific research.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

A dynamic in vivo-like organotypic blood-brain barrier model to probe metastatic brain tumors

et al. 2016

Sci Rep

167

152

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“…Cisplatin is a broad-spectrum anticarcinogen, which is also used in combination with hyperthermia (Issels, 2008). The tumor cell inhibition of high doses of cisplatin (80 and 120 μg/ml) at 24 h in vitro is above 80% (Hartmann et al, 2014;Su et al, 2012). Anucleated erythrocytes are little damaged by cisplatin in theory because the key molecular target of cisplatin is eukaryotic DNA (Issels, 2008).…”

Section: Discussionmentioning

confidence: 99%

Cisplatin combined with hyperthermia kills HepG2 cells in intraoperative blood salvage but preserves the function of erythrocytes

Yang

Tang

Liu

et al. 2015

J. Zhejiang Univ. Sci. B

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Abstract:The safe use of intraoperative blood salvage (IBS) in cancer surgery remains controversial. Here, we investigated the killing effect of cisplatin combined with hyperthermia on human hepatocarcinoma (HepG2) cells and erythrocytes from IBS in vitro. HepG2 cells were mixed with concentrated erythrocytes and pretreated with cisplatin (50, 100, and 200 μg/ml) alone at 37 °C for 60 min and cisplatin (25, 50, 100, and 200 μg/ml) combined with hyperthermia at 42 °C for 60 min. After pretreatment, the cell viability, colony formation and DNA metabolism in HepG2 and the Na + -K + -ATPase activity, 2,3-diphosphoglycerate (2,3-DPG) concentration, free hemoglobin (Hb) level, osmotic fragility, membrane phosphatidylserine externalization, and blood gas variables in erythrocytes were determined. Pretreatment with cisplatin (50, 100, and 200 μg/ml) combined with hyperthermia (42 °C) for 60 min significantly decreased HepG2 cell viability, and completely inhibited colony formation and DNA metabolism when the HepG2 cell concentration was 5×10 4 ml −1 in the erythrocyte (P<0.01). Erythrocytic Na + -K + -ATPase activity, 2,3-DPG level, phosphatidylserine externalization, and extra-erythrocytic free Hb were significantly altered by hyperthermia plus high concentrations of cisplatin (100 and 200 μg/ml) (P<0.05), but not by hyperthermia plus 50 μg/ml cisplatin (P>0.05). In conclusion, pretreatment with cisplatin (50 μg/ml) combined with hyperthermia (42 °C) for 60 min effectively eliminated HepG2 cells from IBS but did not significantly affect erythrocytes in vitro.

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“…Then, the cells were treated with 10 µl of 64 mg/l NDP in the opti-MEM (Corning) medium. The NDP concentration was compared with the results of Su et al (13). The cells incubated in the opti-MEM medium were set up with the control group and incubated for 48 h at 37˚C.…”

Section: Construction Of Cytotoxicity Cell Model and Assay Eca109mentioning

confidence: 99%

MicroRNA and target mRNA selection through invasion and cytotoxicity cell modeling and bioinformatics approaches in esophageal squamous cell carcinoma

Song

et al. 2017

Oncology Reports

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This study analyzed microRNA (miRNA) and mRNA expression profiles and investigated the biological characteristics of ESCC by using invasion and cytotoxicity cell models. miRNA profiles were evaluated through miRNA microarray. Transwell chamber and nedaplatin (NDP) were used to construct invasion and cytotoxicity cell models. Invasion Transwell and cytotoxicity assays were performed to examine the invasiveness and proliferation in the cell models. Functional miRNAs were selected from dysregulated miRNAs through qRT-PCR. Biometric Research Program (BRB)-array tools, Cytoscape plugins, and DAVID were utilized to find potential mRNAs targeted by these two miRNAs between ESCC and paired normal adjacent tissues. Our microarray obtained 11 dysregulated miRNAs expressed in three paired ESCC samples from Kazakhs (ethnicity in Northwestern China). qRT-PCR demonstrated the miRNA expression in the invasion and cytotoxicity cell models. miR‑652-5p and miR‑21‑5p exhibited a consistent expression level in the microarray and cell models. Bioinformatics revealed that the potential targets of PLD1, MSH2, STC1, and DSG1 might be involved in ESCC invasion and proliferation. Cell models with bioinformatics approaches may help distinguish functional genes. miR‑652-5p, miR‑21‑5p, and their potential target genes may participate in ESCC development and metastasis.

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Intervention Effects of Nedaplatin and Cisplatin on Proliferation and Apoptosis of Human Tumour Cells in Vitro

Cited by 12 publications

References 13 publications

A dynamic in vivo-like organotypic blood-brain barrier model to probe metastatic brain tumors

A dynamic in vivo-like organotypic blood-brain barrier model to probe metastatic brain tumors

Cisplatin combined with hyperthermia kills HepG2 cells in intraoperative blood salvage but preserves the function of erythrocytes

MicroRNA and target mRNA selection through invasion and cytotoxicity cell modeling and bioinformatics approaches in esophageal squamous cell carcinoma

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