Internalization of novel non-viral vector TAT-streptavidin into human cells

Rinne, Johanna; Albarran, Brian; Jylhävä, Juulia; Ihalainen, Teemu O.; Kankaanpää, Pasi; Hytönen, Vesa P.; Stayton, Patrick S.; Kulomaa, Markku S.; Vihinen‐Ranta, Maija

doi:10.1186/1472-6750-7-1

Cited by 108 publications

(59 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The effects of cytochalasin D and of amiloride indicate that macropinocytosis is predominantly involved in the cell entry of MCa b -Strep-Cy5. This observation is consistent with many other reports that indicate a role of macropinocytosis in cell entry of other CPPs (14,29). However, the lack of effect of methyl-␤-cyclodextrin appears to indicate that endocytosis of MCa b -Strep-Cy5 is not dependent on lipid rafts or at least on cholesterol availability.…”

Section: The Molar Ratio Mca B /Strep-cy3 Does Notsupporting

confidence: 82%

Direct Peptide Interaction with Surface Glycosaminoglycans Contributes to the Cell Penetration of Maurocalcine

Ram

Aroui

Jaumain

et al. 2008

Journal of Biological Chemistry

View full text Add to dashboard Cite

Section: The Molar Ratio Mca B /Strep-cy3 Does Notsupporting

confidence: 82%

Direct Peptide Interaction with Surface Glycosaminoglycans Contributes to the Cell Penetration of Maurocalcine

Ram

Aroui

Jaumain

et al. 2008

Journal of Biological Chemistry

View full text Add to dashboard Cite

“…Our data support the conclusions of earlier studies involving a range of CPP-conjugated nanoparticles, such as TAT quantum dots and gold particles, 7d,9 and CPP-conjugated macromolecules such as TAT– streptavidin. 18 In all cases, internalization relied on energy-dependent endocytosis mechanisms.…”

Section: Resultsmentioning

confidence: 99%

Development of viral nanoparticles for efficient intracellular delivery

Chen

Yildiz

et al. 2012

Nanoscale

View full text Add to dashboard Cite

Viral nanoparticles (VNPs) based on plant viruses such as Cowpea mosaic virus (CPMV) can be used for a broad range of biomedical applications because they present a robust scaffold that allows functionalization by chemical conjugation and genetic modification, thereby offering an efficient drug delivery platform that can target specific cells and tissues. VNPs such as CPMV show natural affinity to cells; however, cellular uptake is inefficient. Here we show that chemical modification of the CPMV surface with a highly reactive, specific and UV-traceable hydrazone linker allows bioconjugation of polyarginine (R5) cell penetrating peptides (CPPs), which can overcome these limitations. The resulting CPMV–R5 particles were taken up into a human cervical cancer cell line (HeLa) more efficiently than native particles. Uptake efficiency was dependent on the density of R5 peptides on the surface of the VNP; particles displaying 40 R5 peptides per CPMV (denoted as CPMV–R5H) interact strongly with the plasma membrane and are taken up into the cells via an energy-dependent mechanism while particles displaying 10 R5 peptides per CPMV (CPMV–R5L) are only slowly taken up. The fate of CPMV–R5 versus native CPMV particles within cells was evaluated in a co-localization time course study. It was indicated that the intracellular localization of CPMV–R5 and CPMV differs; CPMV remains trapped in Lamp-1 positive endolysosomes over long time frames; in contrast, 30–50% of the CPMV–R5 particles transitioned from the endosome into other cellular vesicles or compartments. Our data provide the groundwork for the development of efficient drug delivery formulations based on CPMV–R5.

show abstract

“…(d) MβCD and nystatin treatment: cells were pretreated with 10 mM M β CD or 25 μg mL −1 nystatin in PBS buffer for 35 min at 37 °C to deplete membrane cholesterol44 before incubation with CCNPs. (e) Macropinocytosis inhibition: Cells were pre-incubated for 35 min with 5 μM cyto-D45 and 2 μM LATB,46 respectively, followed by incubation with CCNPs. The drugs were maintained during the incubation with CCNPs.…”

Section: Methodsmentioning

confidence: 99%

Unveiling the mechanism of uptake and sub-cellular distribution of cerium oxide nanoparticles

et al. 2010

View full text Add to dashboard Cite

Cerium oxide nanoparticles (CNPs) have been recently studied for their potent superoxide scavenging properties in both cell and animal model systems. Data from these model systems have shown that exposure of cells to CNPs results in the protection against reactive oxygen species (ROS). Despite these exciting findings, very little is known regarding the uptake or subcellular distribution of these nanomaterials inside cells. In this study we utilized fluorophore (carboxyfluorescein) conjugated cerium oxide NPs (CCNPs) to study the mechanism of uptake and to elucidate the subcellular localization of CNPs using a keratinocyte model system. We observed rapid uptake (within 3 h) of CCNPs that was governed by energy-dependent, clathrinmediated and caveolae-mediated endocytic pathways. We found CCNPs co-localized with mitochondria, lysosomes and endoplasmic reticulum as well as being abundant in the cytoplasm and the nucleus. Given the radical scavenging properties of cerium oxide and the widespread cellular disposition we observed, CNPs likely act as cellular antioxidants in multiple compartments of the cell imparting protection against a variety of oxidant injuries.

show abstract

Internalization of novel non-viral vector TAT-streptavidin into human cells

Cited by 108 publications

References 50 publications

Direct Peptide Interaction with Surface Glycosaminoglycans Contributes to the Cell Penetration of Maurocalcine

Direct Peptide Interaction with Surface Glycosaminoglycans Contributes to the Cell Penetration of Maurocalcine

Development of viral nanoparticles for efficient intracellular delivery

Unveiling the mechanism of uptake and sub-cellular distribution of cerium oxide nanoparticles

Contact Info

Product

Resources

About