Interleukin‐8 Gene Promoter Polymorphism (rs4073) May Contribute to Chronic Periodontitis

Abstract

Background The proinflammatory chemokine interleukin-8 (IL8) is important in the regulation of the inflammatory response. Single nucleotide polymorphism (SNP) rs4073 have shown that the A allele up regulates the IL8 levels after stimulation with lipopolysaccharides. We investigated the association of the SNP rs4073 with chronic periodontitis. Methods Genotyping was performed by standard polymerase chain reaction-restriction fragment length polymorphism assay in 289 genomic DNA samples of healthy control and chronic periodontitis patients; analyses were adjusted by the multivariate logistic regression modeling. Real-time PCR performance was used to detect the levels of the IL8 mRNA. Results Analysis points to a statistical significant association of chronic periodontitis with the heterozygous TA genotype (p = 0.001); the results showed an increase in the frequency of the A allele in the diseased group (36% in the control × 48% in the periodontitis group). The higher levels of the IL8 mRNA were found in the periodontitis group, mainly in individuals who presented the TA genotype (p=0.03). Conclusion The SNP rs4073 is associated with chronic periodontitis, in non-smoker Brazilian subjects, since the frequency of A allele is higher in the disease than control group and the TA genotype was associated with increased levels of IL8 mRNA transcripts.

Genetic analysis of the IL8 gene polymorphism (rs4073) in generalized aggressive periodontitis

“…In another study of the association between the IL8 rs4073 and generalized chronic periodontitis, 36 the TA genotype was associated with increased risk, at a frequency of 52.8% in controls versus 74.6% in chronic periodontitis patients. In the current study, the authors found a frequency of 65.8% of the TA genotype in the generalized AgP patients.…”

“…34–36 A recent study with the same IL8 SNP (rs4073), also in a Brazilian population, 34 reported lack of association with chronic periodontitis. In a subsequent study, 35 these same authors investigated the SNP (rs4073) as part of combined haplotypes (−845TC, −738TA and −353AT), and reported that nonsmokers individuals carrying the TAT/CTA genotype were six times more likely to develop chronic periodontitis.…”

<em>TIMP2</em> gene polymorphism as a potential tool to infer Brazilian population origin

“…The ethnic distribution of residents in São Paulo is 71.8% Caucasian, 4.2% African descendants, 22.6% Mulattos (children of intermarriages between Caucasians and African descendants), and 1.5% Japanese and Amerindian. 17 According to Callegari-Jacques and Salzano, [19][20][21] However, the Japanese population has rather limited genetic diversity, according to data from the Japanese Millennium Genome Project. 22 Population-specific SNPs may have important applications in anthropologic studies, such as studies of population genetic origins.…”

A Novel Single Nucleotide Polymorphism of the Interleukin-8 Promoter: Its Transcriptional Regulation and Analysis of the Mutation in Periodontal Disease in the Japanese Population

“…. This SNP (-251T/A) was associated with chronic periodontitis and other diseases like bronchiolitis, oral squamous cell carcinoma, and gastric cancer 24,[30][31][32] . Our findings showed that the transcriptional activity of the IL8 gene mediating -845T was 9-fold higher than that mediating -845C in SCCTM cells (Fig.…”

“…Interestingly, numerous SNPs have been found in the IL8 gene, some of which are known to be associated with susceptibility to diseases such as asthma and oral lichen planus 22,23) . A recent study showed that the SNP at position -251 of the IL8 promoter was associated with chronic periodontitis in Brazilian subjects 24) . Increased expression of the IL8 gene has also been observed in affected periodontal tissues 25) .…”