Interleukin-6 Concentration in the Transgenic Pig's Liver Preserved for 24 Hours in Biolasol Solution

Budziński, G.; Suszka-Świtek, Aleksandra; Roman, P.; Caban, A.; Oczkowicz, G.; Wiaderkiewicz, Anna; Maly, Alex; Wiaderkiewicz, Ryszard; Smorąg, Z.; Ryszka, Florian; Cierpka, L.

doi:10.1016/j.transproceed.2014.09.047

Cited by 6 publications

(6 citation statements)

References 10 publications

(3 reference statements)

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“…Sodium bicarbonate acts as a buffer that maintains the acid–base balance of the transplanted organ [ 14 , 15 ]. Budziński et al [ 16 ] analysed the effectiveness of Biolasol in an animal model of transgenic pigs with the transferred human gene: α1,2-fucosyltransferase (group I and II), α-galactosidase (III), combined α1,2-fucosyltransferase/α-galactosidase transgene (IV), and livers without modification (V). The isolated livers were perfused and stored in Biolasol for 24 h. In the collected perfundates and homogenates, they analysed IL-6 (interleukin-6) concentration.…”

Section: Discussionmentioning

confidence: 99%

Biochemical Studies in Perfundates and Homogenates of Isolated Porcine Kidneys after Flushing with Zinc or Zinc–Prolactin Modified Preservation Solution Using a Static Cold Storage Technique

2021

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Zinc is an effective anti-inflammatory and antioxidant trace element. The aim of this study was to analyse the protective effect of zinc and zinc–prolactin systems as additives of preservation solutions in the prevention of nephron damage caused during ischemia. The study used a model for storing isolated porcine kidneys in Biolasol®. The solution was modified with the addition of Zn at a dose of 1 µg/L and Zn: 1 µg/L with prolactin (PRL): 0.1 µg/L. After 2 h and 48 h of storage, the levels of alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, sodium, potassium, creatinine and total protein were determined. Zinc added to the Biolasol® composition at a dose of 1 µg/L showed minor effectiveness in the protection of nephrons. In turn, Zn2+ added to Biolasol + PRL (PRL: 0.1 µg/L) acted as a prolactin inhibitor. We do not recommend the addition of Zn(II) (1 µg/L) and Zn(II) (1 µg/L) + PRL (0.1 µg/L) to the Biolasol solution.

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Section: Discussionmentioning

confidence: 99%

Biochemical Studies in Perfundates and Homogenates of Isolated Porcine Kidneys after Flushing with Zinc or Zinc–Prolactin Modified Preservation Solution Using a Static Cold Storage Technique

2021

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“…Interestingly, the Biolasol solution was developed to offer better homeostasis and restoring of proper organ functioning after transplantation than previously used traditional solutions 7‐9 . According to its manufacturer, it is an extracellular fluid with a sodium concentration of 105 mmol/L and potassium concentration of 10 mmol/L.…”

Section: Discussionmentioning

confidence: 99%

“…It was supposed to offer better homeostasis maintenance during organ preservation 7 . Following the experimental investigations based on animal models, 8,9 an attempt was made to implement Biolasol into clinical practice in the setting of kidney transplantation. The aim of the present single‐center study was to summarize the initial clinical experience regarding the early kidney graft function and intrarenal Doppler resistive indices of kidneys preserved using Biolasol and those preserved using other solutions.…”

Section: Introductionmentioning

confidence: 99%

Early graft function and intrarenal resistant index after kidney transplantation using Biolasol—A new solid organ preservation fluid

et al. 2020

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Biolasol is a newly developed preserving solution for cold organ storage prior to transplantation. To date, only animal model experiments results are available. The aim of this single‐center analysis was to summarize the clinical experience concerning the early post‐transplant course of kidney grafts preserved with Biolasol in comparison with other preservation solutions. Before transplantation, 173 kidney grafts were preserved using Biolasol and 240 organs with other solutions (University of Wisconsin—UW, Institute Georges Lopez—IGL‐1, or StoreProtect Plus solutions). Early graft function was defined based on serum creatinine concentration at day 3 (<3 mg/dL—immediate graft function, IGF or >3 mg/dL—slow graft function, SGF) or the need of dialysis therapy during first post‐operative week (delayed graft function, DGF). The analysis included intrarenal resistive indices measured by Doppler sonography early after transplantation and before discharge from the hospital. IGF was more frequent in patients with organs preserved with IGL‐1 (33.5%) and StoreProtect Plus (38.8%) than Biolasol (18.5%), whereas there was no difference in the occurrence of DGF. Both initial and discharge median resistance index values were significantly higher in the Biolasol subgroup (0.77 and 0.75) than in all three other subgroups (P values for all comparisons <.001), also after 1:1 propensity score matching for baseline characteristics. Multiple logistic regression analysis based on the propensity score‐matched cohort revealed that the use of Biolasol solution [OR 0.59 (0.35‐0.98); P < .05] independently decreased the occurrence of IGF. In our single‐center clinical experience, kidney preservation using Biolasol solution was associated with significantly higher intrarenal resistant index in comparison with other preservation fluids, as well as worse early graft function than in the IGL‐1 and the StoreProtect Plus subgroups. Long‐term follow‐up is needed in order to assess the kidney graft and patient survival.

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“…It contains electrolytes, osmotically and oncotically active substances, buffering systems, substances that prevent cellular acidosis, which constitute energy sources, and antioxidants. The fluid was subjected to a pre-clinical study to assess its effectiveness and safety profile with the use of Polish large white pigs (Budziński et al, 2014a;Budziński et al, 2014b;Caban et al, 2014). The impact of the fluid on organ swelling and selected biochemical indicators during perfusion, after 24 hours of storage and reperfusion, was studied on 40 kidneys, 10 livers and 10 pancreases, divided into 2 groups.…”

Section: Fluids For Rinsing and Storing Organsmentioning

confidence: 99%

Tips for optimizing organ preservation solutions

2018

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Organ injury during ischemia is one of the clinical problems of today's transplantation. It occurs during warm ischemia time (WIT) when the blood flow is cut off and during cold ischemia when a graft is chilled in situ until the circulation is restored to the recipient organism. Fast cooling of the organ slows down metabolism and activates intracellular enzymes, which minimizes the effects of warm ischemia. Unfortunately, hypothermia also results in inhibition of ATP synthesis, cell swelling and intracellular acidity. That is why research is continually being conducted to develop new fluids for rinsing and storing organs, as well as to optimize the composition of those that are already in use, which will allow for longer and more effective graft storage and restoration of their optimal functions after transplantation. This article provides current information on rinsing and storage fluids available on the global market. It also discusses tips for the fluid modifications with hormones and micronutrients.

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Interleukin-6 Concentration in the Transgenic Pig's Liver Preserved for 24 Hours in Biolasol Solution

Cited by 6 publications

References 10 publications

Biochemical Studies in Perfundates and Homogenates of Isolated Porcine Kidneys after Flushing with Zinc or Zinc–Prolactin Modified Preservation Solution Using a Static Cold Storage Technique

Biochemical Studies in Perfundates and Homogenates of Isolated Porcine Kidneys after Flushing with Zinc or Zinc–Prolactin Modified Preservation Solution Using a Static Cold Storage Technique

Early graft function and intrarenal resistant index after kidney transplantation using Biolasol—A new solid organ preservation fluid

Tips for optimizing organ preservation solutions

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